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The Antioxidant Activity of Yacon (Polymnia sonchifoliaty) and its Application to the Pork Patties as a Natural Antioxidant

야콘 착즙액의 항산화 활성 및 천연 항산화제로서 돈육패티에 이용

  • Park, Jin-Sun (Department of Animal Science and Biotechnology Research Institute, Chonnam National University) ;
  • Kim, Hyeong-Sang (Department of Animal Science and Biotechnology Research Institute, Chonnam National University) ;
  • Chin, Koo-Bok (Department of Animal Science and Biotechnology Research Institute, Chonnam National University)
  • 박진선 (전남대학교 동물자원학부 및 생물공학 연구소) ;
  • 김형상 (전남대학교 동물자원학부 및 생물공학 연구소) ;
  • 진구복 (전남대학교 동물자원학부 및 생물공학 연구소)
  • Received : 2011.11.29
  • Accepted : 2012.02.21
  • Published : 2012.04.30

Abstract

This study was performed to evaluate the extraction method (Yacon ethanol extracts; YEE, Yacon pressed extracts; YPE) and various levels (0.05-1.0%) of Yacon (Polymnia sonchifolia) on antioxidant and antimicrobial activities. In linoleic acid emulsion of YPE, there were higher iron chelation activity and antioxidant activity than those of YEE (p<0.05). A 1,1- diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity and reducing power of both extracts showed a higher rate at 0.5% level. Ground pork patties, which contain 0.5% YEE and YPE, were manufactured and BHT (0.01%) was used as a reference. Physicochemical properties and microbial counts of ground pork patties, containing a different type of Yacon, were evaluated during the 14 d of storage at $4^{\circ}C$. A pH level, and lightness (Hunter L), as well as the yellowness (Hunter b) values of treatments were not different from those of the control (p>0.05), but increased during storage, at $4^{\circ}C$. Lightness values of ground pork patties, with Yacon extracts, showed the highest. TBARS value of ground pork patties that contains Yacon increased with increased storage at $4^{\circ}C$ (p<0.05), and pork patties with YPE or YEE retarded the lipid oxidation, during refrigerated storage, as compared to that of the CTL. Thus, YPE could be used as a potential possibility to inhibit the lipid oxidation of processed meats, during the refrigerated storage.

본 연구는 국화과 구근 작물인 야콘을 이용하여 농도별(0.05, 0.1, 0.25, 0.5, 1%)로 야콘 에탄올 추출물(YEE)과 야콘 착즙액(YPE)의 항산화 활성을 평가했다. 그 결과 철이온 흡착력과 linoleic acid 과산화 억제능 및 추출 수율에서 야콘 착즙액이 야콘 에탄올 추출물보다 더 높은 활성을 나타내었다(p<0.05). 특히 철이온 흡착력에서 YPE가 0.5% 수준에서 약 80% 정도의 활성을 보였고, linoleic acid 과산화 억제능에서는 70% 이상의 매우 높은 활성을 띄었다. DPPH 라디칼 소거능과 환원력에서는 두 추출물 모두 0.5% 수준 이상에서 높은 활성을 보였다(p>0.05). 이러한 결과를 토대로 0.5%의 YEE와 YPE를 각각 돈육패티에 적용하였고, 대조구로는 BHT 0.01%를 이용하여 냉장저장 기간 동안(0, 3, 7, 10, 14일) 이화학적 특성 및 미생물 검사를 실시하였다. 그 결과 pH와 백색도, 황색도에서 각 처리구에 따른 유의적인 차이를 보이지 않았으나(p>0.05) 저장기간이 경과함에 따라 증가하는 경향을 보였고, 반면 적색도에서는 처리구별로 대조구가 가장 높고 REF(BHT 0.01%), TRT1(YEE), TRT2(YPE) 순서로 낮아지는 경향을 보였으나 10일이후부터는 대조구와 유의차가 없었고(p>0.05), 저장기간이 경과할수록 유의적으로 낮아졌다(p<0.05). 미생물 결과는 처리구별 차이는 없었으나(p>0.05), 7일 경과 후부터 모든 처리구가 급격히 증가하였다(p<0.05). TBARS값도 저장기간이 경과함에 따라 증가하는 경향을 보였으며(p<0.05), 야콘 착즙액 처리구와 에탄올 추출물 처리구간에 차이는 없었으나(p>0.05), 착즙액의 경우 대조구에 비하여 낮음으로써 항산화 활성을 보였다(p>0.05). 이와 같은 연구결과로 볼 때 야콘 착즙액이 지방 산화억제를 위한 천연의 항산화제로 식육가공품에 사용 가능성이 있을 것으로 판단된다.

Keywords

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