The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Plantaginis Semen & Poria (CJB) Water Extract on Histomorphometrical Aspects and Histopathological Changes in High Fat Diet-induced Obese Rats

차전자·복령의 복합 물추출물(CJB)이 고지방사료로 유도된 비만 생쥐의 조직형태 및 조직병리학적 변화에 미치는 영향

  • Hwang, Jeong Soo (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Suk, Jang Mi (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Choi, Hye min (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Shin, Insoon (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Hwang, Su Jung (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Park, Ji Young (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Kim, Sung Ok (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Seo, Bu Il (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Haany University)
  • 황정수 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 석장미 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 최혜민 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신인순 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 황수정 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 박지영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김성옥 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 서부일 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2012.12.13
  • Accepted : 2013.01.16
  • Published : 2013.01.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Obesity is a chronic metabolic disease caused by disorder of energy balance and lipid metabolism. This study was conducted by histopathology and histomorphometry to investigate the anti-obesity effects of mixed water extract of Plantaginis Semen & Poria (CJB) on liver, epididymal fat pads and pancreas zymogen granules in obese rats induced with high fat diet. Method : Male Sprague-Dawley rats to be divided four groups were fed into four different treatments: normal (NOR) diet, high-fat (HF) diet, HF diet+CJB (100 mg/kg and 300 mg/kg, P.O.) for 8 weeks. The weekly body weights were measured in four experimental groups, respectively. Also histopathological and histomorphometrical changes of liver, epididymal fat pads and pancreas zymogen granules were observed in normal control and obese rats, respectively. Results : Adminstration of CJB significantly reduced body weights compared to those of HF group for experimental period. After 8 weeks, liver weights in the CJB groups were lower than those of HF group. In addition, HF diet related steatohepatitis, adipocyte hypertrophy, exocrine disturbances (decreases of pancreatic zymogen granules) were also dose-dependently inhibited by treatment of test material, CJB 100 and CJB 300 as compared with HF group, respectively. Conclusion : Based on the results, it is considered that CJB will be showed hepatoprotective and anti-obese effects, may be directly and/or indirectly mediated by pancreatic zymogen granules because they dose-dependently inhibited steatohepatitis, hypertrophy of adipocytes and decreases of pancreatic zymogen granules induced by HF diet supply, respectively.

