Experimental and Molecular Medicine

Korean Society for Biochemistry and Molecular Biongy (생화학분자생물학회)
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Identification of novel peptides that stimulate human neutrophils

  • Bae, Geon Ho (Department of Biological Sciences, Sungkyunkwan University) ;
  • Lee, Ha Young (Department of Biological Sciences, Sungkyunkwan University) ;
  • Jung, Young Su (Department of Biological Sciences, Sungkyunkwan University) ;
  • Shim, Jae Woong (Department of Biological Sciences, Sungkyunkwan University) ;
  • Kim, Sang Doo (Department of Biological Sciences, Sungkyunkwan University) ;
  • Baek, Suk-Hwan (Department of Biochemistry and Molecular Biology, Yeungnam University) ;
  • Kwon, Jae Young (Department of Biological Sciences, Sungkyunkwan University) ;
  • Park, Joon Seong (Department of Hematology-Oncology, Ajou University School of Medicine) ;
  • Bae, Yoe-Sik (Department of Biological Sciences, Sungkyunkwan University)
  • Accepted : 2011.11.14
  • Published : 2012.02.29
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Abstract

Neutrophils play a key role in innate immunity, and the identification of new stimuli that stimulate neutrophil activity is a very important issue. In this study, we identified three novel peptides by screening a synthetic hexapeptide combinatorial library. The identified peptides GMMWAI, MMHWAM, and MMHWFM caused an increase in intracellular $Ca^{2+}$ in a concentration-dependent manner via phospholipase C activity in human neutrophils. The three peptides acted specifically on neutrophils and monocytes and not on other non-leukocytic cells. As a physiological characteristic of the peptides, we observed that the three peptides induced chemotactic migration of neutrophils as well as stimulated superoxide anion production. Studying receptor specificity, we observed that two of the peptides (GMMWAI and MMHWFM) acted on formyl peptide receptor (FPR)1 while the other peptide (MMHWAM) acted on FPR2. Since the three novel peptides were specific agonists for FPR1 or FPR2, they might be useful tools to study FPR1- or FPR2-mediated immune response and signaling.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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