Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Triptolide Inhibits Proliferation and Induces Apoptosis of Human Melanoma A375 Cells

  • Tao, Yue (Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College) ;
  • Zhang, Meng-Li (Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College) ;
  • Ma, Peng-Cheng (Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College) ;
  • Sun, Jian-Fang (Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College) ;
  • Zhou, Wu-Qing (Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College) ;
  • Cao, Yu-Ping (Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College) ;
  • Li, Ling-Jun (Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College)
  • Published : 2012.04.30
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Abstract

Triptolide, a diterpenoid obtained from Tripteryglum wilfordii Hook.f, has attracted interest for its antitumor activities against human tumor cell lines in recent years. This report focuses on anti-proliferative and pro-apoptotic activities in human melanoma A375 cells assessed by CCK8 assay, Hoechst 33258 staining and flow cytometry. In addition, triptolide-induced arrest in the S phase was also observed. Caspase assays showed the apoptosis induced by triptolide was caspase-dependent and probably through intrinsic apoptotic pathways. Furthermore, expression of NF-${\kappa}B$ (p65) and its downstream factors such as Bcl-2, Bcl-$X_L$ was down-regulated. Taken together, the data indicate that triptolide inhibits A375 cells proliferation and induces apoptosis by a caspase-dependent pathway and through a NF-${\kappa}B$-mediated mechanism.

Keywords

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