Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Survival Rates with Time Course of Frozen-thawed Pacific Oyster Larvae in Indoor Rearing System

  • Kim, Ki Tae (Department of Marine Bio-materials and Aquaculture, Pukyong National University) ;
  • Lim, Han Kyu (Department of Fisheries and Marine Resources, Mokpo National University) ;
  • Chang, Young Jin (Department of Marine Bio-materials and Aquaculture, Pukyong National University)
  • Received : 2013.11.14
  • Accepted : 2013.12.04
  • Published : 2013.12.31
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Abstract

Post-thawed larval rearing in Pacific oyster Crassostrea gigas was performed to investigate the survival rate with time course in three kinds of larvae cryopreserved. The highest survival rate and larval activity index (LAI) of post-thawed larvae were obtained from the permeation in 0.2 M sucrose and 2.0 M ethylene glycol (EG) at $-1^{\circ}C/min$ in freezing speed showing the survival rates just after thawing of 63.8% in trochophore, 84.1% in D-shaped veliger and 56.3% in early umbo veliger. In post-thawed larval rearing with food supply, the larvae lasted their lives until 24 hours in trochophore, 75 hours in D-shaped veliger and 57 hours in early umbo veliger. The results suggested that each larval stage post-thawed revealed no more further development to subsequent respective stage.

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References

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