Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Temperature Condition on Nitrogen Mineralization and Soil Microbial Community Shift in Volcanic Ash Soil

온도가 화산회토양의 질소무기화와 미생물군집이동에 미치는 영향

  • Joa, Jae-Ho (National Institute of Horticultural & Herbal Science, RDA) ;
  • Moon, Doo-Gyung (National Institute of Horticultural & Herbal Science, RDA) ;
  • Koh, Sang-Wook (National Institute of Horticultural & Herbal Science, RDA) ;
  • Hyun, Hae-Nam (Major of Plant Resources and Environment, Jeju National University)
  • Received : 2012.03.19
  • Accepted : 2012.07.10
  • Published : 2012.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to evaluate effect of temperature condition on nitrogen mineralization of organic matter, distribution of microbial group by PLFA profiles, and soil microbial community structure in volcanic ash soil. Dried soil 30 g mixed well each 2 g of pellet (OFPE) organic fertilizers, pig manure compost (PMC), and food waste compost (FWC). And then had incubated at $10^{\circ}C$, $20^{\circ}C$, and $30^{\circ}C$, respectively. Nitrogen mineralization rate increased with increasing temperature and that was in the order of FWC>OFPE>PMC. Distribution ratio of microbial group by PLFA profiles were different significantly caused by incubation temperature and the type of organic matter. As incubating time passed, density of microbial group decreased gradually. The Gram-bacteria PLFA/Gram+ bacteria PLFA, Fungi PLFA/Bacteria PLFA, and Unsaturated PLFA/saturated PLFA ratios were decreased according to the increasing temperature gradually. But cy19:0/$18:1{\omega}7c$ ratio increased both FWC and PMC treatment. Principal component analysis using PLFA profiles showed that microbial community structure made up clearly at both 75 days ($10^{\circ}C$) and 270 days ($30^{\circ}C$) by temperature factor. As incubating time passed, microbial community structure shifted gradually.

본 연구는 토양과 온도조건이 질소무기화율, 인지질 지방산유래 미생물 분포와 군집구조에 미치는 영향을 평가하고자 수행하였다. 화산회토양 30 g에 입상유기질비료, 음식물퇴비, 돈분퇴비를 각각 2 g씩 잘 혼합한 후 $10^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$에서 항온배양을 하면서 질소 무기화율과 인지질 지방산 함량을 분석하였다. 질소 무기화율은 온도와 비례하여 증가하였으며 음식물퇴비>입상유기질비료>돈분퇴비 순으로 질소무기화율이 높았다. 지방산 유래 미생물 그룹의 분포는 온도, 유기물종류에 따라 차이를 보였으며 시간이 경과 할수록 미생물의 밀도는 감소하는 경향을 나타냈다. G-/G+, F/B, Unsat/sat 비는 온도가 $10^{\circ}C$씩 올라갈수록 감소하였고 cy19:0/$18:1{\omega}7c$비는 음식물퇴비와 돈분퇴비에서 증가하였다. PLFA 함량을 이용한 주성분 분석 결과 초기 (75일)는 $10^{\circ}C$, 후기 (270일)는 $30^{\circ}C$에서 온도요인에 따라 뚜렷하게 미생물 군집을 보였으며 시간이 경과 할수록 군집이동이 나타냈다.

