한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제16권1호
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  • Pages.9-18
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  • 2012
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

Effects of 4-Nonlyphenol Exposure on $P.$ $olivaceus$ and $S.$ $schlegeli$ Vitellogenesis

  • Jung, Jee-Hyun (Oil and POPs Research Group, Korea Ocean Research and Development Institute) ;
  • Bae, Sun-Hae (Major of Molecular Biology, Division of Life Science, Dong-eui University) ;
  • Baeck, Kyong-Lan (Major of Molecular Biology, Division of Life Science, Dong-eui University) ;
  • Shim, Won-Joon (Oil and POPs Research Group, Korea Ocean Research and Development Institute) ;
  • Kim, Dae-Jung (New Strategy Research Center, National Fisheries Research and Development Institute) ;
  • Han, Chang-Hee (Major of Molecular Biology, Division of Life Science, Dong-eui University)
  • 투고 : 2011.12.11
  • 심사 : 2012.01.20
  • 발행 : 2012.03.31
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초록

The effects of the estrogenic compound 4-nonylphenol (4-NP) on vitellogenesis in juvenile olive flounder ($Paralichthys$ $olivaceus$) and rockfish ($Sebastes$ $schlegeli$) exposed continuously at 10, 50 and $100{\mu}g{\ell}^{-1}$ levels for 7 days were compared. The expression of VTG mRNA level and protein using specific probes were examined. The levels of plasma estradiol-17 ${\beta}(E_2)$ and testosterone (T) were assessed by radioimmunoassay (RIA). Plasma $E_2$ concentrations increased significantly in two female fish species exposed to $100{\mu}g{\ell}^{-1}$ of 4-NP over concentrations in control fish. Plasma T concentrations increased in $P.$ $olivaceus$. Four days after exposure, the level of VTG mRNA expression increased in $P.$ $olivaceus$ and $S.$ $schlegeli$ exposed to $20{\mu}g{\ell}^{-1}$ of 4-NP. In addition, plasma VTG protein expression was seen in $P.$ $olivaceus$ and $S.$ $schlegeli$. In $S.$ $schlegeli$ and $P.$ $olivaceus$ exposed to 4-NP, the changes were noticed mainly in hepatocytic vaculation after 7 days of exposure. Thus, 4-NP may disrupt vitellogenesis in immature fish both directly and indirectly via disrupted steroidogenesis and liver pathology. Immature $S.$ $schlegeli$ were the most sensitive to 4-NP exposure in vitellogenesis.

키워드

참고문헌

  1. Aida K, Kato T, Awaji M (1984) Effects of castration on the smoltification of precocious male masu salmon Oncorhynchus masou. Bull Jap Soc Sci Fish 50: 565- 571. https://doi.org/10.2331/suisan.50.565
  2. Arukwe A, Forlin L, Goksoyr A (1997) Xenobiotic and steroid biotransformation enzymes in Atlantic salmon (Salmo salar) liver treated with the estrogenic compound, 4-nonylphenol. Environ Toxicol Chem 16:2576-2583. https://doi.org/10.1002/etc.5620161220
  3. Beresford N, Routledge EJ, Harris CA, Sumpter JP (2000) Issues arising when interpreting results from an in vitro assay for estrogenic activity. Toxicol Appl Pharmacol 162: 22-33. https://doi.org/10.1006/taap.1999.8817
  4. Brion F, Tyler CR, Palazzi X, Laillet B, Porcher JM, Garric J, Flammarion P (2004) Impacts of 17$\beta$-estradiol, including environmentally relevant concentrations, on reproduction after exposure during embryo-larval-, juvenileand adult-life in zebrafish (Danio rerio). Aquat Toxicol 68: 193-217. https://doi.org/10.1016/j.aquatox.2004.01.022
  5. Cardinali M, Maradona F, Olivotto I, Bortoluzzi G, Mosconi G, Polzonetti-Magni AM, Carnevali O (2004) Temporary impairment of reproduction in freshwater teleost exposed to nonylphenol. Rep Toxicol 18:597-604. https://doi.org/10.1016/j.reprotox.2004.03.001
  6. Cavaco JEB, Santos CRA, Ingleton PM, Canario VM, Power DM (2003) Quantification of prolactin (PRL) and PRL receptor messenger RNA in gilthead seabream (Sparus aurata) after treatment with estradiol-17$\beta$. Biol Reprod 68:588-594. https://doi.org/10.1095/biolreprod.102.009209
  7. Christiansen T, Korsgaard B, Jespersen A (1998) Effects of nonylphenol and 17$\beta$-oestradiol on vitellogenin synthesis, testicular structure and cytology in male eelpout Zoarcesi iparus. J Exp Biol 201:179-192.
