Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Inhibitory Effect of Rumex Crispus L. Fraction on Adipocyte Differentiation in 3T3-L1 Cells

소리쟁이 분획물의 지방세포 분화 억제 효과

  • Park, Sung-Jin (Department of Food Science and Technology, Keimyung University) ;
  • Choi, Jun-Hyeok (Department of Food Science and Technology, Keimyung University) ;
  • Jung, Yeon-Seop (Department of Food Science and Technology, Keimyung University) ;
  • Yu, Mi Hee (Department of Food Science and Technology, Keimyung University)
  • 박성진 (계명대학교 식품가공학 전공) ;
  • 최준혁 (계명대학교 식품가공학 전공) ;
  • 정연섭 (계명대학교 식품가공학 전공) ;
  • 유미희 (계명대학교 식품가공학 전공)
  • Received : 2012.08.16
  • Accepted : 2012.11.07
  • Published : 2013.02.28
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Abstract

The anti-obesity effect of ethanol xtract and their fractions from Rumex Crispus L. on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by suppressing adipocyte differentiation and lipid accumulation with Oil red O assay, western blot and real-time PCR analysis. Ethyl acetate fraction of Rumex crispus L. significantly inhibited adipocyte differentiation when treated during the adipocyte differentiation process, as assessed by measuring fat accumulation using Oil red O staining. In inducing differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, isobutylmethylxanthine (IBMX), dexamethasone- and insulin-along with ethyl acetate fraction residue processing treatment significantly decreased protein expression of obesity-related proteins, such as peroxisome-proliferators-activated-receptor-${\gamma}$ ($PPAR{\gamma}$) and CCAAT enhancer-binding-proteins ${\alpha}$ ($C/EBP{\alpha}$). These results indicate that ethyl acetate fraction of Rumex crispus L. is the most effective candidate for preventing obesity. However further studies will be needed to identify the active compounds that confer the anti-obesity activity of ethyl acetate fraction from Rumex crispus L.

소리쟁이 추출물 및 분획물의 3T3-L1 전지방세포의 분화에 미치는 영향을 확인한 결과, 소리쟁이의 hexane, ethyl acetate 분획물에 의해 지방세포의 lipid droplet의 형성이 유의적으로 억제되었으며, 소리쟁이 에탄올 추출물과 모든 분획물 10 ${\mu}g/mL$의 농도에서 중성지질(triglyceride)의 함량이 유의적으로 감소되었다. 또한 지방세포의 분화에 관여하는 전사인자인 $PPAR{\gamma}$, $C/EBP{\alpha}$의 단백질 발현은 10 ${\mu}g/mL$의 ethyl acetate, butanol 분획물에 의해 현저하게 감소되었다. 따라서 소리쟁이 추출물과 분획물 중 가장 활성이 우수한 ethyl acetate 분획물을 이용하여 지방분화에 관여하는 전사 인자인 $PPAR{\gamma}$, $C/EBP{\alpha}$, 그리고 SREBP1c 및 지질의 합성, 수송, 저장에 관여하는 ACS, FAS, FATP1, FABP4, Perilipin의 발현에 미치는 영향을 관찰한 결과, ethyl acetate 분획물은 유의적으로 모든 유전자의 발현을 억제시켰다. 따라서 소리쟁이의 ethyl acetate 분획물은 지방세포의 분화에 관여하는 전사인자 및 유전자들의 발현을 감소시킴으로써 항비만 효과가 있는 천연물 소재로 이용 가능할 것으로 생각된다.

Keywords

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