Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Characterization of Arthrospira platensis Mutants Generated by UV-B Irradiation

자외선 조사에 의해 유도된 미세조류 Arthrospira platensis 변이주의 특성

  • Park, Hyun-Jin (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University) ;
  • Kim, Young-Hwa (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
  • 박현진 (신라대학교 의생명과학대학 생명공학과) ;
  • 김영화 (신라대학교 의생명과학대학 제약공학과) ;
  • 이재화 (신라대학교 의생명과학대학 생명공학과)
  • Published : 2012.10.10
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Abstract

Arthrospira platensis (A. platensis) is an economically important filamentous microalgae. The isolation of mutants by chemical or physical mutagen is a clue for the strain improvement. In this study, effects of ultraviolet-B (UV-B) radiation on A. platensis were investigated. Cells (or microalgae) were exposed to UV-B (15 Watt, 254 nm) for 1, 3, 5, and 10 min, and resulting mutants were designated UM1, UM3, UM5, and UM10, respectively. Especially, the lipid content of UM5 mutant was considerably increased by 8~11 fold compared to that of wild types. Moreover, the carotenoid content and antioxidant enzyme (peroxidase and superoxide dismutase) activity were increased. It was shown that UV-induced mutants can accumulate bioactive compounds, which will be useful for the industrial production of valuable products.

Arthrospira platensis (A. platensis)는 상업적으로 중요한 사상형 미세조류이다. 화학적 혹은 물리적 돌연변이원에 의해 유도된 변이주 분리는 균주 개량에 중요하게 작용한다. 본 연구에서, A. platensis에서 자외선(UV-B)조사에 따른 영향을 살펴보았고, 그에 따른 변이주를 확보하였다. A. platensis를 자외선(15 Watt, 254 nm)을 이용하여 1, 3, 5, 10 min 동안 처리 한 후, 얻어진 변이주를 각각 UM1, UM3, UM5, UM10으로 명명하였다. 특히, UM5 변이주는 야생균주와 비교하여 지질의 함량이 8~11배 가량 크게 증가하였다. 또한, 카로티노이드 함량과 항산화 효소(peroxidase, superoxide dismutase) 활성이 증가하였다. 이 결과, 자외선에 의해 유도된 변이주는 생리활성 물질을 축적하였으며, 이러한 유용성분을 생산하는 미세조류의 균주 개발은 미세조류의 산업화를 촉진시킬 것으로 기대된다.

Keywords

References

  1. S. J. Dong, K. W. Kim, and S. Y. Cho, Kor. J. Fish. Aquat. Sci., 44, 1 (2011).
  2. H. M. Oh, A. R. Choi, and T. I. Mheen, Kor. J. Microbiol. Biotechnol., 31, 95 (2003).
  3. G. G. Choi, M. S. Bae, J. S. Park, B. J. Park, C. Y. Ahn, and H. M. Oh, Kor. J. Microbiol. Biotechnol., 35, 45 (2007).
  4. S. H. Cha, M. J. Kim, H. Y. Yang, C. B. Jin, Y. J. Jeon, T. Oda, and D. K. Kim, Kor. J .Fish Aquat Sci., 43, 437 (2010).
  5. B. H. Jo and H. J. Cha, KSBB J., 25, 109 (2010).
  6. P. Spolaore, C. J. Cassan, E. Duran, and A. Isambert, J. Biosci. Bioeng., 1, 87 (2006).
  7. K. D. Sung, J. H. Ann, J. Y. Lee, S. J. Ohh, and H. Y. Lee, Kor. J. Biotechnol. Bioeng., 10, 401 (1995).
  8. B. J. Park, B. M. Kim, S. H. Shim, J. D. Kim, and C. G. Lee, Kor. J. Microbiol. Biotechnol., 34, 136 (2006).
  9. C. Yoo, C. J. Kim, G. G. Choi, C. Y. Ahn, J. S. Choi, and H. M. Oh, Microbiol. Soc. Kor., 45, 268 (2009).
  10. J. C. Schneider, A. Livne, A. Sukenik, and P. G. Roessler, Cell culture biotechnol., 40, 807 (1995).
  11. R. Chaturvedi, S. R. Uppalapati, M. A. Alamsjah, and Y. Fujita, J. Appl. Phycol., 16, 135 (2004). https://doi.org/10.1023/B:JAPH.0000044826.70360.8e
  12. B. S. Kamath, R. Vidhyavathi, R. Sarada, and G. A. Ravishankar, Bioresour. Technol., 99, 8667 (2008). https://doi.org/10.1016/j.biortech.2008.04.013
  13. B. F. Cordero, I. Obraztsova, I. Couso, R. Leon, M. A. Vargas, and H. Rodriguez, Mar. Drugs, 9, 1607 (2011). https://doi.org/10.3390/md9091607
  14. T. J. Han, Kor. J. Environ. Biol., 17, 1 (1999).
  15. Y. M. Kim, M. R. Kim, T. H. Kwon, J. M. Ha, and J. H. Lee, J. Kor. Ind. Eng. Chem., 20, 285 (2009).
  16. J. Y. Kim, H. Joo, and J. H. Lee, Appl. Chem. Eng., 22, 301 (2011).
  17. W. Chen, M. Sommerfeld, and Q. Hu, Bioresour. Technol., 102, 135 (2011). https://doi.org/10.1016/j.biortech.2010.06.076
  18. S. I. Lee, J. Y. Park, J. G. Jung, D. G. Lee, S. H. Lee, J. M. Ha, B. J. Ha, and J. H. Lee, J. Life Sci., 15, 847 (2005). https://doi.org/10.5352/JLS.2005.15.6.847
  19. A. H. Teramura, L. H. Ziska, and A. E. Sztein, Physiol. Plant., 83, 373 (1991). https://doi.org/10.1111/j.1399-3054.1991.tb00108.x
  20. S. C. Singh, R. P. Sinha, and D. P. Hader, Acta Protozool., 41, 297 (2002).
  21. J. N. Rosenberg, G. A. Oyler, L. Wilkinson, and M. J. Betenbaugh, Curr. Op. Biotechnol., 19, 430 (2008). https://doi.org/10.1016/j.copbio.2008.07.008
  22. S. Boussiba, Physiol Plant., 108, 111 (2000). https://doi.org/10.1034/j.1399-3054.2000.108002111.x