  8. Denny JS, Tapper MA, Schmieder, PK, Hornung MW, Jensen KM, Ankley GT, Henry TR (2005) Comparison of relative binding affinities of endocrine activity compounds to fathead minnow and rainbow trout estrogen receptors. Environ Toxicol Chem 24:2948-2953. https://doi.org/10.1897/04-595R.1
  9. Folmar LC, Gardner GR, Schreibman MP, Magliulo-Cepriano L, Mills LJ, Zaroogian G, Gutjahr-Gobell R, Haebler R, Horowitz DB, Denslow ND (2001) Vitellogenininduced pathology in male summer flounder (Paralichthys dentatus) Aquat Toxicol 51:431-441. https://doi.org/10.1016/S0166-445X(00)00121-1
  10. Gimeno S, Komen H, Gerritsen A, Bowmer T (1998) Feminization of young males of the common carp, Cyprinus carpio, exposed to 4-tert-pentylphenol during sexual differentiation. Aquat Toxicol 43:77-92. https://doi.org/10.1016/S0166-445X(98)00056-3
  11. Giesy JP, Pieren SL, Snyder EM, Miles-Richardson SM, Kramer VJ, Synder SA, Nichols KM, Villeneuve DL (2000) Effects of 4-nonylphenol on fecundity and biomarkers of estrogenicity in fathead minnows (Pimephales promelas). Environ Toxicol Chem 19:1368-1377. https://doi.org/10.1897/1551-5028(2000)019<1368:EONOFA>2.3.CO;2
  12. Gray MA, Metcalfe CD (1997) Induction of testis-ova in Japanese medaka (Oryzias latipes) exposed to p-nonylphenol. Environ Toxicol Chem 16:1082-1086.
  13. Hwang IJ, Baek HJ (2010) Effects of nonylphenol and 2,2",4,6,6"-pentachlorobiphenyl on in vitro steroidogenesis in maturing oocytes of ribbed gunnel, Dictyosoma burger. Dev Rprod 14(2):143-154
  14. Hern'andez-Rodriguez G, Zumbadoa M, Luzardo OP (2007) Multigenerational study of the hepatic effects exerted by the consumption of nonylphenol- and 4- octylphenol-contaminated drinking water in Sprague- Dawley rats. Environ Toxicol Pharmacol 23:73-81. https://doi.org/10.1016/j.etap.2006.07.004
  15. Inui M, Adachi T, Takenaka S, Inui H, Nakazawa M,Ueda M, Watanabe H, Mori C, Iguchi T, Miyatake K (2003) Effect of UV screens and preservatives on vitellogenin and choriogenin production in male medaka (Oryzias latipes). Toxicol 194: 43-50. https://doi.org/10.1016/S0300-483X(03)00340-8
  16. Jobling S, Sheanan D, Osborne JA, Matthiesen P, Sumpter JP (1996) Inhibition of testicular growth in rainbow trout (Oncorhynchus mykiss) exposed to alkylphenol chemicals. Environ Toxicol Chem 15:194-202. https://doi.org/10.1002/etc.5620150218
  17. Jung JH, Shim WJ, Addison RF, Baek JM, Han C.H (2006) Protein and gene expression of VTG in response to 4-nonylphenol in rockfish (Sebastes schlegeli). Comp Biochem Physiol C 143:162-170. https://doi.org/10.1016/j.cbpa.2005.11.008
  18. Kang IJ, Yokota H, Oshima Y, Tsuruda Y, Hano T, Maeda M, Imada N, Tadokoro H, Honjo T (2003) Effects of 4-nonylphenol on reproduction of Japanese medaka (Oryzias latipes). Environ Toxicol Chem 22:2438- 2445. https://doi.org/10.1897/02-225
  19. Kannan N, Yamashita N, Petrick G, Duinker JC (1998) Polychlorinated biphenyls (PCBs) and nonylphenols in the Sea of Japan. Environ Sci Technol 32:1747-1753. https://doi.org/10.1021/es970713q
  20. Kim DJ, Jung JH, An CM, Min GS, Gi YJ, Kim Y, Han CH (2006) Purification, partial characterization and immunoassay of vitellogenin from marbled sole (Limanda yokohamae). J Fish Sci Tech 8(4):213-219.
