Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Calcium Extracted from Salted Anchovy (Engraulis japonicus) on Calcium Metabolism of the Rat

멸치젓에서 추출한 칼슘이 흰쥐의 칼슘대사에 미치는 영향

  • 김향숙 (동의대학교 항노화연구소) ;
  • 최은옥 (동의대학교 항노화연구소) ;
  • 김만도 ((주)지에스피) ;
  • 최영현 (동의대학교 한의학과) ;
  • 김병우 (동의대학교 블루바이오소재개발센터) ;
  • 김수연 (숭의여자대학교 식품영양과) ;
  • 황혜진 (동의대학교 식품영양학과)
  • Received : 2012.12.07
  • Accepted : 2013.01.28
  • Published : 2013.02.28
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Abstract

This study was conducted to examine the effect of calcium extracted from salted anchovy (Engraulis japonicus) on the calcium metabolism of rats. Sprague-Dawley male rats were fed low-calcium diets (0.15%) for 2 weeks after the adjustment period. Rats were divided into five groups and were fed experimental diet for four weeks. Experimental diets were low calcium (LC, 0.15% $CaCO_3$), 0.5% $CaCO_3$ (CC), seaweed calcium (SC), calcium lactate (LC), anchovy calcium (AC). The low-calcium diet group (LC) showed the lowest weight gain and had no differences among the groups with adequate calcium intake. Calcium retention was lowest in the LC group and higher in the CL, SC, AC groups than in SC groups. Serum alkaline phosphatase (ALP) level was highest in LC group, and significantly low in the CC and AC groups (p<0.05). Parathyroid hormone and osteocalcin levels showed no differences among experimental groups. The urine deoxypyridinoline (DPD) level was lower in AC and CC groups compared to the LC group (p<0.05). The dry weight of the femur showed no significant differences among normal calcium groups. The bone mineral density of the femur in AC and CC group were significantly higher than the LC group (p<0.05). From these results, calcium extracted from salted anchovy can be useful as a calcium supplement comparable with calcium carbonate.

멸치젓으로부터 추출한 칼슘이 체내의 칼슘대사에 미치는 영향을 알아보기 위하여 흰쥐를 대상으로 칼슘급원을 달리하여 섭취시킨 후 체내의 골격대사에 관련된 지표를 비교 평가하였다. 식이구성은 탄산칼슘군(calcium carbonate, CC), 젖산칼슘군(calcium lactate, CL), 해조칼슘군(seaweed calcium, SC), 멸치칼슘(anchovy calcium, AC), 저칼슘(low calcium, LC)군으로 총 5군으로 구성하였으며, 실험결과는 다음과 같다. 실험 종료까지의 체중은 탄산칼슘을 섭취한 CC군과 AC군에서 가장 많이 증가하였으며, LC군에서 체중 증가가 낮았고 식이섭취량도 LC군에서 가장 낮았으며, 정상칼슘군에서는 차이를 보이지 않았다. 칼슘 섭취량은 LC군에서 가장 낮은 수치를 나타내었고 정상칼슘군들 사이에는 유의적인 차이가 없었다. 칼슘 흡수율은 LC군에서 가장 높게 나타나 식이 칼슘수준이 적을수록 흡수율이 높아지는 것을 알 수 있었다. 칼슘 보유량은 저칼슘군에서 가장 낮았으며, CC군, CL군, AC군은 SC군에 비해 유의적으로 높은 수치를 나타내었다. 혈중 ALP 수준은 LC군에서 유의하게 높은 반면 정상칼슘군에서는 낮게 나타났으며, 특히 CC군과 AC군에서 현저히 낮게 나타났다. 칼시토닌 농도는 LC군에서 정상칼슘군보다 유의적으로 낮게 나타났고(p<0.05) 요중 DPD수치는 LC군에서 가장 높은 수치를 보였으며, CC군과 AC군에서 유의적으로 낮은 수치를 보였다. 대퇴골의 체중 100 g당 습윤무게는 실험군간에는 차이를 나타내지 않았으나, 건조무게는 정상칼슘군보다 LC군에서 유의적으로 낮은 결과를 보였다(p<0.05). 대퇴골의 골밀도는 LC군에서 정상칼슘군에 비해 유의하게 낮았고(p<0.05), 동일한 정상수준의 칼슘이 공급된 실험군 사이에서는 CL군에서 AC, CC, SC보다 유의적인 차이는 아니지만 감소경향을 보였다. 실험동물의 칼슘대사에 관련된 호르몬의 변화나 대퇴골의 골밀도 결과로 보아 멸치젓에서 추출한 칼슘은 기존에 사용해오던 탄산칼슘 수준으로 칼슘대사에 긍정적인 가치를 부여할 수 있을 것으로 사료되고, 멸치 부산물을 활용한 칼슘제 개발에 응용할 수 있을 것으로 본다.

Keywords

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