Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Ramlibacter ginsenosidimutans sp. nov., with Ginsenoside-Converting Activity

  • Wang, Liang (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • An, Dong-Shan (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Kim, Song-Gun (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jin, Feng-Xie (College of Bio & Food Technology, Dalian Polytechnic University) ;
  • Kim, Sun-Chang (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Lee, Sung-Taik (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Im, Wan-Taek (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
  • Received : 2011.06.20
  • Accepted : 2011.11.09
  • Published : 2012.03.28
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Abstract

A novel ${\beta}$-proteobacterium, designated BXN5-$27^T$, was isolated from soil of a ginseng field of Baekdu Mountain in China, and was characterized using a polyphasic approach. The strain was Gram-staining-negative, aerobic, motile, non-spore-forming, and rod shaped. Strain BXN5-$27^T$ exhibited ${\beta}$-glucosidase activity that was responsible for its ability to transform ginsenoside $Rb_1$ (one of the dominant active components of ginseng) to compound Rd. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the family Comamonadaceae; it was most closely related to Ramlibacter henchirensis $TMB834^T$ and Ramlibacter tataouinensis$TTB310^T$ (96.4% and 96.3% similarity, respectively). The G+C content of the genomic DNA was 68.1%. The major menaquinone was Q-8. The major fatty acids were $C_{16:0}$, summed feature 4 (comprising $C_{16:1}$ ${\omega}7c$ and/or iso-$C_{15:0}$ 2OH), and $C_{17:0}$ cyclo. Genomic and chemotaxonomic data supported the affiliation of strain BXN5-$27^T$ to the genus Ramlibacter. However, physiological and biochemical tests differentiated it phenotypically from the other established species of Ramlibacter. Therefore, the isolate represents a novel species, for which the name Ramlibacter ginsenosidimutans sp. nov. is proposed, with the type strain being BXN5-$27^T$ (=DSM $23480^T$ = LMG $24525^T$ = KCTC $22276^T$).

Keywords

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