Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Whitening Effect of Biochanin A

Biochanin A의 미백 효과에 관한 연구

  • Park, Sung Ha (Bio Materials R&D center, Kolmar Korea, CBTP, Future Convergent Tch Center) ;
  • Kim, A Hyun (Bio Materials R&D center, Kolmar Korea, CBTP, Future Convergent Tch Center) ;
  • Park, Byoung Jun (Bio Materials R&D center, Kolmar Korea, CBTP, Future Convergent Tch Center) ;
  • Kim, Jin Jun (Bio Materials R&D center, Kolmar Korea, CBTP, Future Convergent Tch Center)
  • Received : 2013.06.25
  • Accepted : 2013.10.11
  • Published : 2013.12.31
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Abstract

To develop a new whitening agent for cosmetics, we investigated the inhibitory effects of biochanin A on melanogenesis. The biochanoin A is an active component in the extracts of Dalbergia odorifera T. Chen. It reduced melanin contents of B16F1 melanoma cells in a dose-dependent manner and decreased to about 48% at a concentration 10 ${\mu}g/mL$. It also inhibited the expression in microphthalmia associated transcription factor (MITF), Tyrosinase, Tyrosinase related protein 1 (Trp-1), and Tyrosinase related protein 2 (Trp-2) in melanocytes. The results suggested that biochanin A has considerable potential as a cosmetics ingredient with a whitening effect.

본 연구는 미백 소재 개발을 위하여 강진향(Dalbergia odorifera T. Chen) 추출물에서 분리한 활성물질인 biochanin A의 멜라닌 생성에 연관된 효과를 알아보았다. Biochanin A는 B16F1 melanoma 세포에서 농도 의존적으로 멜라닌 양을 억제하였으며, 멜라닌 생합성 저해 효과는 10 ${\mu}g/mL$ 농도에서 멜라닌 생성 억제율이 48%로 나타났다. Western blot을 이용하여 microphthalmia associated transcription factor (MITF), Tyrosinase, Trp-1와 Trp-2의 발현을 억제함을 확인하였다. 따라서 biochanin A는 미백 효능을 갖는 화장품 소재로서의 개발 가능성이 클 것으로 기대된다.

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References

  1. H. J. Mu, The effects of polydatin isolated from polygonum cuspidatum on melanogenesis and wrinkle formation, J. Soc. Cosmet. Scientists Korea, 37(4), 327 (2011).
  2. J. S. Chen, C. Wei, and M. R. Marxhal, Inhibition mechanism of kojic acid on polyphenol oxidase, J. Agr. Food Chem., 58, 79 (1991).
  3. K. Urabe, P. Aroca, K. Tsukamoto, D. Mascagna, A. Paulumbo, G. Prota, and V. J. Hering, The inherent cytotoxicty of melanin precursors, Biochim. Biophy. sActa., 1221, 272 (1994). https://doi.org/10.1016/0167-4889(94)90250-X
  4. R. Busca and R. Ballotti, Cyclic AMP a key messenger in the regulation of skin pigmentation, Pigm. Cell Res., 13, 60 (2000). https://doi.org/10.1034/j.1600-0749.2000.130203.x
  5. C. P. Sassone, Coupling gene expression to cAMP signalling: Role of CREB and CREM, Int. J. Biochem. Cell B, 30, 27 (1998). https://doi.org/10.1016/S1357-2725(97)00093-9
  6. B. Saha, Activation of the Mitf promoter by lipid- stimulated activation of p38-stress signalling to CREB, Pigm. Cell Res., 29, 595 (2006).
  7. E. Y. Hwang, A Study on the depigmenting effect of carthamus tinctorius seed, cyperus rotundus and schizonepeta tenuifolia extracts, Korean J. Food Sci. Technol., 44(1), 76 (2012). https://doi.org/10.9721/KJFST.2012.44.1.076
  8. W. C. Ko, Biochanin A, a phytoestrogenic isoflavone with selective inhibition of phosphodiesterase 4, suppresses ovalbumin-induced airwayn hyperresponsiveness, Evidence-Based Complementary and Alternative Medicine, 2011, 13 (2011).
  9. V. C. Lin, In vitro and in vivo melanogenesis inhibition by biochanin a from Trifolium pratense, Biocsi. Biotechnol. Biochem., 75(5), 914 (2011). https://doi.org/10.1271/bbb.100878