Experimental and Molecular Medicine

Korean Society for Biochemistry and Molecular Biongy (생화학분자생물학회)
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Galectin-3 increases the motility of mouse melanoma cells by regulating matrix metalloproteinase-1 expression

  • Wang, Yuan-Guo (Department of Practical Pharmacy, College of Pharmacy, Kyung Hee University) ;
  • Kim, Seok-Jun (Cancer Genomics Branch, Division of Convergence Technology, National Cancer Center Research Institute and Hospital) ;
  • Baek, Jung-Hwan (Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine) ;
  • Lee, Hyun-Woo (Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine) ;
  • Jeong, Seo-Young (Department of Practical Pharmacy, College of Pharmacy, Kyung Hee University) ;
  • Chun, Kyung-Hee (Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine)
  • Accepted : 2012.03.12
  • Published : 2012.06.30
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Abstract

Although mounting evidence indicates the involvement of galectin-3 in cancer progression and metastasis, the underlying molecular mechanisms remain largely unknown. In this study, we investigated the effect and possible mechanism of galectin-3 on the migration and invasion of B16F10, a metastatic melanoma cell line, in which galectin-3 and matrix metalloproteinase-1 (MMP-1) were both found to be highly expressed. Knockdown of galectin-3 with specific siRNA reduced migration and invasion, which was associated with reduced expression of MMP-1. To further investigate the underlying mechanism, we examined the effect of galectin-3 knockdown on the activity of AP-1, a transcriptional factor regulating MMP-1 expression. We found that galectin-3 directly interacted with AP-1 and facilitated the binding of this complex to the MMP-1 promoter that drives MMP-1 transcription. Moreover, silencing of galectin-3 inhibited binding of fra-1 and c-Jun to promoter sites of MMP-1 gene. Consistent with these in vitro findings, our in vivo study demonstrated that galectin-3 shRNA treatment significantly reduced the total number of mouse lung metastatic nodules. Taken together, galectin-3 facilitates cell migration and invasion in melanoma in vitro and can induce metastasis in vivo, in part through, regulating the transcription activity of AP-1 and thereby up-regulating MMP-1 expression.

Keywords

Acknowledgement

Supported by : Ministry of Health & Welfare

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