The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Diversity of Endophytic Fungi from the Roots of Halophytes Growing in Go-chang Salt Marsh

고창갯벌의 염생식물 뿌리로부터 분리된 내생진균의 다양성

  • You, Young-Hyun (School of Life Sciences, Kyungpook National University) ;
  • Yoon, Hyeok-Jun (School of Life Sciences, Kyungpook National University) ;
  • Woo, Ju-Ri (School of Life Sciences, Kyungpook National University) ;
  • Seo, Yeong-Gyo (School of Life Sciences, Kyungpook National University) ;
  • Kim, Mi-Ae (School of Life Sciences, Kyungpook National University) ;
  • Lee, Gyeong-Min (School of Life Sciences, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Sciences, Kyungpook National University)
  • Received : 2012.05.31
  • Accepted : 2012.06.20
  • Published : 2012.06.30
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Abstract

Halophyte samples, such as Suaeda japonica, Phragmites australis, Limonium tetragonum, Suaeda maritima were collected from Go-chang salt marsh. Thirty-nine endophytic fungal strains were isolated from the roots of halophytes naturally growing in salt marsh. All endophytic fungal strains isolated were analyzed by internal transcribed spacer (ITS) containing ITS1, 5.8 s and ITS2 region. Endophytic fungal strains belong to eight orders, i.e., Eurotiales (36%), Pleosporales (26%), Hypocreales (18%), Incertae sedis (8%), Glomerellales (5%), Sordariales (2%), Xylariales (2%), and Capnodiales (3%). On genus level, they were composed of Alternaria, Aspergillus, Chaetomium, Cladosporium, Colletotrichum, Coniothyrium, Dothideomycete, Fusarium, Gibberella, Macrophoma, Penicillium, Pestalotiopsis, Phaeosphaeria, Phoma, Pleosporales, Pseudozyma, Talaromyces, and Termitomyces. Of them, Penicillium (26%), Fusarium (13%) of Eurotiales and Hypocreales were predominant.

염생식물인 칠면초, 갈대, 갯질경, 해홍나물을 고창갯벌로부터 채집하였으며, 39점의 내생진균을 염 습지에 자생하고 있는 염생식물의 뿌리로부터 분리하였다. 내생진균들은 ITS1, 5.8s와 ITS2를 포함하는 ITS-rDNA 영역에 의해 분석되었다. 다양한 내생진균은 Eurotiales (36%), Pleosporales (26%), Hypocreales (18%), Incertae sedis (8%), Glomerellales (5%), Sordariales (2%), Xylariales (2%), Capnodiales (3%)등 8종류 목에 속하는 것으로 확인되었다. 39점의 내생진균은 속(genus)단계에서 Alternaria, Aspergillus, Chaetomium, Cladosporium, Colletotrichum, Coniothyrium, Dothideomycete, Fusarium, Gibberella, Macrophoma, Penicillium, Pestalotiopsis, Phaeosphaeria, Phoma, Pleosporales, Pseudozyma, Talaromyces, 그리고 Termitomyces에 포함되는 것으로 분석되었다. 그리고 내생진균은 Eurotiales목의 Penicillium속이 가장 많이 분포하고 있는 것으로 분석되었다.

