The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Cuscutae Semen Extract on Prevention of Osteoporosis in Ovariectomized Rats

토사자(兎絲子)가 난소적출(卵巢摘出)로 유도(誘導)된 흰쥐의 골다공증(骨多孔症)에 미치는 영향(影響)

  • Kim, Jong-Hyun (Dept. of Anatomy, College of Korean Medicine, Institute of Korean Medicine, Kyung-Hee University) ;
  • Kim, Ho-Seok (Dept. of Anatomy, College of Korean Medicine, Institute of Korean Medicine, Kyung-Hee University) ;
  • Jun, Jae-Yun (Dept. of Anatomy, College of Korean Medicine, Institute of Korean Medicine, Kyung-Hee University) ;
  • Jeong, Da-Won (Dept. of Anatomy, College of Korean Medicine, Institute of Korean Medicine, Kyung-Hee University) ;
  • Cho, Chang-Young (Dept. of Anatomy, College of Korean Medicine, Institute of Korean Medicine, Kyung-Hee University) ;
  • Sohn, Young-Joo (Dept. of Anatomy, College of Korean Medicine, Institute of Korean Medicine, Kyung-Hee University) ;
  • Jung, Hyuk-Sang (Dept. of Anatomy, College of Korean Medicine, Institute of Korean Medicine, Kyung-Hee University)
  • 김종현 (경희대학교 한의과대학 해부학 교실) ;
  • 김호석 (경희대학교 한의과대학 해부학 교실) ;
  • 전재윤 (경희대학교 한의과대학 해부학 교실) ;
  • 정다원 (경희대학교 한의과대학 해부학 교실) ;
  • 조창영 (경희대학교 한의과대학 해부학 교실) ;
  • 손영주 (경희대학교 한의과대학 해부학 교실) ;
  • 정혁상 (경희대학교 한의과대학 해부학 교실)
  • Received : 2012.09.11
  • Accepted : 2012.11.10
  • Published : 2012.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: Osteoporosis, which occurs after menopause, is a kind of metabolic bone disorder. It develops when the bone mass begins to decrease radically, and its main symptoms are bone fracture and height-shortening. This thesis aims at what effects the Cuscutae Semen Extract(CSE) has on the prevention of osteoporosis in SD-rat that is caused by ovariectomy. Methods: The 24 female white rats, after removing their ovaries, were divided into the Normals, the Control group, and the CSE administrated group. For the next 8 weeks, distilled water to the normals and the control, and the CSE(45.9 mg/100 g) to the CSE administrated group were given in the mouths of them. After 8 weeks the rats were sacrificed. Weight, albumin, aspartate aminotransferase, alanine aminotransferase, total-cholesterol, triglyceride, phosphorus, calcium, tetraiodothyronine, estradiol, the weight of the femur, the amount of tibia ash, the area of trabecular bone, and the thickness of trabecular bone were measured. Results: The serum analysis shows that the calcium and phosphorous of the CSE administrated group significantly increased compared to that of the control group. Aspartate aminotransferase, alkalinephosphatase, total cholesterol, tetraiodothyronine of CSE group were decreased, but not so significant. Albumin, alanine aminotransferase, triglyceride and estradiol of the group showed the tendency to increase, but the significance wasn't admitted. Regarding the variation of bone, the femur weight and the ash content of tibia, CSE group was more increased than the control group but the significance wasn't admitted. The histological observation shows that the trabecular thickness was more increased than the control group, and trabecular area increased significantly compared to those of the control group. The number of osteoclast and osteoblast area of the CSE groups decreased significantly compared to those of the control group. Conclusions: From the result of the above study, CSE should be effective for the osteoporosis cure and precaution and deeper study through bedside and clinical demonstration is much needed from now on.

