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Physiochemical and Microbiological Changes of the Fermented Dandelion (Taraxacum officinale) Extracts with Raw Sugar

당 첨가 민들레(Taraxacum officinale) 발효 추출물의 발효과정 중 이화학적 및 미생물학적 변화

  • Kim, Kyung-Min (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Kim, Young-Nam (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research & Experiment Services) ;
  • Choi, Byoung-Kon (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research & Experiment Services) ;
  • Oh, Deog-Hwan (Dept. of Food Science and Biotechnology, School of Biotechnology and Bioengineering and Institute of Bioscience & Biotechnology, Kangwon National University)
  • 김경민 (강원대학교 바이오산업공학부 식품생명공학과) ;
  • 김영남 (강원도농업기술원 농산물이용시험) ;
  • 최병곤 (강원도농업기술원 농산물이용시험) ;
  • 오덕환 (강원대학교 바이오산업공학부 식품생명공학과)
  • Received : 2011.10.05
  • Accepted : 2012.01.27
  • Published : 2012.02.28

Abstract

This study was carried out to investigate the physiochemical and microbiological changes of dandelion during fermentation. Thirty and fifty percentage raw sugar groups (SFE30 and SFE50) were introduced into dandelions and fermented for 120 days at $15-20^{\circ}C$. This study was conducted to investigate the utilization of sucrose on lactic acid bacteria from dandelions and their effect on the pH, titratable acidity, microorganism and formation of organic acids in dandelions during fermentation. The number of lactic acid bacteria increased remarkably up to 15 days of fermentation and then decreased rapidly thereafter. The maximum number of lactic acid bacteria, 7.9 log CFU/mL was reached at pH 4.17 and the pH of dandelion showed a slight decrease during fermentation and decreased steadily up to 90 days to reach an optimum pH of 4.0. The titratable acidity of dandelions fermented increased during fermentation. The concentration of organic acid, amino acid and free sugar in SFE30 was higher than both SFE50 and DWE. The results of dandelions fermented were remarkably retarded in the 50% raw sugar group compared to the 30% raw sugar group.

본 연구는 민들레에 당을 첨가 후 젖산발효를 시켜 발효과정 중의 이화학적, 미생물학적 변화를 탐구하였고 최종 발효물의 성분분석을 통해 기능성 음료로서의 가치를 평가하기 위해 연구를 진행했다. 30% 당을 첨가하여 발효시킨 민들레 발효물 (FD30)과 50% 당을 첨가하여 발효시킨 민들레 발효물 (FD50)은 발효가 진행됨에 따라 FD30의 초기 pH 및 산도가 각각 pH 6.4, 0.18%에서 숙성 30일에 pH 3.85 및 0.72%로 변화되었고, FD50 보다 저하 속도가 다소 빠른 경향을 보였으며 숙성 120일 이후에는 안정된 값을 나타내었다. 발효과정에 따른 총 균수 변화는 Soluble solid와 상관없이 초기에는 비슷한 총 균수를 나타냈고, 점차로 증가하여 최대균수에 도달한 후 다시 서서히 감소하는 발효양상을 나타냈다. 유산균수 결과도 총 균수의 결과와 비슷한 양상을 보였고 유산균수가 초기에 크게 증가하였다가 최대 유산균수를 보인 후 서서히 감소했다. 발효 및 숙성이 완료된 민들레 발효물의 유리당 함량은 각 농도별의 처리구 간에서 fructose, sucrose의 함량은 FD30, FD50 간에 큰 변화를 보이지 않았으나 glucose의 경우에는 FD50이 $46.86{\pm}1.93%$로 FD30에 비하여 2배 이상 증가하였다. 유기산 함량 분석결과 FD30이 FD50보다 유기산의 종류가 다양하고 함량이 높은 것으로 나타났고 유리아미노산 함량은 유기산의 경우와 같은 양상을 보였으며 발효물 모두 민들레물 추출물 (DWE)보다 많은 유기산과 유리아미노산을 함유했다. 이는 민들레에 함유된 각종 효소들과 미생물이 당 발효에 의해 유용한 작용을 거쳐 주요성분이 분해되고, 또한 재합성이 이루어져 각종 유리당, 유기산 및 유리아미노산 등을 생성한다고 판단된다. 따라서 발효를 통한 민들레의 기능성 식품으로의 활용가치를 기대할 수 있고 발효시 첨가물과 시기에 따른 성분변화에 대한 보다 구체적인 연구가 추가로 진행되어야 할 것으로 사료된다.

Keywords

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