The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Development of Estimation Methods of Skin Oxidation and Evaluation of Anti-Oxidative Effects of Genistein in Topical Formulations

  • Kim, Seong-Yeon (College of Pharmacy, Chung-Ang University) ;
  • Na, Yeon-Joo (College of Pharmacy, Chung-Ang University) ;
  • Kim, Dong-Ju (College of Pharmacy, Chung-Ang University) ;
  • Kim, Yeong-Seok (College of Pharmacy, Chung-Ang University) ;
  • Kim, Hyeong-Min (College of Pharmacy, Chung-Ang University) ;
  • Hwang, Sung-Ha (College of Pharmacy, Chung-Ang University) ;
  • Kwak, Ji-Yeon (Department of Physiology and Biophysics, Inha University College of Medicine) ;
  • Kuh, Hyo-Jeong (Department of Biomedical Science, College of Medicine, The Catholic University of Korea) ;
  • Lee, Jae-Hwi (College of Pharmacy, Chung-Ang University)
  • Received : 2012.04.03
  • Accepted : 2012.05.30
  • Published : 2012.06.30
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Abstract

The objective of the present study was to establish the method of measurement of hydrogen peroxide and to estimate the anti-oxidative effect of genistein in the skin. UVB induced skin oxidation and anti-oxidative effect of genistein formulations were evaluated by determining levels of hydrogen peroxide. The mechanism involved in the determination of hydrogen peroxide is based on a color reaction between ferric ion ($Fe^{3+}$) and xylenol orange, often called FOX assay and subsequent monitoring of absorbance values of the reactant at 540 nm. The reaction was to some extent pH-dependent and detection sensitivity was greatest at pH 1.75. Genistein liposomal gel demonstrated better anti-oxidative effect with regard to lowering hydrogen peroxide levels elevated by UVB irradiation compared to genistein-suspended gel. A linear relationship has been observed between anti-oxidative effect of genistein and drug deposition in the skin tissue. Genistein liposomal gel resulting in the localization of the drug in the deeper skin led to improved anti-oxidative effect compared to genistein gel. The suggested method for evaluation of oxidation of the skin can be used as a tool to screen effective anti-oxidative agents and their delivery systems acting on the skin.

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References

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