Keywords

References

  1. Agehara, S. and D.D. Warncke. 2004. Soil moisture and temperature effects on nitrogen release from organic nitrogen sources. Am. J. Soil Sci. Soc. 69:1844-1855.
  2. Bapiri, A., E. Bååth, and J. Rousk. 2010. Drying-rewetting cycles affect fungal and bacterial growth differently in an arable soil. Microb. Ecol. 60:419-428. https://doi.org/10.1007/s00248-010-9723-5
  3. Bardgett, R.D., P.J. Hobbs, and A. Frostegard. 1996. Changes in soil fungal: bacterial biomass ratios following reductions in the intensity of management of an upland grassland. Biol. Fertil. Soils 22:261-264. https://doi.org/10.1007/BF00382522
  4. Bligh, E.G. and W.J. Dyer. 1959. A rapid method for total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917. https://doi.org/10.1139/o59-099
  5. Bossio, D.A., K.M. Scow, N. Gunapala, and K.J. Graham. 1998. Determinants of soil microbial communities: effects of agricultural management, season, and soil type on phospholipid fatty acid profiles. Microb. Ecol. 36:1-12. https://doi.org/10.1007/s002489900087
  6. Bossio D.A. and K.M. Scow. 1998. Impacts of carbon and flooding on soil microbial communities: phopholipid fatty acid profiles and substrate utilization patterns. Microb. Ecol. 35:265-278. https://doi.org/10.1007/s002489900082
  7. de Ridder-Duine, A.S., G.A. Kowalchuk, P.J.A.K. Gunnewiek, W. Smant, J.A. van Veen, and W. de Boer. 2005. Rhizosphere bacterial community composition in natural stands of Carex arenaria (sand sedge) is determined by bulk soil community composition. Soil Biol. Biochem. 37:349-357. https://doi.org/10.1016/j.soilbio.2004.08.005
  8. Deenik, J. 2006. Nitrogen mineralization potential in important agricultural soils of Hawai'i. Soil Crop Manage. 15:1-5.
  9. Feng, X. and M.J. Simpson. 2009. Temperature and substrate controls on microbial phospholipid fatty acid composition during incubation of grassland soils contrasting in organic matter quality. Soil Biol. Biochem. 41:804-812. https://doi.org/10.1016/j.soilbio.2009.01.020
  10. Joa, J.H., D.G. Moon, S.J. Chun, C.H. Kim, K.S. Choi, H.N. Hyun, and U.G. Kang.. 2009. Effect of temperature on soil microbial biomass, enzyme activities, and PLFA content during incubation period of soil treated with organic materials. Korean J. Soil Sci. Fert. 42:500-512.
  11. Joa, J.H., K.H. Moon, S.C. Kim, D.G. Moon, and S.W. Koh. 2012. Effect of temperature condition on nitrogen mineralization of organic matter and soil microbial community structure in non-volcanic ash soil. Korean J. Soil Sci. Fert. 45:377-384. https://doi.org/10.7745/KJSSF.2012.45.3.377
  12. Kaur, A., A. Chaudhary, R. Choudhary, and R. Kaushik. 2005. Phospholipid fatty acid-A bioindicator of environment monitoring and assessment in soil ecosystem. Current Science 89:1103-1112.
  13. Kemmitt, S.J., D. Wright, K.W.T. Goulding, and D.L. Jones. 2006. pH regulation of carbon and nitrogen dynamics in two agricultural soils. Soil Biol. Biochem. 38:898-911. https://doi.org/10.1016/j.soilbio.2005.08.006
  14. Li, W.H., C.B. Zhang, H.B. Jiang, G.R. Xin, and Z.Y. Yang. 2006. Changes in soil microbial community associated with invasion of the exotic weed Mikania micrantha H. B. K. Plant Soil 281:309-324. https://doi.org/10.1007/s11104-005-9641-3
  15. Manzoni, S. and A. Porpotato. 2007. A theoretical analysis of nonlinearities and feedbacks in soil carbon and nitrogen cycles. Soil Biol. Biochem. 39:1542-1556. https://doi.org/10.1016/j.soilbio.2007.01.006
  16. Marschner, P., E. Kandeler, and B. Marschner. 2003. Structure and function of the soil microbial community in a long-term fertilizer experiment. Soil Biol. Biochem. 35:453- 461. https://doi.org/10.1016/S0038-0717(02)00297-3
