The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Antioxidant and Anti-inflammatory Effects of Phellinus linteus HN1009K

목질진흙버섯 Phellinus linteus HN1009K의 항산화 활성과 항염증효과

  • Kang, Hee-Wan (Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Lee, Man Hyee (College of Veterinary Medicine, Gyeongpuk National Univ.) ;
  • Seo, Geon-Sik (Korea National College of Agricultural and Fisheries)
  • 강희완 (한경대학교 미래융합기술대학원) ;
  • 이만휘 (경북대학교 수의과대학) ;
  • 서건식 (한국농수산대학 특용작물학과)
  • Received : 2013.10.22
  • Accepted : 2013.10.23
  • Published : 2013.12.30
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Abstract

Novel Phellinus linteus HN1009K (HK1009) has been characterized on speedy mycelial growth and yearly artificial cultivation. In this study, antioxidant and anti-inflammatory activities of ethanol extract from mycelium and fruiting body of P. linteus HN1009K (HK1009) were compared to those of P. linteus (PL, Korea Sangwhang) and P. baumii (PB, Jangsu Sanghwang) as controls. HK1009 extract showed higher DPPH free radical scavenging activity in both mycelial and fruiting body samples than those of PL and PB. However, in xanthine oxidase assay, antioxidant effect was highly measured on mycelial samples of PL and HK1009. Mycelial extract of HK1009 induced high inhibitory effect on LPS-induced nitric oxide (NO) production in RAW264.7 cells comparing to PL and PB without toxicity on the cells. The results suggest that P. linteus HN1009K possesses superior antioxidant activity and anti-inflammatory effects.

최근 농가에서 재배하고 있는 상황버섯 종에 비하여 균사생장이 빠르고 자실체 발생과 생육이 빠른 P. linteus HN1009K (HK1009)가 개발되었다. 본 연구는 HK1009의 균사체와 자실체 에탄올 추출물의 항산화 및 항염증효과를 기존에 재배되고 있는 P. linteus (PL, Korea Sangwhang)과 P. baumii (PB, Jangsu Sanghwang)를 대조구로 하여 비교분석 하였다. HK1009는 균사체와 자실체 추출물 모두 PL과 PB에 비하여 항산화 활성이 높았으며 xanthine oxidase분석에서는 PL, HK1009, PB순으로 항산화 활성이 높게 나타났다. RAW264.7세포를 이용한 상황버섯 균사체 추출물 PB(EX), PL(EX), HK1009(EX)시료의 NO assay 결과는 PB, PL, HK1009 모두 농도 의존적으로 원액 $200{\mu}g/ml$ 에서는 $7{\sim}8{\mu}M$ 정도로 염증 억제 효과가 뛰어났다. RAW 264.7 세포에 대한 MTT assay 결과에서 PB(EX), PL(EX), HK1009(EX) 추출물의 원액처리구 에서도 세포의 생존능이 70% 이상을 유지하여 세포 독성이 없는 것으로 판단되었다.

Keywords

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