Journal of Korean Ophthalmic Optics Society (한국안광학회지)

The Korean Ophthalmic Optics Society (한국안광학회)
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Synaptic Pattern of NMDA R1 upon the Direction-Selective Retinal Ganglion Cells in Developing Mouse Retina

발생 중 마우스 망막에서 방향특이성 신경절세포의 NMDA R1 수용체의 시냅스 패턴

  • Lee, Jee-Geon (Dept. of Biology, Kyungpook National University) ;
  • Kwon, Oh-Ju (Dept. of Optometry, Busan Institute of Science and Technology) ;
  • Jeon, Chang-Jin (Dept. of Biology, Kyungpook National University)
  • 이지건 (경북대학교 생명과학부 생물학과) ;
  • 권오주 (부산과학기술대학교 보건웰빙학부 안경광학과) ;
  • 전창진 (경북대학교 생명과학부 생물학과)
  • Received : 2013.10.30
  • Accepted : 2013.12.14
  • Published : 2013.12.31
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Abstract

Purpose: To investigate the synaptic pattern of NMDA glutamate receptor subtype NMDA R1 on the dendritic arbors of ON-OFF direction-selective retinal ganglion cells (DS-RGSs) in developing [(5,10) days postnatal (PN)] mouse retina. Methods: ON-OFF DS-RGCs were injected with Lucifer yellow and the cells were identified by their characteristic morphology. To identify glutamatergic excitatory input from bipolar cell, we used a marker for the membrane traffic motor protein kinesin. Results: We identified DS-RGCs in P5, and P10 mouse retina. The immunofluorescence labeling of NMDA R1 was most prominent in the IPL. Our results showed that their presence upon the entire dendritic arbor of ON-OFF DS-RGCs is without any evidence of asymmetry, which would predict direction selectivity. Conclusions: The glutamatergic input from bipolar cell reveals symmetry pattern in all periods of P5, and P10. The results may suggest that direction selectivity not lies in the specific pattern of NMDA R1 receptors.

목적: 본 연구에서는 발생 중[태어난 지 5일(P5), 10일(P10)] 마우스 망막의 ON-OFF 방향특이성 신경절세포 수상돌기 상에서 NMDA R1 수용체의 시냅스 패턴을 연구하고자 하였다. 방법: ON-OFF 방향특이성 신경절세포는 Lucifer yellow를 주사하여 형태학적 특징으로 동정하였다. 이극세포로부터의 글루타메이트의 흥분성 유입을 확인하기 위해 membrane traffic motor 단백질 마커인 kinesin을 이용하였다. 결과: 본 연구에서 P5, P10의 ON-OFF 방향특이성 신경절세포를 동정할 수 있었으며, NMDA R1의 면역반응반점은 내망상층에서 강하게 나타났다. ON-OFF 방향특이성 신경절세포의 수상돌기상의 수용체의 분포패턴에서 방향특이성을 예측할 수 있는 어떠한 비대칭성도 발견하지 못하였다. 결론: 이극세포로부터의 글루타메이트성 자극유입은 P5, P10 단계에서 모두 균형적으로 유입되며, 방향특이성은 NMDA R1 수용체의 특이적인 패턴에 있지 않음을 밝혔다.

Keywords

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