Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Total Phenolics of Dried Platycodon grandiflorum and Its Effect on Growth of Human Cancer Cell Lines

건조 도라지 추출물 및 분획물의 총 페놀계 화합물 함량 및 인체 암세포 증식 억제효과

  • Hwang, Seong Yeon (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Choi, Hyang Mi (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Lim, Sun-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 황성연 (한국해양대학교 해양환경생명과학부) ;
  • 최향미 (한국해양대학교 해양환경생명과학부) ;
  • 임선영 (한국해양대학교 해양환경생명과학부)
  • Received : 2012.07.31
  • Accepted : 2012.12.11
  • Published : 2013.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated the effects of extracts from dried Platycodon grandiflorum on total phenolic content and growth of cancer cell lines (HT-29 and AGS). Total phenolic contents of acetone/methylene chloride (A+M) extract and methanol (MeOH) fraction were 4.53 and 27.22 TAE mg/100 g, respectively. Among the fractions, n-butanol (n-BuOH) fraction contained the highest phenolic content. Treatments of crude extracts and fractions significantly inhibited the growth of HT-29 and AGS cancer cell lines (p<0.05). Among the fractions, n-BuOH fraction exhibited the highest inhibitory effect and was then sub-fractionated by reverse phase flash column chromatography (rfc). The rfc 1-3 exhibited a higher inhibitory effect on proliferation of both cancer cells. The rfc 1 contained the highest phenol content. Our results showed that n-BuOH fraction possessed a potent inhibitory effect on proliferation of human cancer cells. We suggested that this anticancer activity was partially related to the content of phenolic compounds.

본 연구에서는 도라지를 건조한 후 분말화하여 유기용매로 추출하여 도라지 추출물 및 분획물의 총 페놀계 화합물 함량 및 인체 암세포에 대한 증식 억제효과에 대해 검토하였다. 건조 도라지의 총 페놀계 화합물 함량 측정 실험에서 A+M 추출물과 MeOH분획물은 각각 4.53 및 27.22 TAE mg/100 g의 페놀계 화합물 함량을 나타내었으며, 건조 도라지 분획물들 중 n-BuOH 분획물은 14.82 TAE mg/100 g로 가장 높은 페놀계 화합물 함량을 나타내었다. n-BuOH 분획물을 다시 RP flash column chromatography로 분획하여 얻은 분획물들(rfc 1-5) 중 rfc 1은 다른 분획물들보다 유의적으로 높은 페놀계 화합물 함량을(1.27 TAE mg/100 g) 나타냈다. 건조 도라지 추출물의 인체 암세포들(HT-29 및 AGS)에 대한 증식 억제효과 실험에서 A+M 추출물 및 MeOH 분획물은 인체 암세포 증식을 유의적으로 억제하였으며(p<0.05) 두 암세포에 대한 증식 억제효과에는 차이가 없었다. 건조 도라지 분획물들을 농도별로 처리하였을 때에도 n-BuOH 분획물의 $IC_{50}$이 0.06 mg/mL로 가장 활성이 높았고 n-BuOH 분획물의 rfc 1-5를 암세포에 0.001-0.05 mg/mL의 농도로 처리했을 때 rfc 1-3의 경우, 0.005 mg/mL 이상의 농도에서 높은 암세포 증식 억제효과를 나타내었다. 이상의 연구결과로부터 건조 도라지의 n-BuOH 분획물에 의한 강력한 암세포 증식 억제효과는 높은 함량의 페놀계 화합물에 기인한 것으로 여겨지며 향후 분획물의 분리 정제를 통한 새로운 기능성 물질의 개발이 필요할 것으로 사료된다.

