Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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DNA Sequencing Analysis Technique by Using Solid-State Nanopore

고체상 나노구멍을 이용한 DNA 염기서열 분석기술

  • 김태헌 (고려대학교 전기전자전파공학부) ;
  • 박정호 (고려대학교 전기전자전파공학부)
  • Received : 2012.07.17
  • Accepted : 2012.09.12
  • Published : 2012.09.30
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Abstract

Nanopore DNA sequencing is an emerging and promising technique that can potentially realize the goal of a low-cost and high-throughput method for analyzing human genome. Especially, solid-state nanopores have relatively high mechanical stability, simple surface modification, and facile fabrication process without the need for labeling or amplification of PCR (polymerized chain reaction) in DNA sequencing. For these advantages of solid-sate nanopores, the use of solid-state nanopores has been extensively considered for developing a next generation DNA sequencing technology. Solid-state nanopore sequencing technique can determine and count charged molecules such as single-stranded DNA, double-stranded DNA, or RNA when they are driven to pass through a membrane nanopore between two electrolytes of cis-trans chambers with applied bias voltage by measuring the ionic current which varies due to the existence of the charged particles in the nanopore. Recently, many researchers have suggested that nanopore-based sensors can be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000.

Keywords

Acknowledgement

Supported by : 한국연구재단

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