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Variation of Anthocyanin Content and Estimation of Anthocyanin Content from Colorimeter among Strawberry Accessions

딸기 유전자원의 안토시아닌 함량 변이와 색차계를 이용한 안토시아닌 함량 추정

  • Kim, Dae-Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Sung Kyeom (Department of Plant Science, Seoul National University) ;
  • Chen, Changhoo (Department of Plant Science, Seoul National University) ;
  • Kim, Su (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Chae, Won Byoung (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kwak, Jung-Ho (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Suhyung (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cheong, Seung-Ryong (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoon, Moo Kyung (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • 김대영 (국립원예특작과학원 채소과) ;
  • 김성겸 (서울대학교 식물생산과학부) ;
  • 전창후 (서울대학교 식물생산과학부) ;
  • 김수 (국립원예특작과학원 채소과) ;
  • 채원병 (국립원예특작과학원 채소과) ;
  • 곽정호 (국립원예특작과학원 채소과) ;
  • 박수형 (국립원예특작과학원 채소과) ;
  • 정승룡 (국립원예특작과학원 채소과) ;
  • 윤무경 (국립원예특작과학원 채소과)
  • Received : 2013.10.28
  • Accepted : 2013.11.20
  • Published : 2013.12.31

Abstract

This study was conducted to find out an efficient and non-destructive method to select strawberry plants with high anthocyanin content in breeding programs. The total anthocyanin content in both fruit skin and whole fruit of fully ripe fruits was analyzed by HPLC in 19 strawberry cultivars, which were selected among germplasm as potential breeding materials based on their good horticultural traits. The anthocyanin content varied greatly among cultivars, ranging from 22.04 to 87.16 $mg{\cdot}100g^{-1}$ FW in fruit skin and from 1.98 to 30.16 $mg{\cdot}100g^{-1}$ FW in whole fruit. For the anthocyanin content of whole fruit, it was high in the order of 'Sachinoka', 'Tamar', 'Amaou' and 'Summerberry'. A correlation coefficient of anthocyanin content between fruit skin and whole fruit was significantly high as 0.867 and, therefore, the anthocyanin content of whole fruit can be estimated by that of fruit skin. Besides, the anthocyanin content of whole fruit was strongly negatively correlated with $L^*$ (lightness, -0.791) and $b^*$ (yellowness, -0.772) values measured by portable colorimeter. Therefore, the portable colorimeter can be a rapid and nondestructive method to estimate anthocyanin content of strawberry fruits when selecting anthocyanin-enriched strawberry lines in strawberry breeding programs.

딸기 유전자원 중 원예적 특성이 우수하여 육종 소재로 활용 가능성이 높은 19품종을 대상으로 완숙된 과실을 부위별(과피 및 과실 전체)로 안토시아닌 함량을 분석하고 색차계를 이용하여 비파괴적인 방법으로 안토시아닌 고함유 계통을 선발할 수 있는지에 대하여 검토하였다. 그 결과 딸기의 과피는 22.04~87.16 $mg{\cdot}100g^{-1}$ FW, 과실 전체는 1.98~30.16 $mg{\cdot}100g^{-1}$ FW의 범위에 분포하여 측정 부위와 유전자원에 따라 안토시아닌 함량의 변이가 큼을 알 수 있었다. 과실 전체를 기준으로 하였을 때, 조사된 유전자원 중 'Sachinoka', 'Tamar', 'Amaou', 'Summerberry' 순으로 안토시아닌 함량이 높게 나타났다. 과피와 과실 전체 부위의 안토시아닌 함량간의 상관계수가 0.867로 높아 과피를 기준으로 하여 과실 전체의 안토시아닌 함량을 추정할 수 있을 것으로 생각된다. 또한 과실 전체의 안토시아닌 함량과 측정된 색차계의 $L^*$값(-0.791) 및 $b^*$값(-0.772)에서 높은 역의 상관관계를 보였다. 따라서 향후 딸기 육종 프로그램에서 안토시아닌 고함유 계통을 신속하게 선발할 때, 색차계를 보조적인 방법으로 활용할 수 있을 것으로 판단된다.

Keywords

References

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