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Effects of Black Garlic Supplementation and Exercise on TBARS, HSP 70 and COX-2 Expression after High-intensity Exercise

흑마늘 투여와 운동이 고강도 운동 후 TBARS, HSP 70 및 COX-2 발현에 미치는 영향

  • Baek, Yeong-Ho (Department of Physical Education, Pusan National University) ;
  • Lee, Sang-Ho (Department of Physical Education, Pusan National University) ;
  • Han, Min-Ho (Department of Biomaterial Control, Graduate School, Dong-Eui University) ;
  • Choi, Yung-Hyun (Department of Biomaterial Control, Graduate School, Dong-Eui University) ;
  • Kim, Seung-Hyun (Department of Physical Education, Pusan National University) ;
  • Kwak, Yi-Sub (Department of Physical Education, Dong-Eui University)
  • 백영호 (부산대학교 체육교육과) ;
  • 이상호 (부산대학교 체육교육과) ;
  • 한민호 (동의대학교 대학원 바이오물질제어학과) ;
  • 최영현 (동의대학교 대학원 바이오물질제어학과) ;
  • 김승현 (부산대학교 체육교육과) ;
  • 곽이섭 (동의대학교 체육학과)
  • Received : 2012.03.13
  • Accepted : 2012.05.07
  • Published : 2012.06.30

Abstract

The purpose of this study was to investigate the effects of black garlic supplementation and exercise on TBARS, HSP 70, and COX-2 expression after high-intensity exercise in rats. Twenty-four male Sprague-Dawley rats (4 weeks) were used in this study. Experimental groups were a control group (A, n=6), black garlic supplementation group (B, n=6), exercise group (C, n=6), and black garlic supplementation with exercise group (D, n=6). High-intensity exercise was performed by treadmill running (every 10 min speed increased gradually; 15~24 m/min, $0^{\circ}$ grade) and 2.86 g/kg black garlic for 4 weeks. The findings of this study were as follows: TBARS levels were significantly lower in the D group compared to the other group and B and C groups were significantly lower in TBARS levels than in the A group. HSP 70 expression was lower in the B group compared to the other groups. COX-2 expression has showed a similar expression in all groups. Therefore, it is considered that black garlic intake and exercise may reduce oxidative stress.

실험동물은 생후 4주령 Sprague Dawley계 수컷 흰쥐 24마리로 흑마늘운동군(6마리), 운동군(6마리), 흑마늘군(6마리), 대조군(6마리)으로 총 24마리로 구분하여 운동은 총 4주간 트레드밀로 실시하였으며, 시료 채취 하기 바로 직전에 고강도 운동을 실시하였다. 흑마늘 섭취는 흑마늘진액을 구강 투여시켰으며, 연구결과 다음과 같다. 4주간의 흑마늘 투여와 유산소운동 후 고강도 운동을 실시하였을 때 TBARS 농도는 흑마늘운동군이 흑마늘군, 운동군과 대조군에 비해 유의하게 낮았다. 흑마늘군과 운동군은 대조군에 비해 유의하게 낮았다. HSP 70 발현은 흑마늘섭취군이 다른군에 비해 상대적으로 낮게 나타났다. COX-2 발현은 모든군에서 비슷한 발현을 보이고 있으며 규칙적인 운동과 흑마늘 섭취는 산화적 스트레스를 줄일 수 있을 것으로 기대된다.

Keywords

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