Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Monitoring of Pathogenic Bacteria in Organic Vegetables from Korean Market

국내 유통중인 유기농 채소류의 미생물 분포도 분석

  • 정규석 (농촌진흥청 국립농업과학원) ;
  • 노은정 (농촌진흥청 국립농업과학원) ;
  • 류경열 (농촌진흥청 국립농업과학원) ;
  • 김원일 (농촌진흥청 국립농업과학원) ;
  • 박경훈 (농촌진흥청 국립농업과학원) ;
  • 이동환 (농촌진흥청 국립농업과학원) ;
  • 김계훈 (서울시립대학교 환경원예학과) ;
  • 윤종철 (농촌진흥청 국립농업과학원) ;
  • 허성기 (농촌진흥청 국립농업과학원)
  • Received : 2012.07.06
  • Accepted : 2012.08.14
  • Published : 2012.08.31
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Abstract

This study was undertaken to assess the microbiological quality and prevalence of pathogens in organic vegetables produced in Korea. A total of 189 organically grown vegetable samples (perilla leaf 50, lettuce 50, tomato 39, cucumber 50) were analyzed for the presence of aerobic plate count, Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, and Yersinia enterocolitica. The total aerobic plate counts were in the range of 4.2 to $7.7log\;CFU\;g^{-1}$ for perilla leaf, 5.0 to $8.0log\;CFU\;g^{-1}$ for lettuce, 4.0 to $7.5log\;CFU\;g^{-1}$ for tomato, and 6.6 to $8.6log\;CFU\;g^{-1}$ for cucumber. The highest counts were found in cucumber. E. coli O157:H7, Salmonella spp., S. aureus, L. monocytogenes, and Y. enterocolitica were not detected from any organically grown vegetable samples. This research suggests that continuous monitoring in organic vegetables is required to improve fresh produce safety.

최근에 건강식품의 선호에 따라 신선 채소류 등의 소비가 증가하면서 농산물의 안전성에 대한 관심이 점점 증가하고 있다. 이에 본 연구는 국내 유통 중인 유기농 채소류를 구입하여 총균수, Salmonella spp., E. coli O157:H7, S. aureus, L. monocytogenes, Y. enterocolitica 등의 병원성 미생물의 오염도를 분석하고 유기농산물의 식중독균에 대한 잠재적인 위험성을 평가하는 기초자료에 도움을 주고자 수행하였다. 유기농 채소류 4종의 일반 세균수 수준을 비교한 결과 오이의 일반 세균수 수준이 가장 높았고, 토마토의 일반 세균수는 가장 낮았다. 유기농 채소 중 깻잎의 일반 세균수는 $4.2{\sim}7.7log\;CFU\;g^{-1}$, 상추는 $5.0{\sim}8.0log\;CFU\;g^{-1}$, 토마토는 $4.0{\sim}7.5log\;CFU\;g^{-1}$, 오이는 $6.6{\sim}8.6log\;CFU\;g^{-1}$ 범위였다. 유기농 채소에서 Salmonella spp., E. coli O157:H7, S. aureus, L. monocytogenes, Y. enterocolitica 등은 검출되지 않았다. 일반 세균수는 관행 농산물과 비슷한 수준을 보였으며 병원성 미생물은 전혀 검출되지 않았기 때문에 식중독균 오염수준은 극히 낮을 것이라고 생각한다. 그런데 오염될 수 있는 가능성이 있으므로 생산, 유통 단계에서 주의 깊은 관리가 필요하다고 판단된다.

