Journal of Haehwa Medicine (혜화의학회지)

Research Institute of Korean Medicine (대전대학교 한의학연구소)
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Effect of SHT on the anti-oxidant activity and atopic dermatitis related inflammatory cytokines

석자해기탕(石紫解肌湯)이 아토피피부염 유관 인자인 산화적 손상과 염증 인자에 미치는 영향

  • Jung, Dae-Woong (Dept. of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Choi, Hak-Joo (Traditional and Biomedical Research Center(TBRC), Daejeon University) ;
  • Gim, Seon-Bin (Traditional and Biomedical Research Center(TBRC), Daejeon University) ;
  • Lee, Ki-Moo (Doori Cosmetics) ;
  • Kim, Dong-Hee (Dept. of Pathology, College of Oriental Medicine, Daejeon University)
  • Received : 2011.12.23
  • Accepted : 2012.02.02
  • Published : 2012.02.20
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Abstract

In order to investigate the possibility of SHT as therapeutic for the treatment of atopic dermatitis (AD), cytotoxicity, anti-oxidant activity, modulatory and suppression activities of SHT were tested. 90% or higher cell viability was observed in all tested groups from 25 to 200 ug/ml using Raw 264.7 cells. SHT showed dose-dependent DPPH scavenging activity, with more than 80% scavenging activities at 400 and 800 ug/ml concentrations. SHT showed dose-dependent suppression activity of ROS production, especially at 200 ug/ml of 57.4%. SHT decreased NO production activity dose dependently, expecially at 200 ug/ml of 28.8%. IL-$1{\beta}$, IL-6, MCP-1, TNF-${\alpha}$ production rate were decreased by 45.7%, 15.5%, 8.9%, 16.5% respectively when Raw 264.7 cells were treated with LPS and with SHT of 200 ug/ml. However, only IL-6 and TNF-${\alpha}$ showed significant changes. The results above indicate that SHT significantly reduces the effect of oxidative and inflammatory cytokines. The use of SHT in dermatitis can be widely suggested.

Keywords

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