Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Preparation and Properties of Collagen-Liposome using Hydrogenated Phosphatidylcholine

수첨 포스파티딜콜린을 이용한 콜라겐 리포좀의 제조 및 특성

  • Choi, Young-Won (Department of Engineering Chemistry, College of Engineering Chungbuk National University) ;
  • Jang, Boo-Sik (Department of Engineering Chemistry, College of Engineering Chungbuk National University) ;
  • Jeong, Noh-Hee (Department of Engineering Chemistry, College of Engineering Chungbuk National University)
  • 최영원 (충북대학교 공과대학 공업화학과) ;
  • 장부식 (충북대학교 공과대학 공업화학과) ;
  • 정노희 (충북대학교 공과대학 공업화학과)
  • Received : 2012.06.01
  • Accepted : 2012.06.26
  • Published : 2012.06.30
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Abstract

The collagen-liposomes were prepared with hydrogenated phosphatidylcholine and by solvent injection method for maximum protection and stable absorption of collagen into the body. Cholesterol was added to phosphatidylcholine to increase the stability of liposome membrane. The mixture was dissolved in ethanol and propylene glycol and PBS Buffer was used to Stabilize ions. The properties collagen-liposome were analyzed by DLS, SEM and POM.

콜라겐 성분을 최대로 보호하면서 안정하게 체내로 흡수 될 수 있도록 고순도 수첨 포스 파티딜콜린과 용매사출방법을 이용하여 콜라겐 리포좀을 제조하였다. 리포좀 막의 안정성을 높이기 위해 포스파티딜콜린에 콜레스테롤을 혼합하여, 에탄올과 프로필렌글리콜 혼합용매에 용해하였으며, 이온의 안정화를 위하여 PBS Buffer를 사용하였다. 다양한 변수에 의해 제조된 콜라겐 리포좀의 특성은 동적광산란광도계(DLS), 주사현미경(SEM), 편광현미경(POM)로 분석하였다.

Keywords

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