The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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The effect of Astragalus membranaceus methanol extract on hyaluronic acid production in HaCaT cells

황기 메탄올 추출물이 HaCaT 세포에서 Hyaluronic acid 생성에 미치는 영향

  • Lee, Pyeong-Jae (Dept. of Natural Medicine Resources, Semyung University) ;
  • Kim, Hee-Taek (Dept. of Oriental Medical Opthalmology & Otolaryngology & Dermatology, Semyung University Oriental Medical Hospital) ;
  • Yoon, Kyung-Sup (Saimdang Cosmetics Co. Ltd., R&D Center) ;
  • Park, Hyun-Chul (Saimdang Cosmetics Co. Ltd., R&D Center) ;
  • Ha, Hun-Yong (Dept. of Natural Medicine Resources, Semyung University)
  • 이평재 (세명대학교 자연약재과학과) ;
  • 김희택 (세명대학교 한의과대학 안이비인후피부과학교실) ;
  • 윤경섭 (사임당화장품 기술연구소) ;
  • 박현철 (사임당화장품 기술연구소) ;
  • 하헌용 (세명대학교 자연약재과학과)
  • Received : 2013.01.08
  • Accepted : 2013.02.07
  • Published : 2013.02.25
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Abstract

Objectives: Hyaluronic acid, high molecular glycosaminoglycan, exists in extracellular matrix of tissue, especially, in skin and has been known to be deeply involved in skin hydration. In this study, we investigated the effect of methanol extract of Hwang-gi, Astragalus membranaceus root, on hyaluronic acid production in human keratinocyte HaCaT cells. Methods: We determined hyaluronic acid synthase 2 gene expression and hyaluronic acid production in HaCaT cells by using RT-PCR and ELISA, respectively. Results: Hwang-gi extract didn't show the toxicity to HaCaT cells within the treated concentration and increased the hyaluronic acid synthase 2 gene expression and hyaluronic acid production. Conclusions: Hyaluronic acid production increased by Hwang-gi could be, partially, contribute to the moisturing effect in skin by it.

Keywords

References

  1. Verdier-Sevrain S, Bonte F. Skin hydration: a review on its molecular mechanisms. J Cosmet Dermatol. 2007;6(2):75-82. https://doi.org/10.1111/j.1473-2165.2007.00300.x
  2. Ghersetich I, Lotti T, Campanile G, Grappone C, Dini G. Hyaluronic acid in cutaneous intrinsic aging. Int J Dermatol. 1994;33 (2):119-22. https://doi.org/10.1111/j.1365-4362.1994.tb01540.x
  3. Dai G, Freudenberger T, Zipper P, Melchior A, Grether-Beck S, Rabausch B, et al. Chronic ultraviolet B irradiation causes loss of hyaluronic acid from mouse dermis because of down-regulation of hyaluronic acid synthases. Am J Pathol. 2007;171(5):1451-61. https://doi.org/10.2353/ajpath.2007.070136
  4. Stern R, Maibach HI. Hyaluronan in skin: aspects of aging and its pharmacologic modulation. Clin Dermatol. 2008;26(2): 106-22. https://doi.org/10.1016/j.clindermatol.2007.09.013
  5. Sim GS, Lee DH, Kim JH, An SK, Choe TB, Kwon TJ, et al. Black rice (Oryza sativa L. var. japonica) hydrolyzed peptides induce expression of hyaluronan synthase 2 gene in HaCaT keratinocytes. J Microbiol Biotechnol. 2007;17(2):271-79.
  6. Kim S, Kang BY, Cho SY, Sung DS, Chang HK, Yeom MH, et al. Compound K induces expression of hyaluronan synthase 2 gene in transformed human keratinocytes and increases hyaluronan in hairless mouse skin. Biochem Biophys Res Commun. 2004;316 (2):348-55. https://doi.org/10.1016/j.bbrc.2004.02.046
  7. Kim SH, Nam GW, Kang BY, Lee HK, Moon SJ, Chang IS. The Effect of Kaempferol, Quercetin on Hyaluronan-Synthesis Stimulation in Human Keratinocyte (HaCaT). J Soc Cosmet Scientists Korea. 2005;31(1):97-102.
  8. Saavalainen K, Pasonen-Seppänen S, Dunlop TW, Tammi R, Tammi MI, Carlberg C. The human hyaluronan synthase 2 gene is a primary retinoic acid and epidermal growth factor responding gene. J Biol Chem. 2005;280(15):14636-44. https://doi.org/10.1074/jbc.M500206200
  9. Furuya T, Yamagata S, Shimoyama Y, Fujihara M, Morishima N, Ohtsuki K. Biochemical characterization of glycyrrhizin as an effective inhibitor for hyaluronidases from bovine testis. Biol Pharm Bull. 1997;20(9): 973-77. https://doi.org/10.1248/bpb.20.973
  10. Kakegawa H, Matsumoto H, Satoh T. Inhibitory effects of some natural products on the activation of hyaluronidase and their anti-allergic actions. Chem Pharm Bull. 1992;40(6):1439-42. https://doi.org/10.1248/cpb.40.1439
  11. Murata T, Miyase T, Yoshizaki F. Hyaluronidase inhibitors from Keiskea japonica. Chem. Pharm. Bul. 2012;60(1): 121-28. https://doi.org/10.1248/cpb.60.121
  12. Ahn DK. Illustrated Book Of Korean Medicinal Herbs. 3rd ed. Seoul:Kyo-Hak Publishing Co. Ltd. 2000.
  13. Jung TK, Kim MJ, Lim KR, Yoon KS. Moisturing and Anti-oxidation Effect of Astragalus membranaceus. J Soc Cosmet Scientists Korea. 2006;32(3):193-200.
  14. Lee YH, Choi SI, Lee JW, Jung SM, Park SM, Heo TR. Isolation of Hyaluronidase Inhibitory Component from the Roots of Astraglus membranaceus Bunge (Astragali Radix). Food Sci Biotechnol. 2005;14(2):263-67.
  15. Cha BC. Tyrosinase, Hyaluronidase Inhibitory Effect and Antioxidant Activity of Medicinal Plants. Kor J Pharmacogn. 2011;42(1):89-97.
  16. Hsu MF, Chiang BH. Stimulating effects of Bacillus subtilis natto-fermented Radix astragali on hyaluronic acid production in human skin cells. J Ethnopharmacol. 2009; 125(3):474-81. https://doi.org/10.1016/j.jep.2009.07.011
  17. Wu T, Annie Bligh SW, Gu LH, Wang ZT, Liu HP, Cheng XM, et al. Simultaneous determination of six isoflavonoids in commercial Radix Astragali by HPLC-UV. Fitoterapia. 2005;76(2):157-65. https://doi.org/10.1016/j.fitote.2004.11.006
  18. Qi LW, Yu QT, Li P, Li SL, Wang YX, Sheng LH, et al. Quality evaluation of Radix Astragali through a simultaneous determination of six major active isoflavonoids and four main saponins by high-performance liquid chromatography coupled with diode array and evaporative light scattering detectors. J Chromatogr A. 2006;1134(1-2):162-9. https://doi.org/10.1016/j.chroma.2006.08.085
  19. Miyazaki K, Hanamizu T, Iizuka R, Chiba K. Genistein and daidzein stimulate hyaluronic acid production in transformed human keratinocyte culture and hairless mouse skin. Skin Pharmacol Appl Skin Physiol. 2002;15(3):175-83. https://doi.org/10.1159/000063546

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