Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Clinical features of fish with pathogens isolated from emaciated olive flounder Paralichthys olivaceus

여윔증상 넙치, Paralichthys olivaceus로부터 분리된 병원균의 임상적 고찰

  • Choi, Hye-Sung (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Jun, Lyu-Jin (School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University) ;
  • Kim, Seoung-Min (School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University) ;
  • Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Yi-Kyung (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Lim, Hee-Young (Institute for Clinical Pharmacology, Medical Center of Goethe University) ;
  • Yeo, In-Kyu (School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University) ;
  • Jeong, Joon-Bum (School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University)
  • 최혜승 (국립수산과학원 병리연구과) ;
  • 전려진 (제주대학교 해양의생명과학부) ;
  • 김승민 (제주대학교 해양의생명과학부) ;
  • 정현도 (부경대학교 수산생명의학과) ;
  • 김이경 (부경대학교 수산생명의학과) ;
  • 임희영 (괴태대학 의학센터) ;
  • 여인규 (제주대학교 해양의생명과학부) ;
  • 정준범 (제주대학교 해양의생명과학부)
  • Received : 2012.07.31
  • Accepted : 2012.08.13
  • Published : 2012.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Two bacteria strains were isolated from emaciated olive flounder (Paralichthys olivaceus) in aquafarm and were identificated as Vibrio harveyi (JV1) and Edwardsiella tarda (JE1), respectively. In the challenge experiments, we found 100% cumulative mortalities in all of olive flounder injected with JV1, JE1 or JV1+JE1 within eleven days after the injection. Two bacteria strains were reisolated from dead fish and were analyzed using the PCR method. In the physilogical analysis, the hematocrit, AST, ALT and cholesterol levels in experimental groups were increased significantly compared to those in control group, but the glucose, total protein and triglyceride levels were significantly decreased. Additionally, the lysozyme activity in the blood serum was decreased. The histopathological observations of the intestine showed that all groups had detachment and destruction of epithelial tissues except for the control group.

