The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

A Study on the Acute Toxicity of Leejung-tang(Lizhong-tang) and Fermented Leejung-tang(Lizhong-tang) Extract in ICR Mice

ICR 마우스를 이용한 이중탕 및 발효이중탕의 급성독성 연구

  • Jung, Young-Pil (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Hwang, Yun-Hwan (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Lee, Ji-Hye (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Yim, Nam-Hui (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Cho, Won-Kyung (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Ma, Jin-Yeul (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
  • 정영필 (한국한의학연구원 한의신약연구그룹) ;
  • 황윤환 (한국한의학연구원 한의신약연구그룹) ;
  • 이지혜 (한국한의학연구원 한의신약연구그룹) ;
  • 임남희 (한국한의학연구원 한의신약연구그룹) ;
  • 조원경 (한국한의학연구원 한의신약연구그룹) ;
  • 마진열 (한국한의학연구원 한의신약연구그룹)
  • Received : 2012.04.18
  • Accepted : 2012.04.27
  • Published : 2012.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was carried out to investigate the acute toxicity and safety of $Leejung$-$tang$($Lizhong$-$tang$) and fermented $Leejung$-$tang$($Lizhong$-$tang$) extract. Methods : To evaluate their acute toxicity and safety, 0(control group), 1250, 2500 and 5000 mg/kg of $Leejung$-$tang$($Lizhong$-$tang$) and fermented $Leejung$-$tang$($Lizhong$-$tang$) extracts were orally administered to 35 male and 35 female ICR mice. After a single administration, we observed survival rates, general toxicity, changes of body weight, and autopsy. Results : Compared with the control group, we could not find any toxic alteration in any of treated groups (1250, 2500 and 5000 mg/kg). Conclusions : $LD_{50}$ of $Leejung$-$tang$($Lizhong$-$tang$) and fermented $Leejung$-$tang$($Lizhong$-$tang$) were over 5000 mg/kg and it is very safe to ICR mice.

Keywords

References

  1. Ko MG, Jang MK. Hyundae-Sanghanron. Seoul： Haneuimunhwasa. 2000：647-9.
  2. Kang MK. Jejungsinpyen. Seoul：Haenglimseowon. 1975：150.
  3. Huh J. Donguibogam. Seoul：Bubinmunhwasa. 2002：1009
  4. Hwang DY. Daeyuekjeugmek-Bangyackhappyen. Seoul：Namsandang. 1992： 127-8.
  5. Kim JS, Yoo BH, Park DW, Yoo GW. An experimental studies on the effects of Leejungtang. K.H.M. 1996；2(2)：167-79.
  6. Kwak KH, Yoo BH, Yoo KW. Experimental studies on the effects of Yijoongtang and Daeseunggitang. K.H.Univ.O.Med.J. 1987；10(1)：505-29.
  7. Park YH. Lizhong-tang on over weight syndrom:A case report. J Kor Med Obes Res 2001；1(1)： 43-5.
  8. Nam HJ, Chae BY. The experimental study in the anti-allergic effects of Leejungtang and Leejungtanggamibang. J Kor Ori Med ophthalmol otolaryngol dematol 2000；13(1)：60-77.
  9. Seo HY, Han JK, Kim YH. The effect of Li zhong tang on the suppression of Th2 dfferentiation by IFN-γ response in IgE hyperproduction and atopic dermatitis-like skin lesions induced NC/Nga mouse. J kor ori pediatr 2009；23(1)：1-22.
  10. Seo HY, Han JK, Kim YH. Therapeutic effects of Yijungtang on atopic dermatitis-like skin lesions of NC/Nga mouse induced by mite antigen. J kor ori pediatr. 2011；25(1)：1-27.
  11. Ha JY, Lee J. Experiment on the Antitumor and Immunomodulatory Effects of Gagamijungtang in BALB/c mice. Kor J ori Physiol pathol. 1998； 12(2)：73-81.
  12. Park WS. Effects of red ginseng-ejung-tang and white ginseng-ejung-tang water extract on hydrogen peroxide production in RAW 264.7 cells. Kor J ori Physiol pathol. 2011；25(1)：78-83.
  13. Lee JY, Kim YJ, Park WS. Effects of White Ginseng-Ejung-tang Acupuncture Solution on Nitric Oxide and Hydrogen Peroxide Production in LPS-induced Mouse Macrophages. Kor J Acupunc. 2011；28(1)：61-9.
  14. Kim TG, Park SM, Kang H, Shim BS, Kim SH, Choi SH, et al. Effect of baekhasuoyijung-tang on mouse T cell cytokines. Kor J Ori physiol pathol 2008；22(4)：754-61.
  15. Lee SM, Choi IH. Therapeutic effects of Lizhongtang plus baidusan extract in rats with allergic rhinitis. J Kor Ori Med ophthalmol otolaryngol dematol 2004；17(2)：72-80.
  16. Kim KS, Ryu SN. Current status and prospects for the quality evaluation of medicinal plants. Korean J crop sci. 2002；47(S)：186-99.
  17. Bae EA, Han MJ, Kim EJ, Kim DH. Transformation of ginseng saponins to ginsenoside Rh2 by acids and human intestinal bacteria and biological activities of their transformants. Arch Pharm Res. 2004； 27(1)：61-7. https://doi.org/10.1007/BF02980048
  18. Park JH, Kim HJ, Lee MJ. The role of gut microbiota in obesity and utilization of fermented herbal extracts. J Kor Med Obes Res. 2009；9(1)： 1-14.
  19. Jang IS, Yang CS, Lee SD, Han CH. A review of herbal medicinal products associated with toxic events in korea. J kor ori med. 2007；28(1)：1-10
  20. Hayes AW. Principles and methods of toxicology. New York：Raven press. 1984：17-9.
  21. MA JY, Huang DS, Yu YB, Ha HK, Shin HK. Acute toxicity study on Sagunja-tang(Sijunzi-tang) in ICR mice. J kor ori med. 2007；28(2)：200-4
  22. U.S. Enviromental Protection Agency. Health Effects Test Guidelines OPPTS 870. 100, Acute Toxicity Testing Background. Washington：US EPA. 1998.
  23. Lee SI, Kim DC, Lee YJ, No SH, Joo YS. Gwangjujungeuihakwon-Jupyen. Seoul：Younglimsa. 1990：144-6.
  24. Cho SI, Kim HW, Lee GJ. Biological activities of extracts of fermented camellia japonica leaf and flower. Kor J herbology. 2006；21(2)：55-62.

Cited by

  1. Single Oral Dose Toxicity Test of Fermented Samchulgeonbi-tang Extract in ICR mice vol.28, pp.2, 2013, https://doi.org/10.6116/kjh.2013.28.2.61
  2. Simultaneous quantification and inhibitory effect on LDL oxidation of the traditional Korean medicine, Leejung-tang vol.14, pp.1, 2014, https://doi.org/10.1186/1472-6882-14-3
  3. LC-MS/MS를 이용한 이중탕(理中湯)의 정량분석 및 항산화 활성 vol.21, pp.1, 2013, https://doi.org/10.14374/hfs.2013.21.1.177