Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Monitoring of 7 mycotoxins in pork

돼지고기에서 7종 mycotoxins 잔류실태 조사

  • Kim, Yoen-Joo (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Mi-Ran (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Choi, Tae-Suk (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Young-Seob (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Lee, Ju-Hyoung (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 김연주 (서울특별시보건환경연구원) ;
  • 김미란 (서울특별시보건환경연구원) ;
  • 최태석 (서울특별시보건환경연구원) ;
  • 김영섭 (서울특별시보건환경연구원) ;
  • 이주형 (서울특별시보건환경연구원)
  • Received : 2013.07.30
  • Accepted : 2013.12.10
  • Published : 2013.12.30
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Abstract

This study was conducted to determine the content of 7 mycotoxins (aflatoxin $B_1$, $B_2$, $G_1$, $G_2$, $M_1$, ochratoxin A and zearalenone) using LC-MS/MS in pork available on the Korean markets. The analysis was carried out using following conditions; C18 column ($2.1{\times}100mm$, $1.7{\mu}m$), mobile phase composed of $H_2O$ (0.1 mM $NH_4Ac$ 0.01% HCOOH) : Methanol (0.1 mM $NH_4Ac$ 0.01% HCOOH), binary pump at a flow rate of 0.5 mL/min and $2{\mu}L$ of injection volume, MS/MS detector with ESI positive and negative mode. The quantication of mycotoxins was based on matrix-matched calibration curves with a correlation coefficient in excess of 0.99 for the 7 mycotoxins. The dectection limits were ranged 0.74~2.13 ng/g, with mean recoveries between 73.10~97.46% except aflatoxin $B_1$ (61.31%). We also monitored mycotoxin residues in 208 pork samples. The test results, mycotoxins were not found except one sample. Ochratoxin A in one sample of the test samples was detected below the quantification limit.

Keywords

References

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