Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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New Insights into 4-Amino-2-tri-fluoromethyl-phenyl Ester Inhibition of Cell Growth and Migration in the A549 Lung Adenocarcinoma Cell Line

  • Wang, Hao (Department of Respiratory Medicine, the First Affiliated Hospital, Anhui Medical University) ;
  • Gui, Shu-Yu (Department of Respiratory Medicine, the First Affiliated Hospital, Anhui Medical University) ;
  • Chen, Fei-Hu (School of Pharmacy, Anhui Medical University) ;
  • Zhou, Qing (Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University) ;
  • Wang, Yuan (Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University)
  • Published : 2013.12.31
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Abstract

Objective: The present study was designed to investigate the probable mechanisms of synthetic retinoid 4-amino-2-tri-fluoromethyl-phenyl ester (ATPR) inhibition of the proliferation and migration of A549 human lung carcinoma cells. Materials and Methods: After the A549 cells were treated with different concentrations of ATPR or all-trans retinoic acid (ATRA) for 72 h, scratch-wound assays were performed to assess migration. Immunofluorescence was used to determine the distribution of CAV1 and $RXR{\alpha}$, while expression of CAV1, MLCK, MLC, P38, and phosphorylation of MLC and P38 were detected by Western blotting. Results: ATPR could block the migration of A549 cells. The relative migration rate of ML-7 group had significantly decreased compared with control group. In addition, ATPR decreased the expression of a migration related proteins, MLCK, and phosphorylation of MLC and P38. ATPR could also influence the expression of RARs or RXRs. At the same time, CAV1 accumulated at cell membranes, and $RXR{\alpha}$ relocated to the nucleus after ATPR treatment. Conclusions: Caveolae may be implicate in the transport of ATPR to the nucleus. Change in the expression and distribution of $RXR{\alpha}$ may be implicated in ATPR inhibition of A549 cell proliferation. The mechanisms of ATPR reduction in A549 cell migration may be associated with expression of MLCK and phosphorylation of MLC and P38.

