Journal of People, Plants, and Environment (인간식물환경학회지)

Society For People, Plants, And Environment (인간식물환경학회)
권호 표지

Protection of Chenopodium album var. centrorubrum Extract by Oxidative Effect in Cultured Human Skin Fibroblasts Damaged by Cadmium

명아주 추출물의 항산화 효과에 의한 배양 인체피부섬유모세포에 대한 카드뮴 손상방어

  • Son, Young-Woo (Sanbon Medical Center, School of Medicine, Wonkwang University) ;
  • Choi, Yu-Sun (Sanbon Medical Center, School of Medicine, Wonkwang University)
  • 손영우 (원광대학교 의과대학 산본병원) ;
  • 최유선 (원광대학교 의과대학 산본병원)
  • Published : 2012.06.25
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Abstract

To evaluate the cytotoxicity of cadmium(Cd), an environmental pollutant, and the protective effect of Chenopodium album var. centrorubrum(CAC) extract on the Cd-induced cytotoxicity in cultured human skin fibroblasts(Detroit 551). Cultured Detroit 551 cells were treated or pretreated with cadmiun chloride($CdCl_2$) or CAC extract. In this study, $CdCl_2$ significantly decreased cell viability compared with control, and $XTT_{50}$ or $XTT_{50}$ value(midcytotoxicity value, MCV) was determined at a concentration of $41.1{\mu}M$ or $43.1{\mu}M$. The MCV of $CdCl_2$ was highly toxic by the criteria of Borenfreund and Puerner. While, $CdCl_2$-induced cytotoxicity was prevented by superoxide dismutase(SOD), an antioxidative enzyme. In the protective effect of CAC extract on $CdCl_2$-induced cytotoxicity, CAC extract prevented $CdCl_2$-induced cytotoxicity by the significant increase of cell viability decreased by $CdCl_2$-induced cytotoxicity. And also, CAC extract showed antioxidative effects such as electron donating activity(EDA) and inhibitory activity of lipid peroxidation(LP). From above the results, it is suggested that $CdCl_2$ was highly toxic to cultured Detroit 551 cells and CAC extract was effective in the blocking $CdCl_2$-induced cytotoxicity by the antioxidative effects such as EDA and inhibitory activity of LP. In conclusion, the physical activity of natural component such as CAC extract may be a useful resource for preventing diseases mediated by reactive oxygen species(ROS) such as heavy metal toxication, stroke or dementia.

환경오염원이면서 중금속의 일종인 카드뮴(cadmium)의 세포독성과 이에 대한 명아주(Chenopodium album var. centrorubrum, CAC) 추출물의 영향을 배양 인체피부섬유모세포(Detroit 551)를 재료로 항산화 측면에서 조사하여 다음과 같은 결과를 얻었다. Cadmium chloride($CdCl_2$)는 농도 의존적으로 배양 Detroit 551 세포의 세포생존율(cell viability, CV)과 세포부착률(cell adhesion activity, CAA)을 유의하게 감소시킴으로써 세포독성을 나타냈다. 한편, 항산화제의 일종인 superoxide dismutase(SOD)는 $CdCl_2$에 의해 감소된 CAA를 유의하게 증가시킴으로써 $CdCl_2$에 의한 세포독성을 방어하였다. 한편, $CdCl_2$의 세포독성에 대한 CAC 추출물의 영향에 있어서, CAC 추출물은 $CdCl_2$에 의하여 감소된 CAA를 유의하게 증가시킴으로써 $CdCl_2$에 의한 세포독성을 방어하였으며, 또한 전자공여능(electron donating activity, EDA)과 지질과산화(lipid peroxidation, LP) 억제능을 나타냈다. 이상의 결과에서 $CdCl_2$의 세포독성에 산화적 손상이 관여하고 있으며 CAC 추출물은 $CdCl_2$에 의하여 감소된 CV 및 CAA의 증가를 비롯하여, EDA 및 지질과산화 억제능과 같은 항산화 효과(antioxidative effect)에 의하여 세포독성을 방어한 것으로 나타났다. 따라서 CAC와 같은 식물추출물 성분에 대한 항산화 측면에서의 생리활성 구명은 산화적 손상으로 매개되는 중금속 중독을 비롯한 뇌졸중 및 치매와 같은 난치성 질환의 치료적 접근을 위한 소재로서의 활용성이 클 것으로 생각된다.

Keywords

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