Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Evaluate a Repeated Oral Dose Toxicity and Immunomodulating Activity of $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ in Sprague-Dawley Rats

랫드에서 백두옹과 청호의 경구투여에 의한 반복 투여독성 시험과 면역 활성 평가

  • An, In-Jung (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kwon, Jung-Ki (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Jin-Seok (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Seung-Ho (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Young-Seok (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Byung-Kwon (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Sang-Ki (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Byeong-Soo (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Cho, Sung-Dae (School of dentistry, Institute of Oral Bioscience, Chonbuk National University) ;
  • Choi, Chang-Sun (School of Food Science and Technology, Chung-Ang University) ;
  • Lee, Byoung-Hee (National Institute of Biological Resources) ;
  • Kang, Byeong-Kon (Shinil Biogen) ;
  • Jung, Ji-Youn (Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University)
  • Received : 2011.11.22
  • Accepted : 2011.12.05
  • Published : 2012.03.31
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Abstract

This study was designed to evaluate a repeated oral dose toxicity and immunomodulating activity of $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ in Sprague-Dawley rats. The female rats were treated with $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ of control group, low group (0.5 ml/kg), medium group (1 ml/kg), high group (2 ml/kg) for distilled water, intragastrically for 4 weeks, respectively. To ensure the safety of $Pulsatilla$ $koreana $ and $Artemisiae$ $annuae$ such as the following were observed and tested. We examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the hematological and the serum biochemical analysis. We also observed the histopathological changes of liver and kidney in rats. Hematological results were the increase of neutrophils, lymphocytes and monocytes in the high dose group of $Pulsatilla$ $koreana$. The increase immune cells in the high dose group of $Pulsatilla$ $koreana$ might immunomodulating activity. No significant differences in body weight, feed intake, serum biochemical analysis and histopathological between control and fed group were found. In conclusion, $Pulsatilla$ $koreana$ and $Artemisiae$ $annuae$ is physiologically safe and improve immunomodulating activity.

본 연구는 Sprague-Dawley 계통의 암컷 랫드에서 백두옹과 청호를 반복경구투여 독성평가와 면역 활성을 평가하기 위해서 시행하였다. 랫드에 처치한 물질은 백두옹과 청호를 0.5 ml/kg, 1 ml/kg, 2 ml/kg를 D.W에 용해시켜 4주 동안 경구투여를 하였다. 백두옹과 청호의 안전성을 확인하기 위해 다음과 같은 관찰 및 검사를 하였다. 검사항목으로는 체중과 사료 섭취량, 임상증상, 안과학적 검사, 혈액학적 검사, 혈청생화학적 검사를 관찰하였다. 또한 랫드에 간과 신장에서의 병리조직학적 변화를 관찰하였다. 백두옹 고용량군의 혈액학적 검사에서 호중구, 림프구, 단핵구의 증가가 관찰되었다. 이러한 증가는 면역세포의 증가로 백두옹 고용량군은 면역 활성이 일어났다고 사료된다. 체중, 사료섭취량, 혈청생화학적 검사, 병리조직학적 변화는 대조군과 비교시 유의적인 변화가 나타나지 않았다. 따라서 백두옹과 청호는 생리대사에 무해하며 면역 활성이 증가된 것으로 사료된다.

Keywords

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