Abstract
With the addition of ethanol to wax gourd extract and by acetic fermentation, 5.0% acidity vinegar was produced. After putting 10% extract(10% concentration) of Chrysanthemum zawadskii in this, and by dissolving shell, Chrysanthemum zawadskii-pearl vinegar was produced. When a 1% of ark shell, oyster shell, or ear shell was added to wax gourd vinegar, 95.6~98.4% of the shell dissolved, and when a 2% content of shell was added, 97.2~98.4% was dissolved. The acidity of vinegar which dissolved 1% shell was pH 3.0~3.17, and the acidity of vinegar which dissolved 2% shell was pH 1.11~ 1.20. The pH values of vinegar which dissolved 1%, and 2% shell contents were 4.54~4.55, and 4.86~4.95, respectively. When 1% shell was dissolved, the acidity was higher than that of commercial vinegar, with a high pH value and low level of free acid. This shows that when Chrysanthemum zawadskii 1% is added during acetic acid fermentation, the inhibition was 44.4%, and 22.2% respectively. In this regard, Chrysanthemum zawadskii should be added after the fermentation of acetic acid. The calcium content of 1% shell vinegar is 0.4%, and that of 2% vinegar is 0.78%. Non-heated native wax gourd shows an angiotensin converting enzyme inhibition rate of 21.7%, an antioxidant activity of 5.23%, and a tyrosinase inhibition rate of 5.5%. In the case of heated-extracted wax gourd, the angiotensin converting enzyme inhibition rate was 16.1%, superoxide dismutase activity was 20.5%, antioxidant activity was 23.2%, and the tyrosinase inhibition rate was 7.1%. Also, in the case of Chrysanthemum zawadskii, the angiotensin converting enzyme inhibition rate was 28.8%, the xanthine oxidase inhibition rate was 28.2%, the superoxide dismutase activity was 14.5%, the antioxidant activity was 3.2%, and the tyrosinase inhibition rate was 9.2% Data also revealed that when a 10% sample of the heated-wax gourd extract was added to A549 human lung cancer epithelial cells of, the number of cancer cells declined by 80% in 72 hours, When a 10% native extract was added, the number of cells declined by, 74% in 48 hours, and when a heated-extract of Chrysanthemum zawadskii was added, 100% of the cells died after 72 hours.
동과착즙액에 에탄올을 가해 초산발효시켜서 산도 5.0%의 식초를 만들고, 여기에 구절초 추출액(농도 10%)을 10%를 가한 다음, 조개껍질을 녹여서 식초를 제조하였다. 동과 식초에 대하여 꼬막껍질, 굴껍질, 전복껍질을 1% 가하면 97.2~98.4% 녹고, 2% 가하면 95.8~95.6% 녹았다. 조개껍질을 1% 녹인 식초의 산도는 3.00~3.17, 2% 녹인 것은 1.11~1.20였고, 조개껍질을 1% 녹인 식초의 pH는 4.54~4.55, 2% 녹인 식초는 4.86~4.95로 조개껍질 1%짜리의 경우 시판 식초보다 산도가 높으면서도 pH 수치가 높아서 유리산이 적었다. 초산발효시 구절초를 가하면 1%일 경우 저해율 44.4%, 0.12%일 경우 저해율 22.2%을 나타내므로 구절초는 초산발효 후 첨가해야 한다. 조개껍질 1% 식초의 칼슘함량은 0.4%, 2% 식초는 0.78%였다. 열처리하지 않은 동과는 angiotensin 전환효소 저해활성 21.7%, 항산화효소 활성 5.23%, 티로시나제 저해활성 5.5%를 나타냈고, 열처리 추출 동과는 angiotensin 전환효소 저해활성 16.1%, superoxide dismutase 활성 20.5%, 항산화 활성 23.2%, 티로시나제 저해활성 7.1%를 나타냈다. 구절초는 angiotensin 전환효소 저해활성 28.8%, xanthine oxidase 저해활성 28.2%, speroxide dismutase 활성 14.5%, 항산화 활성 3.2%, 티로시나제 저해활성 9.2%로 나타났다. 10% 시료를 인간폐암상피세포주 A549 세포에 가한 결과, 세포는 동과가열추출액의 경우, 72시간에 80%, 생동과는 48시간 뒤에 74%, 구절초 가열 추출액은 72시간 뒤에 100% 암세포를 감소시켜 항암작용을 나타냈다.
