The Journal of the Society of Korean Medicine Diagnostics (대한한의진단학회지)

The Society of Korean Mecicine Diagnostics (대한한의진단학회)
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Anti-Inflammatory Effect of Extracts from Ligustrum obtusifolium S. fruits in RAW 264.7 Macrophages

RAW 264.7 대식세포 내에서 남정목 열매 추출물의 항염증 효과

  • Moon, Ju-Ho (Dept. of Internal Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Go, Heung (Dept. of Internal Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Shin, Seon-Mi (Dept. of Internal Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Ki-Tae (Dept. of Internal Medicine, College of Oriental Medicine, Se-Myung University)
  • 문주호 (세명대학교 한의과대학 내과학교실) ;
  • 고흥 (세명대학교 한의과대학 내과학교실) ;
  • 신선미 (세명대학교 한의과대학 내과학교실) ;
  • 김기태 (세명대학교 한의과대학 내과학교실)
  • Received : 2013.11.29
  • Accepted : 2013.12.24
  • Published : 2013.12.30
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Abstract

Objectives This study was designed to investigate the anti-inflammatory effect of extracts from Ligustrum obtusifolium S. fruits(LOF) in RAW 264.7 Macrophages stimulated with lipopolysaccharide(LPS). Methods We examined productions of nitric oxide(NO), reactive oxygen species(ROS), inducible isoforms of NO synthase(iNOS), cyclooxygenase-2(COX-2) to investigate the anti-inflammatory effect of LOF extracts. In addition, we measured generation of pro-inflammatory cytokines(TNF-${\alpha}$, IL-6). Results Cell viability showed that LOF extracts had no cytotoxicity in Raw 264.7 cells. The treatment with LOF extracts significantly decreased the generation of NO and pro-inflammatory cytokines(TNF-${\alpha}$, IL-6) in LPS-stimulated macrophage cells. Furthermore LOF extracts inhibited intracellular ROS generation dose dependently and reduced the expression of iNOS, COX-2 proteins. Conclusions These results showed that the LOF extracts had an anti-inflammatory effect on LPS-stimulated Raw 264.7 cells. These findings provide scientific support for the use of this Ligustrum obtusifolium S. for inflammatory-related diseases.

Keywords

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