International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Effect of Silk Fibroin Hydrolysate on the Apoptosis of MCF-7 human Breast Cancer Cells

  • Chon, Jeong-Woo (Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Jo, Yoo-Young (Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Kwang-Gill (Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Heui-Sam (Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Yeo, Joo-Hong (Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kweon, HaeYong (Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, RDA)
  • Received : 2013.10.07
  • Accepted : 2013.11.10
  • Published : 2013.12.31
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Abstract

Breast cancer is one of the most common cancers among women worldwide. Recently anticancer agents have been developed using natural substances. To evaluate the anticancer effect of hydrolysates of silk fibroin (HSF), we investigated the effect of HSF on cell viability and apoptosis of a breast cancer cell line, MCF-7, induced through the mitochondrial pathway. The result showed that HSF decreased cell viability in MCF-7 cells in a dose- and time-dependent manner, resulting in an increase in the sub-G1 phase cell population. HSF increased the level of the pro-apoptotic Bax protein and decreased the levels of the anti-apoptotic Bcl-2 protein. In addition, HSF induced apoptosis in MCF-7 cells through a mitochondria-dependent pathway by increasing levels of cytochtome c, and cleavage of PARP. Taken together, these findings suggest that HSF inhibits the proliferation of MCF-7 breast cancer cells through a mitochondria and caspase dependent apoptotic pathway.

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