  17. Mondini, C., M.L. Cayuela, T. Sinicco, M.A.S. Monedero, E. Bertolone, and L. Bardi. 2008. Soil application of meat and bone meal. Short-term effects on mineralization dynamics an soil biochemical and microbiological properties. Soil Biol. Biochem. 40:462-474. https://doi.org/10.1016/j.soilbio.2007.09.010
  18. Nobili, D.M., M. Contin, and P.C. Brookes. 2006. Microbial biomass dynamics in recently air-dried and rewetted soils compared to others stored air-dry for up to 103 years. Soil Biol. Biochem. 38:2871-2881. https://doi.org/10.1016/j.soilbio.2006.04.044
  19. NIAST. 2000. Methods of soil and plant analysis. National Institute of Agricultural Science and Technology, RDA, Suwon, Korea.
  20. Nunan, N., T.J. Daniell, B.K. Singh, A. Papert, J.W. McNicol, and J.I. Prosser. 2005. Links between plant and rhizoplane bacterial communities in grassland soils characterized using molecular techniques. Appl. Environ. Microbiol. 71:6784-6792. https://doi.org/10.1128/AEM.71.11.6784-6792.2005
  21. Rahman, M.H. and S. Sugiyama. 2008. Dynamics of microbial community in Japanese andisol of apple orchard production systems. Commun. Soil Sci. Plan. 39:1630-1657. https://doi.org/10.1080/00103620802071853
  22. Reichstein, M., T. Katter, O. Andren, P. Ciais, E.D. Schulze, W. Cramer, D. Papale, and R. Valentini. 2005. Temperature sensitivity of decomposition in relation to soil organic matter pools: critique and outlook. Biogeosciences 2:317-321. https://doi.org/10.5194/bg-2-317-2005
  23. Rosa, M., J.A. Pascuala, C. Garciaa, M.T. Hernandeza, and H. Insam. 2006. Hydrolase activities, microbial biomass and bacterial community in a soil after long-term amendment with different composts. Soil Biolo. Biochem. 38:3443-3452. https://doi.org/10.1016/j.soilbio.2006.05.017
  24. Takenaka, M and K. Hayano. 1999. Investigation on the influence of the global warming on the mineralization of soil organic matter. Research Outcomes, Agriculture, Forestry Fishery Technology Bureau. 339:232-236.
  25. Tscherko, D., and E. Kandeler. 1999. Classification and monitoring of soil microbial biomass, N-mineralization and enzyme activities to indicate environmental changes. Die Bodenkultur. 50:215-226
  26. Wang, W., C.J. Smith, P.M. Chalk, and D. Chen. 2001. Evaluation chemical and physical indices of nitrogen mineralization capacity with an unequivocal reference. Soil Sci. Soc. Am. J. 65:368-376. https://doi.org/10.2136/sssaj2001.652368x
  27. Webster, G., L.C. Watt, J. Rinna, J.C. Fry, R.P. Evershed, R.J. Parkes, and A.J. Weightman. 2006. A comparison of stableisotope probing of DNA and phospholipid fatty acids to study prokaryotic functional diversity in sulfate-reducing marine sediment enrichment slurries. Environ. Microb.8: 1575-1589. https://doi.org/10.1111/j.1462-2920.2006.01048.x
  28. Wu, Y., X. Yu, H. Wang, N. Ding, and J. Xu. 2010. Does history matter? Temperature effects on soil microbial biomass and community structure based on the phospholipid fatty acid (PLFA) analysis. J. Soils Sediments 10:223-230. https://doi.org/10.1007/s11368-009-0118-5
  29. Yao, H., Z. He, M.J. Wilson, and C.D. Campbell. 2000. Microbial biomass and community structure in a sequence of soils with increasing fertility and changing land use. Microb. Ecol. 40:223-237.

Cited by

  1. Impacts of Cropping Systems on the Distribution of Soil Microorganisms in Mid-mountainous Paddy vol.49, pp.5, 2016, https://doi.org/10.7745/KJSSF.2016.49.5.480
  2. An Investigation of the Hazards Associated with Cucumber and Hot Pepper Cultivation Areas to Establish a Good Agricultural Practices (GAP) Model vol.46, pp.1, 2014, https://doi.org/10.9721/KJFST.2014.46.1.108
  3. Effect of Seed Density, Number of Seeds Sown Per Hole and Thinning Treatment on Growth Characteristics and Disease Occurrence in Greenhouse-Cultivated Ginseng vol.23, pp.3, 2015, https://doi.org/10.7783/KJMCS.2015.23.3.198