Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant Effect of Hot water and Ethanol extracts from Cheonnyuncho (Opuntia humifusa) on Reactive Oxygen Species (ROS) Production in 3T3-L1 Adipocytes

3T3-L1 지방세포내 ROS 생성에 대한 천년초 열수 및 에탄올 추출물의 항산화 효과

  • Yoon, Bo-Ra (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Young-Jun (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kim, Sun-Gu (Department of Food Science, Kongju National University) ;
  • Jang, Jung-Young (Department of Food Science, Kongju National University) ;
  • Lee, Hyo-Ku (Department of Food Science, Kongju National University) ;
  • Rhee, Seong-Kap (Department of Food and Biotechnology, Hoseo University) ;
  • Hong, Hee-Do (Korea Food Research Institute) ;
  • Choi, Hyeon-Son (Department of Food Science and Biotechnology, CHA University) ;
  • Lee, Boo-Yong (Department of Food Science and Biotechnology, CHA University) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • 윤보라 (강원대학교 식품생명공학과) ;
  • 이영준 (강원대학교 식품생명공학과) ;
  • 김선구 (공주대학교 식품공학과) ;
  • 장중영 (공주대학교 식품공학과) ;
  • 이효구 (공주대학교 식품공학과) ;
  • 이성갑 (호서대학교 식품생물공학과) ;
  • 홍희도 (한국식품연구원) ;
  • 최현선 (차의과학대학교 식품생명공학과) ;
  • 이부용 (차의과학대학교 식품생명공학과) ;
  • 이옥환 (강원대학교 식품생명공학과)
  • Received : 2011.12.29
  • Accepted : 2012.05.18
  • Published : 2012.06.30
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Abstract

Recently, NADPH oxidase 4 (NOX4)-mediated generation of intracellular reactive oxygen species (ROS) was proposed to accelerate adipogenesis of 3T3-L1 cell. We have previously shown that Cheonnyuncho (Opuntia humifusa) extract significantly inhibited adipocyte differentiation via downregulation of $PPAR{\gamma}$ (peroxisome proliferator-activated receptor gamma) gene expression. In this study, we focused on the molecular mechanism(s) of NOX4, G6PDH (glucose-6-phosphate dehydrogenase) and antioxidant enzymes in anti-oxidative activities of 3T3-L1 adipocytes. Our results indicate that Cheonnyuncho extracts markedly inhibits ROS production during adipogenesis in 3T3-L1 cells. Cheonnyuncho extracts suppressed the mRNA expression of the pro-oxidant enzyme such as NOX4 and the NADPH-producing G6PDH enzyme. In addition, treatment with Cheonnyuncho extract was found to upregulate mRNA levels of antioxidant enzymes such as Mn-SOD (manganese-superoxide dismutase), Cu/Zn-SOD (copper/zinc-SOD), glutathione peroxidase (GPx), glutathion reductase (GR), and catalase, all of which are important for endogenous antioxidant responses. These data suggest that Cheonnyuncho extract may be effective in preventing the rise of oxidative stress during adipocyte differentiation through mechanism(s) that involves direct down regulation of NOX4 and G6PDH gene expression or via upregulation of endogenous antioxidant responses.

최근 연구에 의하면, NOX4에 의해 생성된 세포내 ROS는 지방세포의 분화를 촉진하는 것으로 알려져 있다. 본 연구진의 보고에 의하면 천년초 추출물은 $PPAR{\gamma}$의 발현 및 지방세포의 분화를 억제하는 것으로 밝혀진 바 있다. 따라서 본 연구에서는 천년초 선인장의 건강기능식품 소재화시 기초자료를 제공하고자 지방세포내 생성되는 ROS에 대한 천년초 열수 및 에탄올 추출물의 억제효과 및 주요 유전자(NOX4, G6PDH 및 항산화효소)의 발현 양상을 조사하였다. 천년초 열수 및 에탄올 추출물을 처리한 지방세포에서는 대조군에 비해 ROS 생성량이 유의적으로 감소하는 것으로 나타내었고, 추출용매별 천년초 추출물의 ROS 저감효능은 열수 추출물에 비해 에탄올 추출물에서 높게 나타났다. 천년초 추출물을 처리한 지방세포에서는 ROS 생성과 연관된 주요 유전자 NOX4 및 G6PDH의 발현이 대조군에 비해 감소하는 경향을 나타낸 반면, Cu/Zn-SOD, Mn-SOD, catalase, GPx 등과 같은 항산화효소의 발현은 대조군에 비해 증가한 것으로 나타났다. 이상의 결과들로 볼 때, 천년초 추출물은 지방세포의 분화과정에서 ROS의 생성을 억제하고 항산화효소의 발현을 증가시켜 ROS에 의한 산화적 스트레스를 효과적으로 저감화하는 것으로 밝혀졌다.

Keywords

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