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Study on Development of Non-Destructive Measurement Technique for Viability of Lettuce Seed (Lactuca sativa L) Using Hyperspectral Reflectance Imaging

초분광 반사광 영상을 이용한 상추(Lactuca sativa L) 종자의 활력 비파괴측정기술 개발에 관한 연구

  • Ahn, Chi-Kook (College of Agriculture and Life Science, Chungnam National University) ;
  • Cho, Byoung-Kwan (College of Agriculture and Life Science, Chungnam National University) ;
  • Mo, Chang Yeun ;
  • Kim, Moon S. (Environmental Microbial and Food Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture)
  • 안치국 (충남대학교 바이오시스템 기계공학과) ;
  • 조병관 (충남대학교 바이오시스템 기계공학과) ;
  • 모창연 (농촌진흥청 국립농업과학원 농업공학부) ;
  • Received : 2012.08.01
  • Accepted : 2012.09.26
  • Published : 2012.10.30

Abstract

In this study, the feasibility of hyperspectral reflectance imaging technique was investigated for the discrimination of viable and non-viable lettuce seeds. The spectral data of hyperspectral reflectance images with the spectral range between 750 nm and 1000 nm were used to develop PLS-DA model for the classification of viable and non-viable lettuce seeds. The discrimination accuracy of the calibration set was 81.6% and that of the test set was 81.2%. The image analysis method was developed to construct the discriminant images of non-viable seeds with the developed PLS-DA model. The discrimination accuracy obtained from the resultant image were 91%, which showed the feasibility of hyperspectral reflectance imaging technique for the mass discrimination of non-viable lettuce seeds from viable ones.

본 연구에서는 초분광 반사광 영상기술을 이용하여 비파괴적으로 상추의 건전종자와 퇴화종자를 선별하는 기술을 개발하고자 하였다. 750~1000nm의 근적외선 초분광 반사광 영상의 분광데이터를 이용하여 상추의 발아종자와 불발아 종자를 판별하는 PLS-DA 모델을 개발하고 개발된 모델의 성능 평가를 실시하였다. 모델 calibration의 판별 정확도는 81.6%였으며, test의 결과는 81.2%의 판별 정확도를 보였다. 또한 개발된 PLS-DA 모델을 적용한 초분광 반사광 영상을 이용하여 대량의 불발아 종자를 동시에 영상으로 검출 가능한 영상처리 알고리즘을 개발하였다. 초분광 반사광 영상에 PLS-DA 모델이 적용된 영상을 이용한 검출 정확도는 91%로 나타났으며, 이는 초분광 반사광 영상을 이용하여 대량의 상추 종자의 비파괴 품질선별에 이용될 수 있음을 보여 주었다.

Keywords

References

  1. F. B. Abeles, "Role of ethylene in Lactuca sativa cv 'Grand Rapids' seed germination," Plant Physiology, Vol. 81, No. 1, pp. 780-787 (1986) https://doi.org/10.1104/pp.81.3.780
  2. T. Gonai, S. Kawahara, M Tougou, S. Satoh, T. Hashiba, N. Hiral, H. Kawaide, Y. Kamiya and T. Yoshioka, "Abscisic acid in the thermoinhibition of lettuce seed germination and enhancement of its catabolism by gibberellin," Journal of Experimental Botany, Vol. 55, pp. 111-118 (2004)
  3. J. R. M. Dunlap and P. W. Morgan, "Reversal of induced dormancy in lettuce by ethylene, kinetin, and gibberellic acid," Plant Physiology, Vol. 60, No. 2, pp. 222-224 (1997)
  4. H. J. Hwang, J. M. Lee, S. Y. Kim and G. W. Choi, "Seed germination in lettuce affected by light quality and plant growth regulators," Journal of Bio-Environment Control, Vol. 17, No. 1, pp. 51-59 (2008)
  5. N. Shetty, T. G. Min, M. H. Olesen and B. Boelt, "Optimal sample size for predicting viability of cabbage and radish seeds based on near infrared spectra of single seeds," Journal of Near Infrared Spectroscopy, Vol. 19, pp. 451-461 (2011) https://doi.org/10.1255/jnirs.966
  6. C. K. Ahn, B. K. Cho, J. S. Kang and K. J. Lee, "Study on non-destructive sorting technique for lettuce(Lactuca sativa L) seed using Fourier transform near-infrared spectrometer," CNU Journal of Agricultural Science, Vol. 39, No. 1, pp. 111-116 (2012) https://doi.org/10.7744/cnujas.2012.39.1.111
  7. Y. L. Liu, Y. R. Chen, M. S. Kim, D. E. Chen and A. M. Lefcourt, "Development of simple algorithms for the detection of fecal contaminants on apples from visible/near infrared hyperspectral reflectance imaging," Journal of Food Engineering, Vol. 81, pp. 412-418 (2007) https://doi.org/10.1016/j.jfoodeng.2006.11.018
  8. B. K. Cho, I. S. Beak, N. G. Lee and C. H. Mo, "Study on bruise detection of 'Fuji' apple using hyperspectral reflectance imagery," Journal of Biosystems Engineering, Vol. 36, pp. 484-490 (2011) https://doi.org/10.5307/JBE.2011.36.6.484
  9. W. S. Kang, "Nondestructive Determination of Seed Viability by Optical Methods," Degree of Doctor of Philosophy, Ph. D. Thesis. Daegu University (2008)
  10. B. K. Cho, Y. R. Chen and M. S. Kim, "Multispectral detection of organic residues on poultry processing plant equipment based on hyperspectral reflectance imaging technique," Computers and Electronics in Agriculture, Vol. 57, pp. 177-189 (2007) https://doi.org/10.1016/j.compag.2007.03.008
  11. M. S. Kim, Y. R. Chen and P. M. Mehl, "Hyperspectral reflectance and fluorescence imaging system for food quality and safety," Transactions of the ASAE, Vol. 44, pp. 721-721 (2001)
  12. J. G. Lim, S. W. Kang, K. J. Lee, C. Y. Mo and J. Y. Son, "Identification of foreign objects in soybeans using near-infrared spectroscopy," Journal of Food Engineering Progress, Vol. 15, pp. 136-142 (2011)
  13. A. Hoskuldsson, "PLS regression methods," Journal of Chemometrics, Vol. 2, pp. 211-228 (1988) https://doi.org/10.1002/cem.1180020306
  14. H. I. Chung and H. J. Kim, "Near-infrared spectroscopy: principles," Journal of Korean Analytical Science & Technology, Vol. 13, No. 1, pp. 1A-14A (2000)

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