Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Radiosensitivity Enhancement by Arsenic Trioxide in Conjunction with Hyperthermia in the EC-1 Esophageal Carcinoma Cell Line

  • Cui, Yan-Hui (Oncology Department, the First Affiliated Hospital of Xinxiang Medical College) ;
  • Liang, Hai-Jun (Infectious Disease I Department, the First Affiliated Hospital of Xinxiang Medical College) ;
  • Zhang, Qing-Qin (Oncology Department, the First Affiliated Hospital of Xinxiang Medical College) ;
  • Li, Si-Qing (Tuberculosis Department, the First Affiliated Hospital of Xinxiang Medical College) ;
  • Li, Xiao-Rui (Oncology Department, the First Affiliated Hospital of Xinxiang Medical College) ;
  • Huo, Xiao-Qing (Oncology Department, the First Affiliated Hospital of Xinxiang Medical College) ;
  • Yang, Qing-Hui (Oncology Department, the First Affiliated Hospital of Xinxiang Medical College) ;
  • Li, Wei-Wei (Oncology Department, the First Affiliated Hospital of Xinxiang Medical College) ;
  • Gu, Jian-Fa (The Fourth People Hospital of Zhengzhou) ;
  • Hua, Qin-Liang (Oncology Department, the First Affiliated Hospital of Xinxiang Medical College) ;
  • Lu, Ping (Oncology Department, the First Affiliated Hospital of Xinxiang Medical College) ;
  • Miao, Zhan-Hui (Oncology Department, the First Affiliated Hospital of Xinxiang Medical College)
  • Published : 2012.04.30
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Abstract

Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

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