Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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Effect of Chrysin on Gene Expression and Production of MUC5AC Mucin from Cultured Airway Epithelial Cells

  • Shin, Hyun-Dae (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Lee, Hyun Jae (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Sikder, Asaduzzaman Md. (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Park, Su Hyun (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Ryu, Jiho (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Hong, Jang-Hee (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Kim, Ju-Ock (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Seok, Jeong Ho (Department of Pharmacology, Chungnam National University School of Medicine) ;
  • Lee, Choong Jae (Department of Pharmacology, Chungnam National University School of Medicine)
  • Received : 2012.07.31
  • Accepted : 2012.09.13
  • Published : 2012.10.30
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Abstract

Background: We investigated whether chrysin affected MUC5AC mucin production and gene expression induced by phorbol ester (phorbol 12-myristate 13-acetate, PMA) or epidermal growth factor (EGF) from human airway epithelial cells. Methods: Confluent NCI-H292 cells were pretreated with varying concentrations of chrysin for 30 minutes, and were then stimulated with PMA and EGF for 24 hours, respectively. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Results: Concentrations of $10{\mu}M$ and $100{\mu}M$ chrysin were found to inhibit the production of MUC5AC mucin protein induced by PMA; A concentration of $100{\mu}M$ chrysin also inhibited the production of MUC5AC mucin protein induced by EGF; $100{\mu}M$ chrysin inhibited the expression of MUC5AC mucin gene induced by PMA or EGF. The cytotoxicity of chrysin was checked by lactate dehydrogenase assay, and there was no cytotoxic effect observed for chrysin. Conclusion: These results suggest that chrysin can inhibit mucin gene expression and the production of mucin protein by directly acting on airway epithelial cells.

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Acknowledgement

Supported by : Chungnam National University Hospital

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