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References

  1. Arafat K, Iratni R, Takahashi T, et al (2013). Inhibitory effects of salinomycin on cell survival, colony growth, migration, and invasion of human non-small cell lung cancer A549 and LNM35: Involvement of NAG-1. PLoS One, 8, e66931. https://doi.org/10.1371/journal.pone.0066931
  2. Beardsley A, Fang K, Mertz H, et al (2005). Loss of caveolin-1 polarity impedes endothelial cell polarization and directional movement. J Biol Chem, 280, 3541-7. https://doi.org/10.1074/jbc.M409040200
  3. Brabender J, Metzger R, Salonga D, et al (2005). Comprehensive expression analysis of retinoic acid receptors and retinoid X receptors in non-small cell lung cancer: implications for tumor development and prognosis. Carcinogenesis, 26, 525-30.
  4. Chen C, Diao D, Guo L, et al (2012). All-trans-retinoic acid modulates ICAM-1 N-glycan composition by influencing GnT-III levels and inhibits cell adhesion and transendothelial migration. PLoS One, 7, e52975. https://doi.org/10.1371/journal.pone.0052975
  5. Chew TL, Wolf WA, Gallagher PJ, et al (2002). A fluorescent resonant energy transfer-based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows. J Cell Biol, 156, 543-53. https://doi.org/10.1083/jcb.200110161
  6. Fei ZH, Yao CY, Yang XL, et al (2013). Serum BMP-2 upregulation as an indicator of poor survival in advanced non-small cell lung cancer patients. Asian Pac J Cancer Prev, 14, 5293-9. https://doi.org/10.7314/APJCP.2013.14.9.5293
  7. Gui SY, Chen FH, Zhou Q, and Wang Y (2011). Effects of novel all-trans retinoic acid retinamide derivatives on the proliferation and apoptosis of human lung adenocarcinoma cell line A549 cells. Yakugaku Zasshi, 131, 1465-72. https://doi.org/10.1248/yakushi.131.1465
  8. Hatoum A, El-Sabban ME, Khoury J, et al (2001). Overexpression of retinoic acid receptors alpha and gamma into neoplastic epidermal cells causes retinoic acid-induced growth arrest and apoptosis. Carcinogenesis, 22, 1955-63. https://doi.org/10.1093/carcin/22.12.1955
  9. Huang ME, Ye YC, Chen SR, et al (1988). Use of all-trans retinoic acid in the treatment of acute promyelocytic leukemia. Blood, 72, 567-72.
  10. Isshiki M, Ando J, Yamamoto K, et al (2002). Sites of Ca(2+) wave initiation move with caveolae to the trailing edge of migrating cells. J Cell Sci, 115, 475-84.
  11. Je HD, Gallant C, Leavis PC, and Morgan KG (2004). Caveolin-1 regulates contractility in differentiated vascular smooth muscle. Am J Physiol Heart Circ Physiol, 286, H91-8. https://doi.org/10.1152/ajpheart.00472.2003
  12. Jemal A, Siegel R, Ward E, et al (2008). Cancer statistics, 2008. CA Cancer J Clin, 58, 71-96. https://doi.org/10.3322/CA.2007.0010
  13. Kolega J (2003). Asymmetric distribution of myosin IIB in migrating endothelial cells is regulated by a rho-dependent kinase and contributes to tail retraction. Mol Biol Cell, 14, 4745-57. https://doi.org/10.1091/mbc.E03-04-0205
  14. Lentini D, Guzzi F, Pimpinelli F, et al (2008). Polarization of caveolins and caveolae during migration of immortalized neurons. J Neurochem, 104, 514-23.
  15. Lin LM, Li BX, Xiao JB, et al (2005). Synergistic effect of alltrans-retinoic acid and arsenic trioxide on growth inhibition and apoptosis in human hepatoma, breast cancer, and lung cancer cells in vitro. World J Gastroenterol, 11, 5633-7. https://doi.org/10.3748/wjg.v11.i36.5633
  16. Luo XM, and Ross AC (2006). Retinoic acid exerts dual regulatory actions on the expression and nuclear localization of interferon regulatory factor-1. Exp Biol Med, 231, 619-31. https://doi.org/10.1177/153537020623100517
  17. Mercier I, Jasmin JF, Pavlides S, et al (2009). Clinical and translational implications of the caveolin gene family: lessons from mouse models and human genetic disorders. Lab Invest, 89, 614-23. https://doi.org/10.1038/labinvest.2009.23
  18. Nomoto M, Takeda Y, Uchida S, et al (2012). Dysfunction of the RAR/RXR signaling pathway in the forebrain impairs hippocampal memory and synaptic plasticity. Mol Brain, 5, 1-15. https://doi.org/10.1186/1756-6606-5-1
  19. Ocadiz-Delgado R, Castaneda-Saucedo E, Indra AK, et al (2012). RXRalpha deletion and E6E7 oncogene expression are sufficient to induce cervical malignant lesions in vivo. Cancer Lett, 317, 226-36. https://doi.org/10.1016/j.canlet.2011.11.031
