Journal of the Korean Society of International Agriculture (한국국제농업개발학회지)

The Korean Society of International Agriculture (한국국제농업개발학회)
권호 표지

Agronomic Characteristics and Compositional Variation in Seed Storage Proteins of Rice (Oryza Sativa L., Cv. Sindongjin) Mutant Lines

신동진벼 돌연변이계통의 작물학적 특성과 저장단백질 조성변이

  • Yoon, Min-Young (Department of Plant Resources, College of Industrial Sciences, Kongju National University) ;
  • Cho, Yoo-Hyun (Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University) ;
  • Ma, Kyung-Ho (National Academy of Agricultural Science, RDA) ;
  • Lee, Jung-Ro (National Academy of Agricultural Science, RDA) ;
  • Lee, Sok-Young (National Academy of Agricultural Science, RDA) ;
  • Park, Yong-Jin (Department of Plant Resources, College of Industrial Sciences, Kongju National University)
  • 윤민영 (공주대학교 식물자원학과) ;
  • 조유현 (공주대학교 두과녹비자원연구센터) ;
  • 마경호 (농촌진흥청 국립농업과학원) ;
  • 이정로 (농촌진흥청 국립농업과학원) ;
  • 이석영 (농촌진흥청 국립농업과학원) ;
  • 박용진 (공주대학교 식물자원학과)
  • Published : 2012.12.31
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Abstract

To facilitate an active breeding strategy for rice quality improvement, conventional mutations were carried out by treating rice variety (Sindongjin) seeds with gamma ray (300 Gray) and its fertilized eggs with MNU (1mM). Mutants were selected on the basis of endosperm and grain shape. Main agronomic characteristics and seed storage proteins of the mutants were investigated in 55 lines. Agronomic characteristics of the mutants showed generally earlier heading dates, shorter culm length and ear length than that of the parents. Besides, grain length, grain width, and 1,000 grain weight were comparatively shorter or lower than that of the parents. In SDS-PAGE analysis, SM-22 of Opaque group and SM-34 of Giant embryo group showed high glutelin polypeptide concentration. SM-23 of Floury group, SM-26 of Shrunken group, and SM-31 of Sugary group exhibited low concentration of total protein with variations above 55kDa bands.

종실 돌연변이를 유기하여 미질개선을 위한 육종으로의 적극적인 활용을 목적으로 얻어진 신동진벼 돌연변이 계통의 작물학적 특성과 종실 저장단백질을 변이모본과 비교 분석을 검토하여 얻은 결과를 요약하면 다음과 같다. 1. 종실 변이계통의 작물학적 특성은 변이모본보다 생태특성의 출수기에서는 대부분 조생의 경향을 보였고, 간장 및 수장은 짧은 경향을 나타냈고, 또한 종실특성의 현미 길이, 현미폭 및 천립중에서도 변이모본보다 짧거나 낮은 정도를 나타냈다. 2. SDS-PAGE 분석결과 opaque 군의 SM-22와 giant embryo 군의 SM-34는 글루테린 폴리펩타이드에서 높은 농도를 보였고, floury 군의 SM-23, shrunken 군의 SM-26, sugary 군의 SM-31은 전단백질 농도패턴은 낮게 보이면서 55kDa 이상의 고분자 band에서 다양성을 나타냈다.

Keywords

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