Keywords

References

  1. Doll R, Peto R. The cause of cancer; quantitative estimate of avoidable risks of cancer in the United States today. J. Natl. Cancer Inst. 66: 1191-1308 (1981)
  2. Sugimura T, Sato S. Mutagens-carcinogens in foods. Cancer Res. 43: 2415s-2421s (1983)
  3. Renner HW. In vivo effect of single or combined dietary antimutagens on mutagens-induced chromosomal aberrations. Mutat. Res. 244: 185-188 (1990) https://doi.org/10.1016/0165-7992(90)90070-Z
  4. Biesalski HK. Free radical theory of aging. Curr. Opin. Clin. Nutr. 5: 5-10 (2002) https://doi.org/10.1097/00075197-200201000-00002
  5. Brannen AL. Toxicology and biochemistry of butylated hydroxy toluene and butylated hydroxy anisole. J. Am. Oil Chem. Soc. 52: 59-63 (1975) https://doi.org/10.1007/BF02901825
  6. Ito N, Fukushima S, Hasebawa A. Carcinogenicity of BHA in F344 rats. J. Natl. Cancer Inst. 70: 343-352 (1983)
  7. Kromhout D. Essential micronutrients in relation to carcinogenesis. Am. J. Clin. Nutr. 45: 1361-1467 (1987)
  8. Park SJ, Park DH, Kim SS, Gon J, Lee HY. Chemical compositions of fermented Codonopsis lanceolata. J. Food Sci. Nutr. 38: 396-400 (2009)
  9. Kang MH, Choi CS, Kim ZS, Chung HK, Min KS, Park CG, Park HW. Antioxidantive activities of ethanol extract prepared from leaves, seed branch, and aerial part of Crotalaria sessiflora L. Korean J. Food Sci. Technol. 34: 1098-1102 (2002)
  10. Kim AJ, Han MR, Jeoung KH, Cho JC, Park WJ, Han CW, Chang KH. Physiological evaluation of Korea ginseng, deoduk and doragi pickles. Korean J. Food Nutr. 4: 443-447 (2008)
  11. Kim HD, Lee JW. Analysis of components of saponin from ginseng, platycodon, and Japanese touchwood. Korean Soc. Food Nutr. 21: 91-96 (1972)
  12. Tada T, Kaneiwa Y, Shoji J, Shibat S. Saponins of the root of P. grandiflorum. Isolation and the structure of platycodin D. Chem. Pharm. Bull. 23: 2965-2969 (1975) https://doi.org/10.1248/cpb.23.2965
  13. Kang BY, Kim M, Bae SJ. Enhancement of antioxidation effect of Platycodon grandiflorum with vitamin C on the DLPC liposomes. J. Korean Soc. Food Sci. Nutr. 31: 506-510 (2002) https://doi.org/10.3746/jkfn.2002.31.3.506
  14. Nagao T, Matsuda H, Namba K, Kubo M. Immune pharmacological studies on platycodi radix II : Antitumor activity of inulin from Platicodi radix. Shoyakugaku Zasshi. 40: 375-380 (1986)
  15. Kim KK, Sung BY, Jung HS, Choi SY, Moon SB. A study on the thermal characteristics of the large low temperature vacuum dryer for biological drying. J. Korean Soc. Marine Eng. 24: 427- 434 (2000)
  16. Folin O, Denis W. On phosphotungstic-phosphomolylodic compounds as color reagents. J. Biol. Chem. 12: 239-249 (1912)
  17. Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival: Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89: 271-277 (1986) https://doi.org/10.1016/0022-1759(86)90368-6
  18. Jeong CH, Choi GN, Kim JH, Kwak JH, Kim DO, Kim YJ, Heo HJ. Antioxidant activities from the aerial parts of Platycodon grandiflorum. Food Chem. 118: 278-282 (2010) https://doi.org/10.1016/j.foodchem.2009.04.134
  19. Jang JR, Hwang SY, Lim SY. Inhibitory effect of extracts of platycodon grandiflorum (the ballon flower) on oxidation and nitric oxide production. Korean J. Food Preserv. 18: 65-71 (2011) https://doi.org/10.11002/kjfp.2011.18.1.065
  20. Kim EJ, Choi JY, Yu M, Kim MY, Lee S, Lee BH. Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Korean J. Food Sci. Technol. 44: 337-342 (2012) https://doi.org/10.9721/KJFST.2012.44.3.337
  21. Lee JY, Hwang WI, Lim ST. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J. Ethnopharmacol. 93: 409-415 (2004) https://doi.org/10.1016/j.jep.2004.04.017
  22. Frei B, Higdon JV. Antioxidant activity of tea polyphenols in vitro: Evidence from animal studies. J. Nutr. 133: 3275-3284 (2003)
  23. Kim YS, Lee BE, Kim KJ, Lee YT, Cho KB, Chung YC. Antitumor and immunomodulatory activities of the P. grandiflorum cultivated for more than 20 years. Yakhak Hoeji 42: 382-387 (1998)
  24. Lee JY, Hwang WI, Lim ST. Effect of Platycodon grandiflorum DC extract on the growth of cancer cell lines. Korean J. Food Sci. Technol. 30: 13-21 (1998)
  25. Shon MY, Seo JK, Kim HJ, Sung NJ. Antimutagenic effect of extract of Platycodon grandiflorum. Korean J. Food Sci. Technol. 33: 651-655 (2001)

Cited by

  1. Effect of Saponin Content and Antioxidant Activities of Platycodon grandiflorum Radix by Cutting Length vol.23, pp.5, 2015, https://doi.org/10.7783/KJMCS.2015.23.5.363
  2. Effect of Drying Methods on the Saponin and Mineral Contents of Platycodon grandiflorum Radix vol.46, pp.5, 2014, https://doi.org/10.9721/KJFST.2014.46.5.636