Keywords

References

  1. Ahn, Y.S. and D.H. Shin. 1999. Antimicrobial effects of organic acids and ethanol on several foodborne microorganism. Korean J. Food Sci. Technol. 31:1315-1323.
  2. Bae, Y.M., Y.J. Hong, D.H. Kang, S.G. Heu, and S.Y. Lee. 2011. Microbial and pathogenic contamination of ready-to-eat fresh vegetables in Korea. Korean J. Food Sci. Technol. 43:161-168. https://doi.org/10.9721/KJFST.2011.43.2.161
  3. Beuchat, L.R. 1996. Pathogenic microorganisms associated with fresh produce. J. Food Prot. 59:204-216.
  4. Bohaychuk, V.M., R.W. Bradbury, R. Dimock, M. Fehr, G.E. Gensler, R.K. King, R. Rieve, and P.R. Barrios. 2009. A microbiological survey of selected Alberta-grown fresh produce from farmers' markets in Alberta, Canada. J. Food Prot. 72:415-420.
  5. Burnett, S.L. and L.R. Beuchat. 2001. Human pathogens associated with raw produce and unpasteurized juices, and difficulties in decontamination. J. Industrial Micro. & Biotech. 27:104-110. https://doi.org/10.1038/sj.jim.7000199
  6. Cho, J.I., G.J. Bahk, K.S. Kim, and S.D. Ha. 2004. Microbial assessment of wild cabbage and its control. Korean J. Food Sci. Technol. 36:162-167.
  7. Cho, M.J., A.R. Jeong, H.J. Kim, N.R. Lee, S.W. Oh, Y.J. Kim, H.S. Chun, and M.S. Koo. 2011. Microbiological quality of fresh-cut produce and organic vegetables. Kor. J. Food Sci. Technol. 43:91-97. https://doi.org/10.9721/KJFST.2011.43.1.091
  8. Donelly, C.W. and E.H. Briggs. 1986. Psychrotropic growth and themal inactivation of Listeria monocytogenes as a function of milk composition. J. Food Prot. 49:994-998.
  9. Jung, S.H., M.J. Hur, J.H. Ju, K.A. Kim, S.S. Oh, J.M. Go, Y.H. Kim, and J.S. Im. 2006. Microbiological evaluation of raw vegetables. Korean J. Fd. Hyg. Safety 21:250-257.
  10. Kang, T.M., S.K. Cho, J.Y. Park, K.B. Song, M.S. Chung, and J.H. Park. 2011. Analysis of microbial contamination of sprouts and fresh cut salads in a market. Korean J. Food Sci. Technol. 43:490-494. https://doi.org/10.9721/KJFST.2011.43.4.490
  11. Kim, D.M. and S.I. Hong. 2004. Current status and prospect of fresh-cut produce in Korea. Food Preserv. Proc. Ind. 4:41-61.
  12. Lammerding, A.M. 1997. An overview of microbial food safety risk assessment. J. Food Prot. 60:1420-1425.
  13. Lee, Y.S., S.H. Kim, and D.H. Kim. 2009. Current status of fresh-cut market in Korea and stimulus measures. Korea Rural Economic Institute Report. 602:16-19.
  14. Loncarevic, S., G.S. Johannessen, and L.M. Rorvik. 2005. Bacteriological quality of organically grown leaf lettuce in Norway. Lett. Appl. Microbiol. 41:186-189. https://doi.org/10.1111/j.1472-765X.2005.01730.x
  15. Mukherjee, A., D. Speh, E. Dyck, and F. Diez-Gonzalez. 2004. Preharvest evaluation of coliforms, Escherichia coli, Salmonella, and Escherichia coli O157:H7 in organic and conventional produce grown by Minnesota farmers. J. Food Prot. 67:894-900.
  16. Nguz, K., J. Shindano, S. Samapundo, and A. Huyghebaert. 2005. Microbiogical evaluation of fresh-cut vegetables produced in Zambia. Food Control. 16:623-628. https://doi.org/10.1016/j.foodcont.2004.07.001
  17. Oliveira, M., J. Usall, I. Vinas, M. Anguera, F. Gatius, and M. Abadias. 2010. Microbiological quality of fresh lettuce from organic and conventional production. Int. J. Food Microbiol. 27:679-684. https://doi.org/10.1016/j.fm.2010.03.008
  18. Sagoo, S.K., C.L. Little, and R.T. Mitchell. 2001. The microbiological examination of ready-to-eat organic vegetables from retail establishments in the United Kingdom. Lett. Appl. Microbiol. 33:434-439. https://doi.org/10.1046/j.1472-765X.2001.01026.x
  19. Smith, D.W., C.K. Barlow, L. Alderton, and M.F. Jacobson. 2011. Outbreak alert! Center for Science in the Public Interest. Available at: http://www.cspint.org/reports/outbreak_report.pdf Accessed Aug. 15.
  20. Stephenson, J. 1997. New approaches for detecting and curtailing foodborne microbial infections. J. American Med. Association. 277:1337-1339. https://doi.org/10.1001/jama.1997.03540410015004
  21. Wher, M.H. 1987. Listeria monocytogenes-A current dilemma. J. Assoc. Off. Anal. Chem. 70:769.

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