Keywords

Acknowledgement

Supported by : 경희대학교

References

  1. 대한병리학회. 병리학. 서울:고문사. 1990 :1177-85.
  2. 대한정형외과학회. 정형외과학. 서울: 최신의학사. 1992:13-4, 81-2.
  3. 한방재활의학과학회. 한방재활의학과학. 서울:군자출판사. 2003;4:105-6.
  4. 조수현. 폐경과 골다공증. 대한의학협회지. 1992;35(5):587-98.
  5. 두호경. 임상신계학연구. 서울:성보사. 1995:583-600.
  6. 何招奇. 現大中醫內科學. 北京:中國韓藥科枝出板社. 1992:578-80.
  7. 蔣位庄, 王和嗚. 中醫滑病學. 北京:人 民衛生出板社. 1994:255-8.
  8. 전국한의과대학 공동교재편집위원회. 본초학. 서울:영림사. 2005:620-1.
  9. Dong Hyun, K. Pharmacology of traditional Oriental medicine. Shin il books. 2005:690-1.
  10. Jinfeng Z. Experimental and clinical studies on the effect of Shou Tai Wan and additives on threatened abortion. Journal of Integrated Traditional and Western Medicine. 1987;7:407.
  11. Linmao Y. Integrating Chinese and western medicine to treat Infantile uterus. Herbal Journal of Traditional Chinese Medicine. 1992;14(4):40.
  12. Xiumei D. Researches on Semen Cuscutae botanicals. Research and Development of Chinese medicine in Chang Bai Shan. 1992;1(2):53.
  13. 해리슨내과학편찬위원회. HARRISON'S 내과학(2권). 서울:정담출판사. 1997: 2310-48.
  14. 두호경. 동의신계내과학. 서울:동양의학연구원. 1989:654-60.
  15. 대한골대사학회. 골다공증(골조송증). 서울:최신의학사. 1991:3-79.
  16. 아주대학교갱년기연구회. 최신갱년기 관리. 서울:연구사. 1996;31:109-46.
  17. 교과서편찬위원회. 부인과학(제 3판). 서울:칼빈서적. 1991:330-1.
  18. Aitken JM, et al. Osteoporosis after oophorectomy in the mature female rat and the effect of oestrogen and-or progestogen replacement therapy in its prevention. J Endocrinol. 1972;55(1) :79-87. https://doi.org/10.1677/joe.0.0550079
  19. 이귀녕, 이종순. 임상병리파일. 서울: 의학문화사. 1993.
  20. 간계내과학교수. 간계내과학. 서울:동양의학연구원. 1991:210-9.
  21. Morris JC, et al. Effect of oophorectomy on biochemical and bone variables in the rat. Bone and mineral. 1992;18 :133-42. https://doi.org/10.1016/0169-6009(92)90853-6
  22. 김순호 등. 최신임상검사진단학. 서울: 계측문화사. 1996:294-7.
  23. Vaughn. Estrogen replcement therapy. clin obstet gynecol. 1981;24(1):253-83. https://doi.org/10.1097/00003081-198103000-00023
  24. 강세윤. 오늘의 진단 및 치료. 서울: 도서출판 한우리. 1999:1295-6.
  25. 하승우. 골다공증의 치료 골흡수 억제제. 경북대학교 내분비 대사내과골다공증 논문집. 1995:51-5.
  26. James K, et al. Effects of 17 b-estradiol replacement and treadmill excerise on vertebral and femoral bones of the ovariectomized rat. Bone and mineral. 1994;24:223, 234.
  27. 이정재 등. 당귀지황음이 난소적출 백서의 골다공증에 미치는 영향. 대한한방부인과학회지. 2004;17(1):111-28.
  28. Ladizesky MG, et al. Effect of melatonin on bone metabolism in ovariectomized rats. Life Sci. 2001;70:557-65. https://doi.org/10.1016/S0024-3205(01)01431-X
  29. Shim JG, et al. Bone loss preventing effect of sophorae Fructus on ovariectomized rats. Arc Pharm Res. 2005;28:106-10. https://doi.org/10.1007/BF02975144
  30. Anthony M, et al. Relationship of fasting urinary calcium to circulating estrogen and body weight in postmenopausal women. JCM&M. 1980;50(1):70-5.
  31. Preteux H, et al. Mathemetical morphological study of lumbar spongious bone in age-related osteopenia. Calcif Tissue Int. 1983;35(1):48. https://doi.org/10.1007/BF02405006
  32. 蔡新吉. 腎虛證患者骨鑛物質含量改變 的初步的探討. 1994;14(3):154.
  33. 정국영 등. 삼수가 난소적출로 유발된 흰쥐의 골다공증에 미치는 영향. 대한본초학회지. 2003;18(4):12-23.
  34. Durbridge, et al. Progressive cancellous bone loss in rats after adrenaectomy and oophorectomy. Calcif. Tissue int. 1990:47-383. https://doi.org/10.1007/BF02555891