Keywords

References

  1. Arnold, E. A., Mejia, L. C., Kyllo, D., Rojas, E., Maynard, Z., Robbins, N. and Herre, E. A. 2003. Fungal endophytes limit pathogen damage in a tropical tree. PNAS 100:15649-15654. https://doi.org/10.1073/pnas.2533483100
  2. Chapman, V. J. 1974. Salt marshes and salt deserts of the world. In ecology of halophytes. Academic Press, pp.3-22. New York. USA.
  3. Ding, B., Yin, Y., Zhang, F. and Li, Z. 2011. Recovery and phylogenetic diversity of culturable fungi associated with marine sponges Clathrina luteoculcitella and Holoxea sp. in the South China Sea. Mar. Biotechnol. 13:713-21. https://doi.org/10.1007/s10126-010-9333-8
  4. Gomes, N. C. M., Cleary, D. F. R., Pinto, F. N., Egas, C., Almeida, A., Cunha, A., Mendona-Hagler, L. C. S. and Smalla, K. 2010. Taking root: enduring effect of rhizosphere bacterial colonization in mangroves. PLoS ONE 5, e14065. https://doi.org/10.1371/journal.pone.0014065
  5. Hamayun, M., Khan, S. A., Khan, M. A., Khan, A. L., Kang, S. M., Kim, S. K., Joo, G. J. and Lee, I. J. 2009. Gibberellin production by pure cultures of a new strain of Aspergillus fumigates. World J. Microbiol. Biotechnol. 25:1785-1792. https://doi.org/10.1007/s11274-009-0078-3
  6. Khan, A. L., Hamayun, M., Ahmad, N., Hussain, J., Kang, S. M., Kim, Y. H., Adnan, M., Tang, D. S., Waqas, M., Radhakrishnan, R., Hwang, Y. H. and Lee, I. J. 2011. Salinity stress resistance offered by endophytic fungal interaction between Penicillium minioluteum LHL09 and Glycine max. L. J Microbiol. Biotechnol. 21:893-902. https://doi.org/10.4014/jmb.1103.03012
  7. Khan, A. L., Hamayun, M., Kang, S. M., Kim, Y. H., Jung, H. Y., Lee, J. H. and Lee, I. J. 2012. Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: an example of Paecilomyces formosus LHL10. BMC Microbiol. 12:3.
  8. Kim, B. S., Oh, H. M., Kang, H., Park, S. and Chun. J. 2004. Remarkable bacterial diversity in the tidal flat sediment as revealed by 16S rDNA analysis. J. Microbiol. Biotechnol. 14:205-211.
  9. Khan, S. A., Hamayun, M., Rim, S. O., Lee, I. J., Seu, J. C., Choo, Y. S., Jin, I. N., Kim, S. D., Lee, I. K. and Kim, J. G. 2008. Isolation of endophytic fungi capable of plant growth promotion from monocots inhibited in the coastal sand dunes of Korea. Korean J. Life Sci. 18:1355-1359. https://doi.org/10.5352/JLS.2008.18.10.1355
  10. Khan, S. A., Hamayun, M., Kim, H. Y., Yoon, H. J., Lee, I. J. and Kim, J. G. 2009. Gibberellin production and plant growth promotion by a newly isolated strain of Gliomastix murorum. World J. Microbiol. Biotechnol. 25:829-833. https://doi.org/10.1007/s11274-009-9981-x
  11. Kil, Y. J., Eo, J. K. and Eom, A. H. 2009. Molecular identification and diveristy of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Korean J. Mycol. 37:130-133. https://doi.org/10.4489/KJM.2009.37.2.130
  12. Mohamed, D. J. and Martiny, J. B. 2011. Patterns of fungal diversity and composition along a salinity gradient. ISME. J. 5:379-388. https://doi.org/10.1038/ismej.2010.137
  13. Park, K. H. and Lee, H. K. 2006. Establishment of the wetland ecosystem information system based on Web-GIS in Gyeongnam region. J. Environ. Res. 6:99-103
  14. Pielou, E. C. 1975. Ecological diversity. John Wiley, p 165. New York, USA.
  15. Redman, R. S., Sheehan, K. B., Stout, R. G., Rodriguez, R. J. and Henson, J. M. 2002. Thermotolerance conferred to plant host and fungal endophyte during mutualistic symbiosis. Science 298:1581. https://doi.org/10.1126/science.1072191
  16. Rodriguez, R. J., Redman, R. S. and Henson, J. M. 2004. The role of fungal symbioses in the adaptation of plants to high stress environments. Mitigation and Adaptation Strategies for Global Change. 9:261-272. https://doi.org/10.1023/B:MITI.0000029922.31110.97
  17. Rodriguez, R. J., Henson, J., Van, V. E., Hoy, M., Wright, L., Beckwith, F., Kim, Y. and Redman, R. S. 2008. Stress tolerance in plants via habitat-adapted symbiosis. ISME J. 2:404-416. https://doi.org/10.1038/ismej.2007.106
  18. Seo, S. T., Kim, K. H., Kim, M. J., Hong, J. S., Park, J. H. and Shin, S. C. 2009. Diversity of fungal endophytes from Pinus koraiensis leaves in Korea. Korean J. Mycol. 37:108-110. https://doi.org/10.4489/KJM.2009.37.1.108
  19. Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Bio. Evol. 24:1596-1599. https://doi.org/10.1093/molbev/msm092
  20. Vazquez M. M., Cesar, S. Azcon, R. and Barea, J. M. 2000. Interaction between arbuscular mycorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities in the rhizosphere of maize plants. Appl. Soil Ecol. 15:261-272. https://doi.org/10.1016/S0929-1393(00)00075-5
  21. Waller, F., Achatz, B., Baltruscha, T. H., Fodor, J., Becker, K., Fischer, M., Heier, T., Hckelhoven, R., Neumann, C., Wettstein, D. V., Franken, P. and Kogel, K. H. 2005. The endophytic fungus Piriformospora indica reprograms barley to saltstress tolerance, disease resistance, and higher yield. PNAS. 102:13386-13391. https://doi.org/10.1073/pnas.0504423102
  22. Yamada, A., Takeo, O., Yosuke, D. and Masatake, O. 2001. Isolation of Tricholoma matsutake and T. bakamatsutake cultures from field-collected ectomycorrhizas. Mycoscience. 42:43-50. https://doi.org/10.1007/BF02463974
  23. You, Y. H., Yoon, H., Lee, G. S., Woo, J. R., Shin, J. H., Lee, I. J., Rim, S. O., Choo, Y. S. and Kim, J. G. 2011. Diversity and plant growth-promotion of endophytic fungi isolated from the roots of plants in Dokdo islands. Korean J. Life Sci. 21:992-996. https://doi.org/10.5352/JLS.2011.21.7.992
  24. Zhang, X. Y., Bao, J., Wang, G. H., He, F., Xu, X. Y. and Qi, S. H. 2012. Diversity and antimicrobial activity of culturable fungi isolated from six species of the South China sea gorgonians. Microb. Ecol. In press. DOI 10.1007/s00248-012-0050-x.

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