Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a Xylanolytic Bacterium, Bacillus sp. MX47

Xylanase 생산균 Bacillus sp. MX47의 분리 및 동정

  • Chi, Won-Jae (Department of Biological Science, Myongji University) ;
  • Park, Da Yeon (Department of Biological Science, Myongji University) ;
  • Park, Jae-Seon (Department of Biological Science, Myongji University) ;
  • Hong, Soon-Kwang (Department of Biological Science, Myongji University)
  • 지원재 (명지대학교 생명과학정보학부) ;
  • 박다연 (명지대학교 생명과학정보학부) ;
  • 박재선 (명지대학교 생명과학정보학부) ;
  • 홍순광 (명지대학교 생명과학정보학부)
  • Received : 2012.07.31
  • Accepted : 2012.10.04
  • Published : 2012.12.28
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Abstract

A xylanolytic bacterial strain, MX47, was isolated from rotting plant matter in soil. The strain was aerobic and gram positive, and grew between pH 6.0 and 11.0. Cells were susceptible to thiostrepton and chloramphenicol. The major fatty acids (>3%) comprised 64.55% of iso-$C_{15:0}$, 22.76% of anteiso-$C_{15:0}$, and 3.92% of iso-$C_{17:0}$. The G/C content of the DNA was 44.15 mol%. The predominant respiratory quinone was menaquinone 7 (MK-7). Searches for 16S rRNA gene sequence similarity as well as phylogenetic analyses strongly suggested that the strain should be classified to the genus Bacillus. However, its biochemical characteristics, including acid production and enzyme activities, are different from those of other Bacillus strains in the same clade, and therefore, we propose the name Bacillus sp. MX47.

Keywords

References

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