Journal of Biomedical and Translational Research

Research Institute of Veterinary Medicine Chungbuk National University (충북대학교 동물의학연구소)
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Characteristics and immuno-modulatory effects of Weissella cibaria JW15 isolated from Kimchi, Korea traditional fermented food, for probiotic use

  • Ahn, Su-Bin (College of Veterinary Medicine, Chungbuk National University) ;
  • Park, Ho-Eun (College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Sang-Myeong (Division of Biotechnology, College of Environmental & Bioresource Sciences, Chonbuk National University) ;
  • Kim, So-Young (Functional Food & Nutrition Division, Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Shon, Mi-Yae (International Ginseng&Herb Research Institute) ;
  • Lee, Wan-Kyu (College of Veterinary Medicine, Chungbuk National University)
  • Received : 2013.10.25
  • Accepted : 2013.11.15
  • Published : 2013.12.31
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Abstract

In this study, characteristics and immuno-modulatory effects of Weissella cibaria JW15 isolated from Kimchi, traditional Korean fermented food, were examined for investigation of the capacity of potentially probiotic strains. We measured acid, bile, and heat tolerance, adhesive properties to intestinal epithelial cells, and inhibitory activity against pathogens. JW15 could survive at pH 3.0 for 2 hr, but not at pH 2.0. JW15 also showed tolerance to 0.3% oxgall bile salt, and heat tolerance at $70^{\circ}C$ and $80^{\circ}C$ for 5 min, respectively. Adhesive ability to Caco-2 cells was similar to that of Lactobacillus rhamnosus GG (LGG), a well-known commercial probiotic. JW15 exhibited antimicrobial activities to pathogenic bacteria such as Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella enteritidis. The immuno-modulatory effects of JW15 were compared with those of LGG, a well-known immune enhancer. For analysis, production of nitric oxide (NO), NF-${\kappa}B$ (Nuclear factor ${\kappa}B$), Interleukin-$1{\beta}$ (IL-$1{\beta}$), and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) was measured. The concentration of NO induced by JW15 was higher than that by LGG at low concentration ($1{\times}10^7$ cfu/mL). Low and high ($5{\times}10^7$ CFU/mL) concentration of JW15 induced statistically higher production of NF-${\kappa}B$, IL-$1{\beta}$, and TNF-${\alpha}$ than that produced by LGG, respectively. In conclusion, Weissella cibaria JW15 had ability as a probiotic strain, including acid, bile, and heat tolerance, adhesive properties to intestinal epithelial cells, and inhibitory activity against pathogens. In addition, JW15 showed better immuno-modulatory effects than LGG when NO, NF-${\kappa}B$, IL-$1{\beta}$, and TNF-${\alpha}$ were measured. According to these results, the characteristics and immunomodulating activity of Weissella cibaria JW15 are suitable for consideration as a potential probiotic.

Keywords

Acknowledgement

Grant : Cooperative Research Program for Agricultural Science & Technology Development

Supported by : Rural Development Administration

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