Keywords

References

  1. Korea Center for Disease Control and Prevention. Retrieved aug 20, 2012, from : http : // www.cdc.go.kr/ CDC/health/CdcKrHealth0101.jsp?menuIds=HOME001-MNU0001-MNU0754-MNU0756 &fid=790&cid=18021. In;2007.
  2. Seo BI, Lee JH, Cho HY, Kwon DR, Bu YM. Hanyakbonchohak. 1nd rev. ed. Seoul : Younglimsa. 2006 : 413-5.
  3. Kang JH. Evaluation criteria of healthy body weight in Koreans - focus on obesity. Korean J Nutr. 2001 ; 6 : 397-401.
  4. Lee HJ, Chung MJ, Kim DJ, Choe M. Effects of Oenanthe javanica, Coicis lachryma-jobi L. var., and Plantaginis asiatica L. Water Extracts on Activities of Key Enzymes on Lipid Metabolism. J Korean Soc Food Sci Nutr . 2009 ; 38(11) : 1516-21. https://doi.org/10.3746/jkfn.2009.38.11.1516
  5. Cho SY, Kim MJ. The effect of plantaginis semen on serum and hepatic lipid metabolism in fed high and low fat diets. J Korean Soc Food Sci Nutr . 1995 ; 24 : 517-22.
  6. Sato M, Tai T, Nunoura Y, Yajima Y, Kawashima S, Tanaka K. Dehydrotra-metenolic acid induces preadipocyte differentiation and sensitizes animal models of noninsulin-dependent diabetes mellitus to insulin. Biol Pharm Bull. 2000 ; 25(1) : 81-6.
  7. Kaminage T, Yasukawa K, Kanno H, Tai T, Nunoura Y, Takido M. Inhibitory effects of lanostane-type triterpene acids, the components of Poria cocos, on tumor promotion by 12-Otetradecanoylphorbol- 13-acetate in two-stage carcinogenesis in mouse skin. Oncology. 1996 ; 53(5) : 382-5. https://doi.org/10.1159/000227592
  8. Nukaya H, Yamashiro H, Fukazawa H, Ishida H, Tsuji K. Isolation of inhibitors of TPA-induced mouse ear edema from Hoelen, Poria cocos. Chem Pharm Bull (Tokyo). 1996 ; 44(4) : 847-9. https://doi.org/10.1248/cpb.44.847
  9. Yun HJ, Cha HM, Kim SW, Shin WC, Kim HG, Choe SY. Effects of the Extract of Hoelen on Serum Lipid Profiles in Mice. J Korean Soc Food Sci Nutr. 2006 ; 35(8) : 1005-9. https://doi.org/10.3746/jkfn.2006.35.8.1005
  10. American institute of nutrition. Report of the American institute of nutrition. Ad Hoc Committee on standards for nutritional studies. Nutrition. 1977 ; 107 : 1340-8.
  11. Research NBoE. The Increasing Prevalence of Obesity. 2011. Retrieved aug 26, 2012, from : http : //www.nber.org
  12. 2008 Korean National Health and Nutrition Examination Survey. Retrieved sep 3, 2012, from : http://stat.mw.go.kr/front/statData/publicationView .jsp?menuId=47&bbsSeq=13&nttSeq=14713&searchKey=&searchWord=&nPage=3
  13. Mokdad AH, Serdula MK, Dietz WH, Bowman BA, Marks JS, Koplan JP. The spread of the obesity epidemic in the United States, 1991-1998. JAMA. 1999 ; 282(16) : 1519-22. https://doi.org/10.1001/jama.282.16.1519
  14. Heo J. Donguibogam. Seoul : Bubin publishers. 2005 : 119,334,1145.
  15. Jang GB. Kyungakjunsu. 1nd rev. ed. Seoul : Deasungmunhwasa. 1988 : 638.
  16. Lim JP. Herb Medicinal Pharmacognosy. 3nd rev. Seoul : Shinilbooks. 1992 : 193.
  17. Cho SY, Kin MJ. The effect of plantanginis semen on serum and hepatic lipid metabolism in fed high and low fat diets. J Korean Soc Food Sci Nutr. 1995 ; 24 : 517-22.
  18. Chung IM, Yun HS, Kim YS, Ahn JW. Aucubin protential antidote for $\alpha$-amanitin poisoning. J Toxicol Clin Toxicol. 1984 ; 22 : 77-85. https://doi.org/10.3109/00099308409035083
  19. Kim DG, Son DH, Choi UK, Cho YS, Kim SM. The antioxidant ability and nitrite scavenging ability of Poria cocos. J Korean Soc Food Sci Nutr. 2002 ; 31 : 1097-101. https://doi.org/10.3746/jkfn.2002.31.6.1097
  20. Hattori T, Hayashi K, Nagao T, Furuta K, Ito M, Suzuki Y. Studies on antinephritic effects of plant components (3) Effect of pachyman, a maincomponent of poria cocos Wolf on original-type anti-GBM nephritis in rats and its mechanisms. Jpn J Pharmacol. 1992 ; 59 : 89-91. https://doi.org/10.1254/jjp.59.89
  21. Choi S, Choi HS, Kim JY, Jang YG, Kim SY, Park MH, Hong SG, Hwang SJ. The effects of Very Low Calorie Diet using meal replacements that contain Herbal extracts on weight reduction and health promotion. J Korean Ori Asso Stu Obe. 2004 ; 4(1) : 81-94
  22. Yoo SJ. Pharmacological treatment of obesity. Kor J Endocrine Society. 2008 ; 23 : 223-33.
  23. Hwang JS, Suk JM, Choi HM, Shin IS, Hwang SJ, Park JY, Kim SO, Seo BI, Kim MR. Effects of CJB Water Extract on Obesity-Related Factors in Hypothalamus of Rats Fed High-Fat Diet. Kor J Herbology. 2012 ; 27(5) : 99-107. https://doi.org/10.6116/kjh.2012.27.5.99
  24. Lowe ME. Molecular mechanisms of rat and human pancreatic triglyceride lipases. Nutrition. 1997 ; 127 : 549-57.
  25. Lowe ME. The triglyceride lipases of the pancreas. J Lipid Res. 2002 ; 43 : 2007-16. https://doi.org/10.1194/jlr.R200012-JLR200
  26. Moreno DA, Ilic N, Poulev A, Brasaemle DL, Fried SK, Raskin I. Inhibitory effects of grape seed extract on lipases. Nutrition. 2003 ; 19 : 876-9. https://doi.org/10.1016/S0899-9007(03)00167-9
  27. Ichikawa A. Fine structural changes in response to hormonal stimulation of perfused canine pancreas. J Cell Biol. 1965 ; 24 : 369-85. https://doi.org/10.1083/jcb.24.3.369