  21. Kime DE (1998) Endocrine Disruption in Fish. University of Sheffield Kluwer.
  22. Korsgaard B, Pedersen KL (1998) Vitellogenin in Zoarces viviparus: Purification, quantification by ELISA and induction by estradiol-17 beta and 4-nonylphenol. Comp Biochemist Physiol 120(1):159-166.
  23. Lech JJ, Lewis SK, Ren L (1996) In vivo estrogenic activity of nonylphenol in rainbow trout. Fund Appl Toxicol 30:229-232. https://doi.org/10.1006/faat.1996.0060
  24. Li D, Kim M, Oh JR, Park J (2004a) Distribution characteristics of nonylphenols in the artificial lake, Shihwa and surrounding creeks in Korea. Chemosphere 56: 783-790. https://doi.org/10.1016/j.chemosphere.2004.01.008
  25. Li D, Kim M, Dong M, Shim WJ, Hong SH, Oh JR, Yim UH, Jung JH, Kannan N, Kim ES, Cho SR (2004b) Seasonal and spatial distribution of nonylphenol and IBP in Saemangeum Bay, Korea. Mar Poll Bull 51: 966-974.
  26. Li Z, Li D, Oh JR, Je JG (2004) Seasonal and spatial distribution of nonylphenol in Shihwa lake, Korea. Chemosphere 56:611-618. https://doi.org/10.1016/j.chemosphere.2004.04.022
  27. Lomax DP, Roubal WT, Morre JD, Johnson LL (1998) An enzyme linked immunosorbent assay (ELISA) for measuring vitellogenin in English sole (P. vetulus): development, validation and cross-reactivity with other fleuronectids. Comp Biochem Physiol B 121:425-436. https://doi.org/10.1016/S0305-0491(98)10125-6
  28. Luo Q, Ban M, Ando H, Kitahashi T, Kumar Bhandari R, McCormick SD, Urano A (2005) Distinct effects of 4-nonylphenol and estrogen-17 beta on expression of estrogen receptor alpha gene in smolting sockeye salmon. Comp Biochem Physiol C 140:123-130.
  29. Matthews J, Celius T, Halgren R, Zacharewski T (2000) Differential estrogen receptor binding of estrogenic substances: A species comparison. J Steroid Biochem Mol Biol 74:223-234. https://doi.org/10.1016/S0960-0760(00)00126-6
  30. Mosconi G, Carnevali O, Carletta R, Nabissi M, Polzonetti- Magni AM (1998) Gilthead seabream (Sparus aurata) vitellogenin: Purification, partial characterization, and validation of an enzyme-linked immunosorbent assay (ELISA). Gen Comp Endocrinol 110:252-261. https://doi.org/10.1006/gcen.1998.7075
