Korean Journal of Chemical Engineering

  • 제29권3호
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  • Pages.392-395
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  • 2012
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  • 0256-1115(pISSN)
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  • 1975-7220(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
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DOI QR코드

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Mathematical analysis of colonial formation of embryonic stem cells in microfluidic system

  • Min, Seul Ki (Department of Biological Engineering, Inha University) ;
  • Lee, Byung Man (Department of Biological Engineering, Inha University) ;
  • Hwang, Jin Ha (Department of Biological Engineering, Inha University) ;
  • Ha, Sung Ho (Departmet of Chemical Engineering & Nano-Bio Technology, Hannam University) ;
  • Shin, Hwa Sung (Department of Biological Engineering, Inha University)
  • 투고 : 2011.05.27
  • 심사 : 2011.07.15
  • 발행 : 2012.03.01
⟨ 이전 논문 다음 논문 ⟩

초록

A fluidic environment affects mechanochemical characteristics of embryonic stem cells (ESCs). Perfusion is recognized as an attractive culture mode of ESCs since the steady fluidic state can enhance ESCs' controllability, supporting a unique cell culture condition. Cellular membrane motility presents important information about cellular dynamics such as adhesion, spreading, and migration. Thus, an investigation of the perfusion-induced membrane motility is significant to understand the mechanochemical behavior of ESCs in the steady culture state. In this research, we suggest $L_{fr}$, the ratio of circumferential membrane unattached to other cells' to the cell's circumference, as a new parameter to characterize cells' shape and motility. $L_{fr}$ of embryonic stem cells has positive correlations with cellular area ($A_r$) and free peripheral length ($L_f$) but a negative correlation with roundness ($R_n$). We also propose a mathematical model representing ESCs' membrane motilities and demonstrate their colonical behavior.

키워드

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea (NRF)

참고문헌

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  3. Programmable Static Droplet Array for the Analysis of Cell–Cell Communication in a Confined Microenvironment vol.89, pp.18, 2012, https://doi.org/10.1021/acs.analchem.7b01462
  4. Asymmetrical breakup and size distribution of droplets in a branching microfluidic T-junction vol.36, pp.1, 2012, https://doi.org/10.1007/s11814-018-0165-y