Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Characterization of the Variability of Nucleoli in the Cells of Panax ginseng Meyer In Vivo and In Vitro

  • Khrolenko, Yuliya A. (Institute of Biology and Soil Science, Far Eastern Branch of Russian Academy of Science) ;
  • Burundukova, Olga L. (Institute of Biology and Soil Science, Far Eastern Branch of Russian Academy of Science) ;
  • Lauve, Lyudmila S. (Institute of Biology and Soil Science, Far Eastern Branch of Russian Academy of Science) ;
  • Muzarok, Tamara I. (Institute of Biology and Soil Science, Far Eastern Branch of Russian Academy of Science) ;
  • Makhan'kov, Vyacheslav V. (Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Science) ;
  • Zhuravlev, Yuri N. (Institute of Biology and Soil Science, Far Eastern Branch of Russian Academy of Science)
  • Received : 2010.10.15
  • Accepted : 2011.06.20
  • Published : 2012.07.15
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Abstract

Results of karyological study of intact plants and some callus lines of Panax ginseng are presented. In the native plants of P. ginseng the nucleus with 1 nucleolus (90%) dominate, and nucleus with 2 nucleoli is rare. One nucleolar nucleus also dominate in interphase nuclei of cells of cultivated P. ginseng (from 2006), but we also found nucleus with 2 to 3 nucleoli in the same cell lines. Interphase nuclei of P. ginseng in long cultivated lines (from 1988) contain 1 to 9 nucleoli, with a predominance of nuclei containing from 3 to 4 nucleoli. It was shown that long-time cells (cultivated since 1988) had cytogenetic changes such as increase level of polyploid and aneuploid cells, increase of nucleoli number into interphase nucleus and decrease of nuclei/nucleoli ratio. These long-time cultivated cells had very low ginsenoside content.

Keywords

References

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