Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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SCAR markers were developed to identify zoysiagrass mutants exhibiting fine leaf characteristics

세엽 한국들잔디 변이체 식별을 위한 SCAR 마커 개발

  • Chung, Sung Jin (Department of Horticulture, Chungnam National University) ;
  • Park, Su Jeong (Department of Horticulture, Chungnam National University) ;
  • Choi, Young In (Department of Horticulture, Chungnam National University) ;
  • Kim, In-Kyung (Department of Horticulture, Chungnam National University) ;
  • Lee, Ka-Yeon (Department of Horticulture, Chungnam National University) ;
  • Kim, Hun-Joong (Department of Horticulture, Chungnam National University) ;
  • Lee, Geung-Joo (Department of Horticulture, Chungnam National University)
  • Received : 2013.06.04
  • Accepted : 2013.06.17
  • Published : 2013.06.30
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Abstract

Polymorphic bands of two fine-leaf zoysiagrass mutants (CNU 70-1, CNU 70-2) induced via a gamma-ray irradiation on seeds of Zoysia japonica were obtained by using randomly amplified polymorphic DNA (RAPD) primers. The genotype-specific fragments were then converted into PCR-based sequence characterized amplified region (SCAR) markers, which are now amenable to detecting them among other zoysiagrass species widely noticeable in Korea. The CNU 70-1-specific primer set amplified about 900 bp successfully, while the CNU 70-6 marker produced the expected 1,500 bp band, by which those markers were nominated by CNU 70-1_900 and CNU 70-6_1500 SCARs, respectively. The developed SCAR markers can be an applicable tool in sod industry where illegal appropriation hampers breeder's right and profits due to the turfgrass plant vegetatively propagating.

Keywords

References

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