Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Antioxidant, anti-inflammatory and anti-acetylcholinesterase activities of fruiting bodies of Phellinus xeranticus

기와층버섯 자실체의 메탄올 및 열수추출물의 항산화, 항염증 및 아세틸콜린에스테라제 저해 활성

  • Nguyen, Trung Kien (Division of Life Sciences, Incheon National University) ;
  • Shin, Do Bin (Division of Life Sciences, Incheon National University) ;
  • Lee, Kyung Rim (Division of Life Sciences, Incheon National University) ;
  • Shin, Pyung Gyun (Mushroom Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cheong, Jong Chun (Mushroom Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Yoo, Young Bok (Mushroom Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Lee, Min Woong (Department of Life Sciences, Dongguk University) ;
  • Jin, Ga-Heon (Department of Ophthalmic Optics, Shinheung University) ;
  • Kim, Hye Young (Department of Clinical Laboratory Science, Shinsung University) ;
  • Im, Kyung Hoan (Division of Life Sciences, Incheon National University) ;
  • Lee, Tae Soo (Division of Life Sciences, Incheon National University)
  • ;
  • 신도빈 (인천대학교 생명과학기술대학 생명과학부) ;
  • 이경림 (인천대학교 생명과학기술대학 생명과학부) ;
  • 신평균 (원예특작과학원 버섯과) ;
  • 정종천 (원예특작과학원 버섯과) ;
  • 유영복 (원예특작과학원 버섯과) ;
  • 이민웅 (동국대학교) ;
  • 진가헌 (신흥대학교 안경과학과) ;
  • 김혜영 (신성대학교 임상병리과) ;
  • 임경환 (인천대학교 생명과학기술대학 생명과학부) ;
  • 이태수 (인천대학교 생명과학기술대학 생명과학부)
  • Received : 2013.12.04
  • Accepted : 2013.12.30
  • Published : 2013.12.31
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Abstract

Phellinus xeranticus is an medicinal mushroom belongs to Family Hymenochaetaceae of Polyporales, Basidiomycota. The purpose of this study was to investigate the antioxidant, anti-inflammatory and anti-acetylcholinesterase activities of methanol and hot water extracts prepared from fruiting bodies of Phellinus xeranticus. Besides measuring of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, a reducing power and a chelating activity on ferrous ions were also measured to evaluate the antioxidant activity of the extracts. To measure the anti-inflammatory activities of the extracts, nitric oxide(NO) production from lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage cells and carrageenan-induced acute hind paw edema of rats were investigated. The results showed that the extracts have excellent DPPH scavenging and chelating activity on the ferrous ions compared with positive controls. The nitric oxide (NO) production in LPS-induced RAW 264.7 macrophage cells were decreased as the concentration of the mushroom extracts increased. Significant reduction of paw edema of rats were observed at 2~6 h after treatment of methanol and hot-water extracts with 50 mg/kg concentration to the rats which are induced acute hind paw edema by carrageenan administration. The anti-acetylcholinesterase activity of the methanol extract of the mushroom showed 83.34% inhibition on AcHE which is lower than that of positive control galanthamine. The experimental results suggested that methanol and hot-water extracts of Phellinus xeranticus fruiting bodies might be used for good sources of antioxidant, anti-inflammatory and anti-acetylcholinesterase agents.

본 연구에서는 기와층버섯의 자실체에서 메탄올과 열수를 이용해 추출한 물질의 항산화와 항염증, 항아세틸콜린에스테라제(anti-acetylcholinesterase)의 효과를 탐색하였다. DPPH 라디칼 소거능, 환원력 및 철 이온 제거능 등을 이용해 항산화 효과를 측정한 결과 양성대조군으로 사용한 BHT나 토코페롤에 비해 낮았지만 다른 종류의 버섯에 비해 효과가 우수한 것을 확인하였다. 철 이온을 제거하는 항산화 실험에서 기와층버섯의 메탄올 추출물의 효과는 양성대조군인 BHT나 토코페롤에 비해 월등하게 높아서 기와층버섯 자실체의 추출물은 높은 항산화 효과를 지닌 것으로 나타났다. 기와층버섯의 염증저해 효과 실험에서는 배양 중인 RAW 264.7 대식세포에 자실체의 메탄올과 열수추출물을 각각 전 처리 한 후 염증매개 물질인 LPS를 투여하여 추출물의 NO 생성 저해효과를 조사하였다. 실험 결과, 처리한 추출물의 농도가 증가함에 따라 생성된 NO의 양이 현저하게 감소하는 경향을 나타내었다. 또한 기와층버섯의 추출물을 이용해 carrageenan에 의해 흰쥐 뒷발에 유도된 부종을 저해하는 실험에서는 투여한 추출물의 농도가 증가함에 따라 흰쥐의 뒷발에 유도된 부종의 용적도 농도 의존적으로 감소되었다. 따라서 기와층버섯 자실체에 함유된 항산화, 항염증 및 항아세틸콜린에스테라제 성분은 천연 항산화제, 소염제 및 알츠하이머병 치료에 이용이 가능할 것으로 사료되었다.

Keywords

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