Keywords

References

  1. Alvarez, J.J., Austin, B., Alvarze, A.M. and Reyes, H.: Vibrio harveyi: A pathogen of penaeid shirimps and fish in Venezuela. J. Fish Dis., 21: 313-316, 1998. https://doi.org/10.1046/j.1365-2761.1998.00101.x
  2. Kanai, K.: Bacterial diseases of flounder, Paralichthys olivaceus. 한국어병학회지, 6: 197-208, 1993.
  3. Castro, N., Toranzo, A.E., Núñez, S., Osorio, C.R. and Magariños, B.: Evaluation of four polymerase chain reaction primer pairs for the detection of Edwardsiella tarda in turbot. Dis. Aquat. Org., 90: 55-61, 2010. https://doi.org/10.3354/dao02203
  4. Mohandas, A., Cheng, T.C. and Cheng, J.B.: Mechanism of lysosomal enzyme release from Mercenaria mercenaria granulocyte: a scanning electron microscope study. J. Invertebr. Pathol., 46: 189-197, 1985. https://doi.org/10.1016/0022-2011(85)90148-X
  5. Nakatsugawa, T.: Edwardsiella tarda isolated from cultured young flounder. Fish Pathol., 18: 99-101, 1983. https://doi.org/10.3147/jsfp.18.99
  6. Ogawa, K. and Yokoyama, H.: Emaciation disease of cultured tiger puffer Takifugu rubripes. Bull. Natl. Res. Inst. Aquacult., Suppl., 5: 65-70, 2001.
  7. Parry, R.M., Chandau, R.C. and Shahani, R.M.: A rapid and sensitive assay of muramidase. Proc. Soc. Exp. Biol. Med., 119: 384-386, 1965. https://doi.org/10.3181/00379727-119-30188
  8. Soffientino, B., Gwaltney, T., Nelson, D.R., Speck, J.L., Mauel, M. and Go'mez-Chiarri, M.: Infectious necrotizing enteritis and mortality caused by Vibrio carchariae in summer flounder Paralichthys dentatus during intensive culture. Dis. Aquat. Org., 38: 201-210, 1999. https://doi.org/10.3354/dao038201
  9. Takahashi, Y., Itami, T. and Konegawa, K.: Enzymatic properties of partially lysozyme from the skin mucus of carp. Bull. Jap. Soc. Sci. Fish. 52: 1209-1214, 1986. https://doi.org/10.2331/suisan.52.1209
  10. Tin, T., Yokoyama, H., Ogawa, K. and Wakabayashi, H.: Myxosporeans and their hyperparasitic microsporeans in the intestine of emaciated tiger puffer. Fish Pathol., 35: 145-156, 2000. https://doi.org/10.3147/jsfp.35.145
  11. Tin, T., Ogawa, K. and Wakabayashi, H.: Pathological changes induced by three myxosporeans in the intestine of cultured tiger puffer, Takifugu rubripes (Temminck and Schlegel). J. Fish Dis., 25: 63-72, 2002. https://doi.org/10.1046/j.1365-2761.2002.00333.x
  12. Yasuda, H., Ooyama, T., Nakamura, A., Iwata, K., Palenzuela, O. and Yokoyama, H.: Occurrence of the Myxosporean emaciation disease caused by Enteromyxum leei in cultured Japanese flounder Paralichthys olivaceus. Fish Pathol., 40: 175-180, 2005. https://doi.org/10.3147/jsfp.40.175
  13. 강철영, 강봉조, 문영건, 김기영, 허문수: 제주도 양식넙치(Paralichthys olivaceus)로부터 분리한 비 용혈성 연쇄구균의 동정. 한국어병학회지, 20: 109-117, 2007.
  14. 김진우, 정승희, 박명애, 도정완, 최동림, 지보영, 조미영, 김명석, 최혜승, 김이청, 이주석, 이창훈, 방종득, 박미선, 서정수: 2000년-2006년 하절기 양식어류의 병원체 감염현황. 한국어병학회지, 19: 207-214, 2006.
  15. 오윤경: 해산 양식어류에서 분리된 Vibrio harveyi의 신속 동정과 항원성에 대한 연구. 부경대학교 석사학위논문 1-53, 2008.
  16. 원경미, 최정현, 박수일: 배양 조건에 따른 Vibrio harveyi extracellular products (ECP)의 특성. 한국어병학회지, 19: 119-126, 2006.

Cited by

  1. Characterization of the Myxosporean Parasite Isolated from Emaciated Olive Flounders Paralichthys olivaceus on Jeju Island vol.48, pp.3, 2015, https://doi.org/10.5657/KFAS.2015.0337
  2. The Infection Characteristics of Vibrio scophthalmi Isolated from Olive Flounder, Paralichthys olivaceus vol.26, pp.3, 2013, https://doi.org/10.7847/jfp.2013.26.3.207
  3. Enteromyxum leei (Myxosporea: Bivalvulida) as the cause of myxosporean emaciation disease of farmed olive flounders (Paralichthys olivaceus) and a turbot (Scophthalmus maximus) on Jeju Island, Korea vol.115, pp.11, 2016, https://doi.org/10.1007/s00436-016-5200-5
  4. Application of water-soluble tetrazolium salt for development of rapid antimicrobial susceptibility testing methods vol.28, pp.2, 2015, https://doi.org/10.7847/jfp.2015.28.2.071
  5. Hematological Analysis and Non-specific Immune Responses of Emaciated Olive Flounder, Paralichthys olivaceus in Korea vol.29, pp.6, 2017, https://doi.org/10.13000/JFMSE.2017.29.6.1758
  6. (Temminck and Schlegel) pp.01407775, 2019, https://doi.org/10.1111/jfd.12912
  7. 톨트라주릴(Toltrazuril)을 이용한 제주도 넙치(Paralichthys olivaceus)의 점액포자충성 여윔증에 대한 치료법 연구 vol.33, pp.1, 2012, https://doi.org/10.7847/jfp.2020.33.1.055