  20. Pandey KK, and Batra SK (2003). RXRalpha: a novel target for prostate cancer. Cancer Biol Ther, 2, 185-6. https://doi.org/10.4161/cbt.2.2.282
  21. Parat MO, Anand-Apte B, and Fox PL (2003). Differential caveolin-1 polarization in endothelial cells during migration in two and three dimensions. Mol Biol Cell, 14, 3156-68. https://doi.org/10.1091/mbc.E02-11-0761
  22. Parton RG, and Simons K (2007). The multiple faces of caveolae. Nat Rev Mol Cell Biol, 8, 185-94. https://doi.org/10.1038/nrm2122
  23. Pitchakarn P, Chewonarin T, Ogawa K, et al (2013). Ellagic Acid inhibits migration and invasion by prostate cancer cell lines. Asian Pac J Cancer Prev, 14, 2859-63. https://doi.org/10.7314/APJCP.2013.14.5.2859
  24. Schmidt JT, Morgan P, Dowell N, and Leu B (2002). Myosin light chain phosphorylation and growth cone motility. J Neurobiol, 52, 175-88. https://doi.org/10.1002/neu.10083
  25. Stunnenberg HG (1993). Mechanisms of transactivation by retinoic acid receptors. Bioessays, 15, 309-15. https://doi.org/10.1002/bies.950150504
  26. Sun XH, Flynn DC, Castranova V, et al (2007). Identification of a novel domain at the N terminus of caveolin-1 that controls rear polarization of the protein and caveolae formation. J Biol Chem, 282, 7232-41. https://doi.org/10.1074/jbc.M607396200
  27. Tsiftsoglou AS, Bonovolias ID, and Tsiftsoglou SA (2009). Multilevel targeting of hematopoietic stem cell selfrenewal, differentiation and apoptosis for leukemia therapy. Pharmacol Ther, 122, 264-80. https://doi.org/10.1016/j.pharmthera.2009.03.001
  28. Urra H, Torres VA, Ortiz RJ, et al (2012). Caveolin-1-enhanced motility and focal adhesion turnover require tyrosine-14 but not accumulation to the rear in metastatic cancer cells. PLoS One, 7, e33085. https://doi.org/10.1371/journal.pone.0033085
  29. Vyas A, Patitungkho S, Jamadar A, et al (2012). ATRAhydrazonate derivatives and their copper complexes against hormone-dependent (MCF-7), hormone-independent (MDA-MB-231and BT-20) breast cancer and androgenindependent (PC3) prostate cancer cell lines. Inorganic Chemistry Communications, 23, 17-20. https://doi.org/10.1016/j.inoche.2012.05.027
  30. Wadgaonkar R, Nurmukhambetova S, Zaiman AL, and Garcia JG (2003). Mutation analysis of the non-muscle myosin light chain kinase (MLCK) deletion constructs on CV1 fibroblast contractile activity and proliferation. J Cell Biochem, 88, 623-34. https://doi.org/10.1002/jcb.10370
  31. Wan YJ, An D, Cai Y, et al (2000). Hepatocyte-specific mutation establishes retinoid X receptor alpha as a heterodimeric integrator of multiple physiological processes in the liver. Mol Cell Biol, 20, 4436-44. https://doi.org/10.1128/MCB.20.12.4436-4444.2000
  32. Wan YJ, Cai Y, and Magee TR (1998). Retinoic acid differentially regulates retinoic acid receptor-mediated pathways in the Hep3B cell line. Exp Cell Res, 238, 241-7. https://doi.org/10.1006/excr.1997.3851
  33. Wang B, Yan Y, Zhou J, et al (2013). A novel all-trans retinoid acid derivatives inhibits the migration of breast cancer cell lines MDA-MB-231 via myosin light chain kinase involving p38-MAPK pathway. Biomed Pharmacother, 67, 357-62. https://doi.org/10.1016/j.biopha.2013.03.016
  34. Wang M, Zhao JJ, Gui SY, et al (2012). Effect of all-trans retinoic acid and its derivative 4-amino-2-trifluoromethylphenyl ester on proliferation inhibition of A549 cells and its correlation with the expression of osteopontin. Acta Universitatis Medicinalis Anhui, 47, 1312-6. (in Chinese).
  35. Watters KM, Bryan K, Foley NH, et al (2013). Expressional alterations in functional ultra-conserved non-coding RNAs in response to all-trans retinoic acid--induced differentiation in neuroblastoma cells. BMC Cancer, 13, 184. https://doi.org/10.1186/1471-2407-13-184
  36. Yamada E (1955). The fine structure of the gall bladder epithelium of the mouse. J Biophys Biochem Cytol, 1, 445-58. https://doi.org/10.1083/jcb.1.5.445
  37. Ye X, Wu Q, Liu S, et al (2004). Distinct role and functional mode of TR3 and RARalpha in mediating ATRA-induced signalling pathway in breast and gastric cancer cells. Int J Biochem Cell Biol, 36, 98-113. https://doi.org/10.1016/S1357-2725(03)00143-2
  38. Zhong C, Yang S, Huang J, et al (2003). Aberration in the expression of the retinoid receptor, RXRalpha, in prostate cancer. Cancer Biol Ther, 2, 179-84. https://doi.org/10.4161/cbt.2.2.281

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