Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Stimulation of Dendritic Cell Maturation and Induction of Apoptosis in Leukemia Cells by a Heat-stable Extract from Azuki bean (Vigna angularis), a Promising Immunopotentiating Food and Dietary Supplement for Cancer Prevention

  • Nakaya, Kazuyasu (Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences) ;
  • Nabata, Yuri (Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences) ;
  • Ichiyanagi, Takashi (Cancer Research Institute, Harbin Medical University) ;
  • An, Wei Wei
  • Published : 2012.02.29
Download PDF
⟨ Previous Next ⟩

Abstract

Non-toxic stimulation of dendritic cells (DCs), which are central immunomodulators, may aid the prevention of cancer. Furthermore, induction of apoptosis in cancer cells by anticancer agents contributes to the induction of DC maturation. We previously reported that extracts from $Pinus$ $parviflora$ Sieb. et Zucc pine cone and $Mucuna$ seed induce differentiation of mouse bone marrow cells into mature dendritic cells and also induce apoptosis in various human cancer cell lines. In the present study, we screened 31 kinds of edible beans with biological activity similar to that of extracts from pine cone and $Mucuna$ and found that the heat-stable extract from azuki bean ($Vigna$ $angula$) stimulated differentiation of bone marrow cells into immature DCs with the greatest efficacy. The level of IL-6 produced by sequential treatment of DCs with azuki extract and lipopolysaccharide was the highest among the examined beans. Azuki extract also inhibited the growth of human leukemia U937 cells, leading to induction of apoptosis. These results suggest that azuki bean and its extract are immunopotentiating foods that can be used as a dietary supplement for cancer prevention and immunotherapy.

Keywords

References

  1. Adlercreutz H (2002). Phytoestrogens and breast cancer. J Steroid Biochem Mol Biol, 83, 113-8. https://doi.org/10.1016/S0960-0760(02)00273-X
  2. An W-W, Kanazawa Y, Ozawa M, et al (2010). Dendritic cell differentiation and tumor cell apoptosis induced by components of a polyphenylpropanoid polysaccharide complex. Anticancer Res, 30, 3-12.
  3. Apetoh L, Tesniere A, Ghiringhelli F, et al (2008). Molecular interactions between dying tumor cells and the innate immune system determine the efficacy of conventional anticancer therapies. Cancer Res, 68, 4026-30. https://doi.org/10.1158/0008-5472.CAN-08-0427
  4. Banchereau J, Steinman RM (1998). Dendritic cells and the control of immunity. Nature, 392, 245-52. https://doi.org/10.1038/32588
  5. Borchers AT, Keen CL, Gershwin ME (2004). Mushrooms, tumors, and immunity: an update. Exp Biol Med (Maywood), 229, 393-406. https://doi.org/10.1177/153537020422900507
  6. Hashimoto S, Takanashi N, Kajimoto T, et al (2002). Sophoranone, extract from a traditional Chinese medicine Shan Dou Gen, induces apoptosis in human leukemia U937 cells via formation of reactive oxygen species and opening of mitochondrial permeability transition pores. Int J Cancer, 99, 879-90. https://doi.org/10.1002/ijc.10414
  7. Itoh T, Itoh Y, Mizutani M, et al (2004). Hot-water extracts from adzuki beans (Vigna angularis) suppress not only the proliferation of KATO III cells in culture but also benzo($\alpha$) pyrene-induced tumorigenesis in mouse forestomatch. J Nutr Sci Vitamino. (Tokyo), 50, 295-9. https://doi.org/10.3177/jnsv.50.295
  8. Jeras M, Bergant M, Repnik U (2005). In vitro preparation and functional assessment of human monocyte-derived dendritic cells-potential antigen-specific modulators of in vivo immune responses. Transpl Immunol, 14, 231-44. https://doi.org/10.1016/j.trim.2005.03.012
  9. Kaufmann SH (1989). Induction of endonucleolytic DNA cleavage in human acute myelogenous leukemia cells by etoposide, camptothecin, and other cytotoxic anticancer drugs: a cautionary note. Cancer Res, 49, 5870-8.
  10. Kurokawa K, Ishii R, An W-W, et al (2011). A heat-stable extract from Mucuna stimulates the differentiation of bone marrow cells into dendritic cells and induces apoptosis in cancer cells. Nutr Cancer, 63, 100-8.
  11. Matsuba S, Matsuno H, Sakuma M, et al (2008). Phellinus linteus extract augments the immune response in mitomycin C-induced immunodeficient mice. Alternat Med, 5, 85-90.
  12. Obeid M, Tesniere A, Ghiringhelli F, et al (2007). Calreticulin exposure dictates the immunogenicity of cancer cell death. Nat Med, 13, 54-61. https://doi.org/10.1038/nm1523
  13. Park SK, Kim GY, Lim JY, et al (2003). Acidic polysaccharides isolated from Phellinus linteus induce phenotypic and functional maturation of murine dendritic cells. Biochem Biophys Res Commun, 312, 449-58. https://doi.org/10.1016/j.bbrc.2003.10.136
  14. Reinli K, Block G (1996). Phytoestrogens and breast cancer. Phytoestrogen content of foods--a compendium of literature values. Nutr Cancer, 26, 123-148. https://doi.org/10.1080/01635589609514470
  15. Tesniere A, Panaretakis T, Kepp O, et al (2008). Molecular characteristics of immunogenic cancer cell death. Cell Death Differ, 15, 3-12. https://doi.org/10.1038/sj.cdd.4402269
  16. Vicari AP, Caux C, Trinchieri G (2002). Tumour escape from immune surveillance through dendritic cell inactivation. Semin Cancer Biol, 12, 33-42. https://doi.org/10.1006/scbi.2001.0400
  17. Wuttke W, Jarry H, Seidlova-Wuttke D (2007). Isoflavones-- safe food additives or dangerous drugs? Ageing Res Rev, 6, 150-88. https://doi.org/10.1016/j.arr.2007.05.001
  18. Zitvogel L, Apetoh L, Ghiringhelli F, et al (2008). The anticancer immune response: indispensable for therapeutic success? J Clin Invest, 118, 1991-2001. https://doi.org/10.1172/JCI35180

Cited by

  1. Macrophage Migration Inhibitory Factor: a Potential Marker for Cancer Diagnosis and Therapy vol.13, pp.5, 2012, https://doi.org/10.7314/APJCP.2012.13.5.1737
  2. High glucose increases LPS-induced DC apoptosis through modulation of ERK1/2, AKT and Bax/Bcl-2 vol.14, pp.1, 2014, https://doi.org/10.1186/1471-230X-14-98
  3. Effect of aqueous extract of the Vigna angularis in rats subjected to an experimental model of moderate chronic kidney disease vol.31, pp.8, 2016, https://doi.org/10.1590/S0102-865020160080000005
  4. Alternol inhibits the proliferation and induces the differentiation of the mouse melanoma B16F0 cell line vol.36, pp.2, 2016, https://doi.org/10.3892/or.2016.4844
  5. Macrophage Migration Inhibitory Factor (MIF): Biological Activities and Relation with Cancer vol.23, pp.2, 2017, https://doi.org/10.1007/s12253-016-0138-6
  6. Common Beans and Their Non-Digestible Fraction: Cancer Inhibitory Activity—An Overview vol.2, pp.3, 2013, https://doi.org/10.3390/foods2030374