Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Effect of Tissue Factor on Invasion Inhibition and Apoptosis Inducing Effect of Oxaliplatin in Human Gastric Cancer Cell

  • Yu, Yong-Jiang (Department of General Surgery, First Hospital of Lanzhou Univercity) ;
  • Li, Yu-Min (Department of General Surgery, Second Hospital of Lanzhou Univercity) ;
  • Hou, Xu-Dong (Department of General Surgery, First Hospital of Lanzhou Univercity) ;
  • Guo, Chao (Department of General Surgery, First Hospital of Lanzhou Univercity) ;
  • Cao, Nong (Department of General Surgery, First Hospital of Lanzhou Univercity) ;
  • Jiao, Zuo-Yi (Department of General Surgery, Second Hospital of Lanzhou Univercity)
  • Published : 2012.05.30
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Abstract

Objective: Tissue factor (TF) is expressed abnormally in certain types of tumor cells, closely related to invasion and metastasis. The aim of this study was to construct a human gastric cancer cell line SGC7901 stably-transfected with human TF, and observe effects on oxaliplatin-dependent inhibition of invasion and the apoptosis induction. Methods: The target gene TF was obtained from human placenta by nested PCR and introduced into the human gastric cell line SGC7901 through transfection mediated by lipofectamine. Stably-transfected cells were screened using G418. Examples successfully transfected with TF-pcDNA3 recombinant (experimental group), and empty vector pcDNA3 (control group) were incubated with oxaliplatin. Transwell chambers were used to show change in invasive ability. Caspase-3 activity was detected using a colorimetric method and annexin-V/PI double-staining was applied to detect apoptosis. Results: We generated the human gastric cancer cell line SGC7901/TF successfully, expressing TF stably and efficiently. Compared with the control group, invasion increased, whereas caspase-3 activity and apoptosis rate were decreased in the experimental group. Conclusion: TF can enhance the invasive capacity of gastric cancer cells in vitro. Its increased expression may reduce invasion inhibition and apoptosis-inducing effects of oxaliplatin and therefore may warrant targeting for improved chemotherapy.

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References

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