  31. Mollendorf A (1973) Cytology Cell Physiology, 3rd ed., Academic Press, New York.
  32. Nimrod AC, Benson WH (1997) Xenobiotic interaction with and alteration of channel catfish estrogen receptor. Toxicol Appl Pharmacol 147:381-390. https://doi.org/10.1006/taap.1997.8296
  33. Olsen CM, Meussen-Elholm ET, Hongslo JK, Stenersen J, Tollefsen KE (2005) Estrogenic effects of environmental chemicals: An interspecies comparison. Comp Biochem Physiol C 141:267-274. https://doi.org/10.1016/j.cbpc.2005.02.016
  34. Parks LG, Cheek AO, Denslow ND, Heppell SA, McLachlan JA, LeBlanc GA, Sullivan CV (1999) Fathead minnow (Pimephales promelas) VTG: purification, characterization and quantitative immunoassay for the detection of estrogenic compounds. Comp Biochem Physiol C 123:113-125. https://doi.org/10.1016/S1095-6433(99)00028-8
  35. Scholz S, Gutzeit HO (2000) 17$\beta$-ethylestradiol affects reproduction, sexual differentiation and aromatase gene expression of the medaka (Oryzias latipes). Aquat Toxicol 50:363-373. https://doi.org/10.1016/S0166-445X(00)00090-4
  36. Schwaiger J, Mallow U, Ferling H, Knoerr S, Braunbeck Th, Kalbfus W, Negele RD (2002) How estrogenic is nonylphenol? A transgenerational study using rainbow trout (Oncorhynchus mykiss) as a test organism. Toxicol 59: 177-189.
  37. Soverchia L, Ruggeri B, Palermo F, Mosconi G, Cardinaletti G, Scortichinic G, Gattic G, Polzonetti-Magni AM (2005) Modulation of vitellogenin synthesis through estrogen receptor beta-1 in goldfish (Carassius auratus) juveniles exposed to 17$\beta$-estradiol and nonylphenol. Toxicol Appl Pharmacol 209:236-243. https://doi.org/10.1016/j.taap.2005.04.013
  38. Sumpter JP, Jobling S (1995) Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment. Environ Health Perspect 103(Suppl. 7):173-178.
  39. Tollefsen KE, Meys JFA, Frydenlund J, Jørgen S (2002) Environmental estrogens interact with and modulate the properties of plasma sex steroid-binding proteins in juvenile Atlantic salmon (Salmo salar). Mar Environ Res 54:697-701. https://doi.org/10.1016/S0141-1136(02)00173-3
  40. Tollefsen, KE, Nilsen AJ (2007) Binding of alkylphenols and alkylated non-phenolics to rainbow trout (Oncorhynchus mykiss) hepatic estrogen receptors. Ecotoxicol Environ Saf 69(2):163-172.
  41. Van den Belt K, Verheyen R, Witters H (2003) Comparison of vitellogenin responses in zebrafish and rainbow trout following exposure to environmental estrogens. Ecotoxicol Environ Saf 56:271-281. https://doi.org/10.1016/S0147-6513(03)00004-6
  42. Villeneuve DL, Villalobos SA, Keith, TL, Snyder EM, Fitzgerald SD, Giesy JP (2002) Effects of waterborne exposure to 4-nonylphenol on plasma sex steroid and vitellogenin concentrations in sexually mature male carp (Cyprinus carpio). Chemosphere 47(1):15-28. https://doi.org/10.1016/S0045-6535(01)00212-0
  43. White R, Jobling S, Hoare SA, Sumpter JP, Parker MG (1994) Environmentally persistent alkylphenolic compounds are estrogenic. Endocrinol 135:175-182. https://doi.org/10.1210/en.135.1.175
  44. Yang FX, Ying X, Yang H (2006) Reproductive effects of prenatal exposure to nonylpenol on zebrafish (Danio rerio). Comp Biochem Physiol 142C:77-84.
  45. Yamaguchi A, Ishibashi H, Kohra S, Arizono K, Tominaga N (2005) Short-term effects of endocrine-disrupting chemicals on the expression of estrogen-responsive genes in male medaka (Oryzias latipes). Aquat Toxicol 72:239-249. https://doi.org/10.1016/j.aquatox.2004.12.011
  46. Zhang LY, Wang, CX (1984) Histopathological and histochemical studies on toxic effect of brodifacoum in mouse liver. Acta Acad Med Sci 6:386-388.