The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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A Study on Inhibitory Mechanism of Melia Fructus Extract on Osteoclast Differentiation

천연자(川楝子)의 파골세포 분화 억제기전 연구

  • Yun, Young-Jin (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Jin-Moo (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Chang-Hoon (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Jang, Jun-Bock (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Kyung-Sub (Dept. of Oriental Gynecology, College of Oriental Medicine, Kyung-Hee University)
  • 윤영진 (경희대학교 한의과대학 한방부인과교실) ;
  • 이진무 (경희대학교 한의과대학 한방부인과교실) ;
  • 이창훈 (경희대학교 한의과대학 한방부인과교실) ;
  • 장준복 (경희대학교 한의과대학 한방부인과교실) ;
  • 이경섭 (경희대학교 한의과대학 한방부인과교실)
  • Received : 2012.04.27
  • Accepted : 2012.05.15
  • Published : 2012.05.25
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Abstract

Objectives: This study was conducted to evaluate the inhibitory effect of Melia Fructus extract on osteoclast differentiation. Methods: MTT-assay was performed to estimate cytotoxicity of Melia Fructus extract in BMMs stimulated with M-CSF. TRAP staining, TRAP activity and Real-time PCR were performed to know the inhibitory effect on osteoclast differentiation. Actin ring formation were analysed to observe the effect of Melia Fructus extract. Results: Melia Fructus extract decreased the number of TRAP positive cells and the expression of NFATc1 gene, c-Fos gene, TRAP and OSCAR in BMMs stimulated with RANKL. Melia Fructus extract has no cytotoxicity at the concentration used in this study. Melia Fructus extract restrained the formation of actin ring. Melia Fructus inhibited NF-${\kappa}B$ activity by inducing degradation of p-$IkB{\alpha}$. Conclusions: Melia Fructus has the inhibitory effect of osteocalst differentiation and bone resorption. Further studies are needed to treat osteoporosis by herbal medicine containing Melia Fructus.

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References

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